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Plasmid#170990PurposeFor bacterial expression of bdSUMO tagged muGFP with R9 peptide and NanoLuc complementation peptide variant #86DepositorInsert14x-His-bdSUMO-R9-muGFP-HiBiT
UseLuciferaseTagsExpressionBacterialMutationPromoterT7Available sinceSept. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
bdSUMO-pHD118-GFP-HiBit
Plasmid#171015PurposeFor bacterial expression of bdSUMO tagged muGFP with pHD118 peptide and NanoLuc complementation peptide variant #86DepositorInsert14x-His-bdSUMO-pHD118-muGFP-HiBiT
UseLuciferaseTagsExpressionBacterialMutationPromoterT7Available sinceSept. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
3BFN
Plasmid#60111PurposeBacterial expression for structure determination; may not be full ORFDepositorInsertKIF22:KIF22_dml-X01:C200692 (KIF22 Human)
UseTagsExpressionBacterialMutationSee commentsPromoterT7Available sinceOct. 22, 2014AvailabilityIndustry, Academic Institutions, and Nonprofits -
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pRSF-KpDcr1
Plasmid#32022DepositorInsertDCR1
UseTagsHis6-SUMOExpressionBacterialMutationPromoterT7Available sinceSept. 29, 2011AvailabilityAcademic Institutions and Nonprofits only -
pmcsg7-mCRT(WT)
Plasmid#83505PurposeBacterial expression of CalreticulinDepositorInsertCALR (Calr Mouse)
UseTagsHisExpressionBacterialMutationPromoterT7Available sinceOct. 12, 2016AvailabilityAcademic Institutions and Nonprofits only -
SNX11-PX (7-167)
Plasmid#119092PurposeBacterial expression of human phox homology (PX) domain, SNX11-PX (7-167)DepositorInsertSNX11-PX (7-167) (SNX11 Human)
UseTagsGSTExpressionBacterialMutationPromotertacAvailable sinceJuly 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pHAT5
Plasmid#112586PurposeUsed for expression of protein in E. coli as non-cleavable C-terminal 6His-tag fusionsDepositorTypeEmpty backboneUseTags6HisExpressionBacterialMutationPromoterAvailable sinceAug. 27, 2018AvailabilityAcademic Institutions and Nonprofits only -
Nanog-luciferase targeting construct
Plasmid#26591DepositorInsertNanog promoter-luciferase-lox-pgk-neomycin-loc
UseTagsExpressionBacterialMutationPromoterAvailable sinceOct. 22, 2010AvailabilityAcademic Institutions and Nonprofits only -
pQCascade-IS3
Plasmid#140626PurposeExpresses V. cholerae TniQ, Cas8, Cas7, and Cas6 and crRNA-IS3. The crRNA-IS3 targets IS3 loci in Escherchia coli.DepositorInsertVchTniQ, VchCas8, VchCas7, VchCas6, crRNA-IS3
UseCRISPR; TransposonTagsExpressionBacterialMutationPromoterAvailable sinceJuly 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET:GACG:GFP
Plasmid#60735PurposeA modified pET28 driving expression of GFP, biobrick part Bba_E0040. No LacO site downstream of PT7. T7 promoter has base pairs 'GACG' between -11 and -8.DepositorInsertGFP
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceNov. 5, 2014AvailabilityAcademic Institutions and Nonprofits only -
pTSlb-Q758C
Plasmid#60729PurposeExpresses both fragments of T7 RNAP split at position 179. The N-terminal fragment is driven by PLac while the C-terminal fragment is driven by PBAD. C-terminal fragment has the point mutations Q758CDepositorInsertsResidues 1-179 of split T7 RNAP
Residues 180-880 of split T7 RNAP
UseSynthetic BiologyTagsExpressionBacterialMutationResidues 1-180 of split T7 RNAP and Residues 180-…PromoterAvailable sinceNov. 4, 2014AvailabilityAcademic Institutions and Nonprofits only -
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pBabe-PI(WT)-Rac1(WT)-mCherry
Plasmid#87550PurposePI(WT): Photo-inhibitable/blue light sensitive Rac1DepositorInsertsUseTagsmCherryExpressionBacterial and MammalianMutationPromoterCMVAvailable sinceApril 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
PP-HSP90 (3O6O)
Plasmid#28153PurposeBacterial expression for structure determination; may not be full ORFDepositorInsertHSP90
UseTagsHis tag with TEV cleavageExpressionBacterialMutationN-terminal domainPromoterAvailable sinceFeb. 28, 2011AvailabilityIndustry, Academic Institutions, and Nonprofits -
p15aC-4D1D
Plasmid#107864PurposeBacterial expression of PIS and PI4KDepositorInsertsPIS
phosphatidylinositol 4-kinase β
UseTagsmycExpressionBacterialMutationPromoterproDAvailable sinceApril 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
L1-10XQUAS-R5
Plasmid#41435DepositorInsert10XQUAS
UseTagsExpressionBacterialMutationPromoterAvailable sinceNov. 30, 2012AvailabilityAcademic Institutions and Nonprofits only -
pQE60 Trx1 CCAAA
Plasmid#98402PurposeBacterial expression vector for Trx1 CCAAADepositorInsertTrx1 CCAAA (TXN Human)
UseTagsExpressionBacterialMutationC62A, C69A, C73APromoterAvailable sinceJuly 31, 2017AvailabilityAcademic Institutions and Nonprofits only -
pQE60 Trx1 SSAAA
Plasmid#98403PurposeBacterial expression vector for Trx1 SSAAADepositorInsertTrx1 SSAAA (TXN Human)
UseTagsExpressionBacterialMutationC32S, C35S, C62A, C69A, C73APromoterAvailable sinceJuly 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
CUL3A-c013
Plasmid#53672Purposebacterial expression plasmidDepositorInsertCUL3A
UseTagsHis6-TEVExpressionBacterialMutationsolubilizing mutations I342R & L346DPromoterAvailable sinceJune 13, 2014AvailabilityAcademic Institutions and Nonprofits only