Skip to main content
This website uses cookies to ensure you get the best experience. By continuing to use this site, you agree to the use of cookies.

Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more

Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at [email protected]. Learn more

Addgene
Showing: 10881 - 10900 of 27133 results

  2. pZJQIBEBT003

    Plasmid
    #140053
    Purpose
    The pZJQIBEBT003 plasmid contains the coding sequence of eMutS-L157C-G233C gene which has the ability of error removal in the gene synthesis process.
    Depositor
    Jia Zhang
    Article
    Zhang et al ACS Synth Biol. 2020 Apr 17;9(4):940-952
    Insert
    eMutS-L157C-G233C-CBM-EGFP
    Use
    Tags
    His-Tag
    Expression
    Bacterial
    Mutation
    L157C, G233C
    Promoter
    T7
    Availability
    Academic Institutions and Nonprofits only
    140053-plasmid-map-sequence-id-296195

    Enlarge

  3. pT7-[BsaI_cassette]-SpCas9_sgRNA_scaffold (MSP3485)

    Plasmid
    #140082
    Purpose
    Entry cloning vector for in vitro transcription or expression of SpCas9 sgRNAs from a T7 promoter
    Depositor
    Keith Joung
    Insert
    pT7-[BsaI_cassette]-SpCas9_sgRNA_scaffold (MSP3485)
    Use
    In vitro transcription (mrna synthesis)
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Availability
    Academic Institutions and Nonprofits only
    140082-plasmid-map-sequence-id-292704

    Enlarge

  16. pKM512

    Plasmid
    #140192
    Purpose
    For curing of Orbit-integrated plasmids and associated drug marker
    Depositor
    Kenan Murphy
    Article
    Murphy et al MBio. 2018 Dec 11;9(6). pii: mBio.01467-
    Inserts
    zeocin resistance gene
    Bxb1gp47 (directionality factor)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Cloned in gp47 directinality factor in place of R…
    Promoter
    EM7 and Ptet
    Availability
    Academic Institutions and Nonprofits only
    140192-plasmid-map-sequence-id-418051

    Enlarge

  17. LCB7_crtYB

    Plasmid
    #140232
    Purpose
    Expression of gene coding lycopene bifunctional β-cyclase for production beta-carotene from lycopene
    Depositor
    Kang Zhou
    Insert
    phytoene-beta carotene synthase
    Use
    Tags
    Expression
    Bacterial
    Mutation
    K307E
    Promoter
    pthrC3
    Availability
    Academic Institutions and Nonprofits only
    140232-plasmid-map-sequence-id-288146

    Enlarge

  18. TP2_crtYB

    Plasmid
    #140233
    Purpose
    Expression of gene coding lycopene bifunctional β-cyclase for production beta-carotene from lycopene
    Depositor
    Kang Zhou
    Insert
    phytoene-beta carotene synthase
    Use
    Tags
    Expression
    Bacterial
    Mutation
    na
    Promoter
    t7 promoter
    Availability
    Academic Institutions and Nonprofits only
    140233-plasmid-map-sequence-id-288147

    Enlarge

  19. F3 Neo* F3

    Plasmid
    #140235
    Purpose
    F3 (FRT variant)-flanked pPgk-pEM7-Neo*-bpA positive selection cassette
    Depositor
    Mario Capecchi
    Insert
    Neo*
    Use
    Mouse Targeting; Flp/ frt
    Tags
    Expression
    Bacterial and Mammalian
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    140235-plasmid-map-sequence-id-316186

    Enlarge

Showing: 10881 - 10900 of 27133 results