Addgene

Showing: 11181 - 11200 of 27133 results

Results per page:

pMAL c2x Tral FL-15A

Plasmid

#113011

Purpose

For bacterial expression of MBP fusion of full-length Drosophila Tral with phosphomutations (15A)

Depositor

Terry Orr-Weaver

Article

Hara et al Elife. 2018 Feb 26;7. pii: 33150. doi: 1

Insert

full length tral (tral Fly)

Use

Tags

Expression

Bacterial

Mutation

15 PNG phosphorylation sites mutated to A

Promoter

Availability

Academic Institutions and Nonprofits only

Enlarge
pMAL c2x Tral FL

Plasmid

#113012

Purpose

For bacterial expression of MBP fusion of full-length Drosophila Tral WT

Depositor

Terry Orr-Weaver

Article

Hara et al Elife. 2018 Feb 26;7. pii: 33150. doi: 1

Insert

full length tral (tral Fly)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

Enlarge
pET28 His10-FcRɣ

Plasmid

#113021

Purpose

for purification of His10-FcRɣ intracellular domain

Depositor

Ron Vale

Article

Morrissey et al Elife. 2018 Jun 4;7. pii: 36688. doi: 10

Insert

His10-FcRɣ intracellular domain

Use

Tags

Expression

Bacterial

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

Enlarge
pGEX6-2 SNAP-Syk tSH2

Plasmid

#113028

Purpose

for purification of Syk tSh2. Purified protein will have a snap tag that can be conjugated to fluorescent dyes.

Depositor

Ron Vale

Article

Morrissey et al Elife. 2018 Jun 4;7. pii: 36688. doi: 10

Insert

SNAP-Syk tSH2

Use

Tags

SNAP

Expression

Bacterial

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

Enlarge
pTL2-IncB-GFP11x7

Plasmid

#113031

Purpose

Expresses IncB tagged with 7 copies of GFP11 under a tetracycline inducible promoter

Depositor

Kevin Hybiske

Article

Wang et al Pathog Dis. 2018 Mar 1;76(2). pii: 48301

Insert

IncB (incB Chlamydia trachomatis)

Use

Tags

GFP11x7 and flag

Expression

Bacterial

Mutation

Promoter

tet inducible

Availability

Academic Institutions and Nonprofits only

Enlarge
His6-SpyCatcher-mi3

Plasmid

#113043

Purpose

Expresses His6-SpyCatcher-mi3 in bacterial cells as platform for plug-and-display vaccination

Depositor

Mark Howarth

Article

Bruun et al ACS Nano. 2018 Jul 20. doi: 10.1021/acsn

Insert

His6-SpyCatcher-mi3

Use

Tags

His-tag

Expression

Bacterial

Mutation

Promoter

T7

Availability

Academic Institutions and Nonprofits only

Enlarge
H6hSDHA

Plasmid

#113045

Purpose

Hetero expression of His-tagged human SDHA

Depositor

Edward A. Berry

Article

Zafreen et al Arch Biochem Biophys. 2016 Aug 15;604:47

Insert

H6hSDHA (SDHA Human)

Use

Tags

His6 (MGSSHHHHHHSQDP-)

Expression

Bacterial

Mutation

Insert begins at amino acid 43

Promoter

Availability

Academic Institutions and Nonprofits only

Enlarge
hSDH5

Plasmid

#113046

Purpose

Expression of WT hSDH5

Depositor

Edward A. Berry

Article

Zafreen et al Arch Biochem Biophys. 2016 Aug 15;604:47

Insert

hSDH5 (SDHAF2 Human)

Use

Tags

Expression

Bacterial

Mutation

Insert begins at amino acid 39

Promoter

Availability

Academic Institutions and Nonprofits only

Enlarge
hSDH5_G78R

Plasmid

#113047

Purpose

Expression of mutant hSDH5

Depositor

Edward A. Berry

Article

Zafreen et al Arch Biochem Biophys. 2016 Aug 15;604:47

Insert

hSDH5_G78R (SDHAF2 Human)

Use

Tags

Expression

Bacterial

Mutation

G78R, Insert begins at amino acid 39

Promoter

Availability

Academic Institutions and Nonprofits only

Enlarge
hSDH5_C83D

Plasmid

#113048

Purpose

Expression of mutant hSDH5

Depositor

Edward A. Berry

Article

Zafreen et al Arch Biochem Biophys. 2016 Aug 15;604:47

Insert

hSDH5_C83D (SDHAF2 Human)

Use

Tags

Expression

Bacterial

Mutation

C83D, Insert begins at amino acid 39

Promoter

Availability

Academic Institutions and Nonprofits only

Enlarge
pSumo24P7

Plasmid

#113072

Purpose

Plasmid for highly efficient expression of engineered IL24 with mutated binding sites

