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pDule- Mj Acd A9
Plasmid#197651PurposeE. coli machinery vector for the genetic encoding of acridone at TAG codons using the M. jannaschii Acd-A9 synthetase/tRNA pairDepositorInsertsMj Acd A9 synthetase
Methanocaldococcus jannaschii tRNA
UseTagsNoneExpressionBacterialMutationY32A, L65D, F108L, Q109E, D158S, L162TPromoterGlnS and lppAvailable sinceApril 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
NdeI – SpeI(NheI) – BamHI/BglII - ANXA2 (23-339)
Plasmid#136546PurposeExpresses ANXA2 chimeras in bacterial cellsDepositorTypeEmpty backboneUseTagsANXA2 (23-339) and His-tagExpressionBacterialMutationPromoterT7Available sinceMay 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET-30a(+)-Syk-tSH2
Plasmid#111269Purposeexpress murine Syk tandem SH2 domains (Ser 8 to Gln 264), the unphosphorylated form, in E.coli strain Rosetta 2 (DE3)DepositorInsertmurine Syk tandem SH2 domains (Ser 8 to Gln 264), the unphosphorylated form (Syk Mouse)
UseTagsExpressionBacterialMutationPromoterT7Available sinceJuly 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCph8
Plasmid#50552PurposeExpresses Cph8 under a modified PLtetO-1 promoterDepositorInsertCph8
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceApril 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
pTHSSd_38
Plasmid#59961PurposeHigh core fragment expression (high resource allocator)DepositorInsertcore fragment
UseTagsExpressionBacterialMutationN/APromoterPJ23105Available sinceDec. 19, 2014AvailabilityAcademic Institutions and Nonprofits only -
pBbE5c-RFP
Plasmid#35278DepositorInsertRFP
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterlacUV5Available sinceFeb. 16, 2012AvailabilityAcademic Institutions and Nonprofits only -
pJMP2367
Plasmid#160076PurposeZ. mobilis CRISPRi, promoter C, empty guide, sfGFPDepositorInsertempty sgRNA
UseTagsExpressionBacterialMutationPromoterAvailable sinceJune 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET21-pelB-LaG-24
Plasmid#172750PurposeBacterial expression of HIS-tagged anti-GFP nanobody LaG-24; HIS tag preceded by HRV 3C/PreScission cleavage site.DepositorInsertLaG-24
UseTags6xHIS and pelB leader for periplasmic secretionExpressionBacterialMutationPromoterT7Available sinceAug. 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET21-pelB-LaG-9
Plasmid#172742PurposeBacterial expression of HIS-tagged anti-GFP nanobody LaG-9; HIS tag preceded by HRV 3C/PreScission cleavage site.DepositorInsertLaG-9
UseTags6xHIS and pelB leader for periplasmic secretionExpressionBacterialMutationPromoterT7Available sinceAug. 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pFTK049
Plasmid#171321PurposePart Plasmid Entry Vector (LVL0) from the Fungal Modular Cloning ToolKit.DepositorInsertmCherry fluorescent reporter
UseSynthetic BiologyTagsExpressionBacterial and YeastMutationn/aPromoterAvailable sinceDec. 22, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCARSF85
Plasmid#64694PurposeH6-TF-Smt3-GB1-NpuDnaEN102-CBDDepositorTypeEmpty backboneUseTagsCBD, HisTag, NpuDnaE-N102, Smt3, and TFExpressionBacterialMutationPromoterT7Available sinceSept. 11, 2015AvailabilityAcademic Institutions and Nonprofits only -
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pET3a-SF-LMNC-TS
Plasmid#169374PurposeE. coli expression plasmid for human LMNA residues 31-542, corresponding to the shorter lamin C isoform lacking the unstructured head, with an N-terminal splitFlAsH tag and a C-terminal TwinStrep tag.DepositorInsertLMNA residues 31-542 (LMNA Human)
UseTagsSplit-FlAsH Tag and TwinStrep TagExpressionBacterialMutationdeleted amino acid 1-30 (unstructured head domain…PromoterNative pET3a promotorAvailable sinceMay 27, 2021AvailabilityAcademic Institutions and Nonprofits only -
pHH0103_DNM2_Dynamin-N
Plasmid#110008PurposeProtein expression and purification of DNM2_Dynamin-NDepositorInsertDNM2_Dynamin-N (DNM2 Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceOct. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAY02
Plasmid#13043DepositorInsertMutS
UseTagsHis6ExpressionBacterialMutationT193TT to G193CT Phe to Ala mutationPromoterAvailable sinceOct. 16, 2006AvailabilityAcademic Institutions and Nonprofits only -
pEDL3
Plasmid#25092DepositorInsertEdge detector
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceJune 29, 2010AvailabilityAcademic Institutions and Nonprofits only -
pET20b-GpNLuc-6xHis
Plasmid#135932PurposeExpresses the GpNLuc LumiFluor in bacterial cells.DepositorInsertGpNLuc LumiFluor
UseTags6xHisExpressionBacterialMutationPromoterT7Available sinceFeb. 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
pJMP1055
Plasmid#119242Purposeconstruction intermediateDepositorInsertsgRNA NT1/RR1 (rfp)
UseTagsExpressionBacterialMutationPromoterAvailable sinceDec. 27, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcNB3
Plasmid#89243PurposeResurfaced Cell-penetrating B-lac nanobodyDepositorInsertpcNB3 (B-Lac)
UseTagsExpressionBacterialMutationPromoterT7Available sinceOct. 30, 2017AvailabilityAcademic Institutions and Nonprofits only
