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EKAR2G_design1_mTFP_wt_Venus_wt

Plasmid

#39813

Depositor

Olivier Pertz

Article

Fritz et al Sci Signal. 2013 Jul 23;6(285):rs12. doi

Insert

Erk activity reporter

Use

Tags

His-Myc

Expression

Bacterial, Insect, and Mamm…

Mutation

Promoter

CMV

Available since

July 5, 2013

Availability

Academic Institutions and Nonprofits only

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pnYC-NpM-HsYTHDC2_36-1430_AD

Plasmid

#148487

Purpose

Bacterial Expression of HsYTHDC2_36-1430

Depositor

Elisa Izaurralde

Insert

HsYTHDC2_36-1430 (YTHDC2 Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pHH0103_TP53BP2-1/1

Plasmid

#91503

Purpose

Protein expression and purification of human SH3 domain construct TP53BP2-1/1

Depositor

Sachdev Sidhu

Article

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

TP53BP2-1/1 (TP53BP2 Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

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pHH0103_STAC3-2/2

Plasmid

#91466

Purpose

Protein expression and purification of human SH3 domain construct STAC3-2/2

Depositor

Sachdev Sidhu

Article

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

STAC3-2/2 (STAC3 Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

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pHH0103_SH3GL1-1/1

Plasmid

#91473

Purpose

Protein expression and purification of human SH3 domain construct SH3GL1-1/1

Depositor

Sachdev Sidhu

Article

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

SH3GL1-1/1 (SH3GL1 Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

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pHH0103_VAV2-1/2*

Plasmid

#91483

Purpose

Protein expression and purification of human SH3 domain construct VAV2-1/2*

Depositor

Sachdev Sidhu

Article

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

VAV2-1/2* (VAV2 Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

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pHH0103_SASH3-1/1

Plasmid

#91434

Purpose

Protein expression and purification of human SH3 domain construct SASH3-1/1

Depositor

Sachdev Sidhu

Article

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

SASH3-1/1 (SASH3 Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

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pHH0103_YES1-1/1

Plasmid

#91439

Purpose

Protein expression and purification of human SH3 domain construct YES1-1/1

Depositor

Sachdev Sidhu

Article

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

YES1-1/1 (YES1 Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

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pHH0103_STAC3-1/2

Plasmid

#91271

Purpose

Protein expression and purification of human SH3 domain construct STAC3-1/2

Depositor

Sachdev Sidhu

Article

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

STAC3-1/2 (STAC3 Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

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pelB-MBP-DipA-ss

Plasmid

#137068

Purpose

E. coli expression clone (T7lac promoter) for mature DipA (aa 21-342) from B. burgdorferi with N-terminal PelB signal peptide + His10 + mature maltose binding protein + TEV protease cleavage

Depositor

Debra Hansen

Article

Robertson et al Sci Rep. 2019 Nov 26;9(1):17606. doi: 10

Insert

AAC66790.1 (BB_0418 Borrelia burgdorferi B31)

Use

Tags

PelB signal peptide + His10 + maltose binding pro…

Expression

Bacterial

Mutation

mature DipA: lacks the DipA signal peptide

Promoter

T7lac

Available since

April 17, 2020

Availability

Academic Institutions and Nonprofits only

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pCAD54_CAD29_H104A_pvp008

Plasmid

#124876

Purpose

Expresses hCAD His103Ala mutant in E.coli

Depositor

Konrad Buessow

Article

Chen et al Proc Natl Acad Sci U S A. 2019 Sep 23. p

Insert

cis-aconitate decarboxylase (ACOD1 Human)

Use

Tags

StrepTag and TEV site

Expression

Bacterial

Mutation

mutated His103 to Ala, deleted amino acids 1-3 an…

Promoter

T7

Available since

Sept. 16, 2019

Availability

Academic Institutions and Nonprofits only

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pRSM17

Plasmid

#101641

Purpose

Encodes the fluorescent protein eqFP486 for constitutive expression in E. coli.

Depositor

Thomas Hughes

Article

Molina et al J Phys Chem Lett. 2017 Jun 15;8(12):2548

Insert

eqFP486

Use

Tags

6XHis

Expression

Bacterial

Mutation

Promoter

Available since

Feb. 28, 2018

Availability

Academic Institutions and Nonprofits only

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p2CT-Cdi

Plasmid

#83044

Purpose

Expresses Corynebacterium diphtheriae Cas9 (CdiCas9)

Depositor

Jennifer Doudna

Article

Ma et al Mol Cell. 2015 Nov 5;60(3):398-407. doi:

Insert

CdiCas9

Use

Tags

His-MBP

Expression

Bacterial

Mutation

Promoter

Available since

Nov. 1, 2016

Availability

Academic Institutions and Nonprofits only
pFA6a-PATagRFP-KanMX6

Plasmid

#87027

Purpose

Tag S. pombe genes in C terminal with PATagRFP

Depositor

Julien Berro

Insert

PATagRFP

Use

Tags

Expression

Bacterial and Yeast

Mutation

Promoter

Available since

Feb. 22, 2017

Availability

Academic Institutions and Nonprofits only

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HECTD1

Plasmid

#25183

Purpose

Bacterial expression for structure determination; may not be full ORF

Depositor

Cheryl Arrowsmith

Insert

hectd1.1273.1343 (HECTD1 Human)

Use

Tags

His

Expression

Bacterial

Mutation

Promoter

Available since

Aug. 9, 2010

Availability

Industry, Academic Institutions, and Nonprofits
GST-PS-Sos1 (PRM) - Cys (XSB364)

Plasmid

#78506

Purpose

Construct for expressing Sos1 proline-rich motif in E. Coli, with a C-terminal Cys for labeling

Depositor

Ron Vale

Article

Su et al Science. 2016 Apr 29;352(6285):595-9. do

Insert

Sos1 -Cys (PRM) (SOS1 Human)

Use

Tags

GST followed by a prescission protease claevage s…

Expression

Bacterial

Mutation

Promoter

Available since

June 7, 2016

Availability

Academic Institutions and Nonprofits only

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pGEX p85a(N)-SH2

Plasmid

#46464

Depositor

Bruce Mayer, Leszek Kotula

Article

Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

Insert

p85a(N) (PIK3R1 Human)

Use

Tags

GST and PreScission

Expression

Bacterial

Mutation

contains amino acids 321-434

Promoter

Tac

Available since

July 23, 2013

Availability

Academic Institutions and Nonprofits only

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pGEX Cis1-SH2

Plasmid

#46417

Depositor

Bruce Mayer

Article

Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

Insert

Cis1 (SOCS1 Human)

Use

Tags

GST and PreScission

Expression

Bacterial

Mutation

contains amino acids 90-214

Promoter

Tac

Available since

Sept. 17, 2013

Availability

Academic Institutions and Nonprofits only

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dishevelled

Plasmid

#38342

Depositor

Norbert Perrimon

Article

Klingensmith et al Genes Dev. 1994 Jan;8(1):118-30.

Insert

dishevelled (dsh Fly)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Aug. 29, 2012

Availability

Academic Institutions and Nonprofits only
pOPINGS

Plasmid

#41121

Depositor

Ray Owens

Type

Empty backbone

Use

Tags

Expression

Bacterial, Insect, and Mamm…

Mutation

Promoter

Available since

March 26, 2013

Availability

Academic Institutions and Nonprofits only

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