Depositor

Bohdan Schneider

Article

Zahradnik et al FEBS J. 2019 Oct;286(19):3858-3873. doi:

Insert

Human IL-24 engineered by computational design and fused with N-terminal SUMO tag (IL24 Human, SUMO part (Brachypodium distachyon))

Use

Tags

SUMO

Expression

Bacterial

Mutation

Q60R, K62E, K68E, K77R, M80L, S88D, A89V, Q93R, Q…

Promoter

T7

Availability

Academic Institutions and Nonprofits only

Enlarge
pSumo24P7vB4

Plasmid

#113073

Purpose

Plasmid for highly efficient expression of engineered IL24 with binding affinity to cognate receptors

Depositor

Bohdan Schneider

Article

Zahradnik et al FEBS J. 2019 Oct;286(19):3858-3873. doi:

Insert

Human IL-24 engineered by computational design and fused with N-terminal SUMO tag (IL24 Human, SUMO part (Brachypodium distachyon))

Use

Tags

SUMO

Expression

Bacterial

Mutation

Q60R, K62E, K68E, K77R, M80L, S88D, A89V, Q93R, Q…

Promoter

T7

Availability

Academic Institutions and Nonprofits only

Enlarge
pEvolvR-enCas9-PolI3M-TBD

Plasmid

#113077

Purpose

Expresses enCas9 fused to PolI3M-TBD in E. coli. contains a BsmbI-flanked GFP cassette for sgRNA cloning.

Depositor

David Schaffer, John Dueber

Article

Halperin et al Nature. 2018 Aug 1. pii: 10.1038/s41586-

Insert

enCas9-PolI3M-TBD

Use

Tags

Expression

Bacterial

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

Enlarge
pEvolvR-enCas9-PolI5M

Plasmid

#113078

Purpose

Expresses enCas9 fused to PolI5M in E. coli. contains a BsmbI-flanked GFP cassette for sgRNA cloning.

Depositor

David Schaffer, John Dueber

Article

Halperin et al Nature. 2018 Aug 1. pii: 10.1038/s41586-

Insert

enCas9-PolI5M

Use

CRISPR

Tags

Expression

Bacterial

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

Enlarge
pRS414-ATG41

Plasmid

#113080

Purpose

Contain 1,000 nucleotides of the ATG41 5'UTR, the ATG41 open reading frame and 500 nucleotides of the 3' UTR.

Depositor

Daniel Klionsky

Article

Yao et al Autophagy. 2015;11(12):2288-99. doi: 10.

Insert

ATG41

Use

Tags

Expression

Bacterial and Yeast

Mutation

Promoter

ATG41 endogenous promoter

Availability

Academic Institutions and Nonprofits only

Enlarge
T444T

Plasmid

#113081

Purpose

Enhanced RNAi vector for C. elegans. Contains two T7 polymerase termination sequences adjacent to the T7 promoter sites.

Depositor

Tibor Vellai

Article

Sturm et al Nucleic Acids Res. 2018 Jun 19. pii: 504

Type

Empty backbone

Use

RNAi

Tags

Expression

Bacterial

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

Enlarge
T777T

Plasmid

#113082

Purpose

Enhanced RNAi vektor for C. elegans. Contains two T7 polymerase termination sequences adjacent to the T7 promoter sites and a lacZ coding sequence.

Depositor

Tibor Vellai

Article

Sturm et al Nucleic Acids Res. 2018 Jun 19. pii: 504

Type

Empty backbone

Use

RNAi

Tags

Expression

Bacterial

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

Enlarge
pBLind

Plasmid

#113109

Purpose

Vector encoding the RFP gene under blue light-inducible promoter BindF1.

Depositor

Chueh Loo Poh

Article

Jayaraman et al ACS Synth Biol. 2018 Apr 20;7(4):986-994

Insert

RFP

Use

Cell-free

Tags

Expression

Bacterial

Mutation

Promoter

BindF1

Availability

Academic Institutions and Nonprofits only

Enlarge
pBAD-GINKO1

Plasmid

#113111

Purpose

Bacterial expression of genetically encoded green fluorescent potassium ion indicator GINKO1

Depositor

Robert Campbell

Article

Shen et al Commun Biol. 2019 Jan 14;2:18. doi: 10.1

Insert

GINKO1

Use

Tags

6-His Tag, T7 tag (gene 10 leader), and Xpress™ t…

Expression

Bacterial

Mutation

Promoter

araBAD

Availability

Academic Institutions and Nonprofits only

Enlarge
EcRho

Plasmid

#113121

Purpose

Expresses E. coli Rho

Depositor

James Berger

Article

Lawson et al Mol Cell. 2018 Sep 20;71(6):911-922.e4.

Insert

Rho

Use

Tags

MGH

Expression

Bacterial

Mutation

Promoter

T7

Availability

Academic Institutions and Nonprofits only

Enlarge