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pKP660

Plasmid

#160812

Purpose

Expression of ExbB, ExbD proteins

Depositor

Kathleen Postle

Article

Ollis et al Mol Microbiol. 2009 Aug;73(3):466-81. do

Insert

exbB, exbD

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Nov. 4, 2020

Availability

Academic Institutions and Nonprofits only

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pSA107

Plasmid

#59785

Purpose

drives ZIF-1 protein in C. elegans ubiquitously

Depositor

Jeremy Nance

Article

Armenti et al Development. 2014 Dec;141(23):4640-7. do

Inserts

cdc-42 promoter (NEWENTRY Nematode)
zif-1 coding sequence (zif-1 )
unc-54 3' UTR

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 29, 2014

Availability

Academic Institutions and Nonprofits only

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DJ-1 TEV site pET15b

Plasmid

#60687

Purpose

Recombinant protein expression of Park7

Depositor

Mark Wilson

Article

Milkovic et al Protein Sci. 2015 Oct;24(10):1671-85. do

Insert

DJ-1 (PARK7 Human)

Use

Tags

6xHis

Expression

Bacterial

Mutation

TEV site was inserted (see comments below).

Promoter

T7

Available since

Oct. 31, 2014

Availability

Academic Institutions and Nonprofits only
pBbE6k-RFP

Plasmid

#35288

Depositor

Jay Keasling

Article

Lee et al J Biol Eng. 2011 Sep 20;5:12.

Insert

RFP

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

LlacO-1

Available since

Feb. 16, 2012

Availability

Academic Institutions and Nonprofits only

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TOPOII Pdx1 (DM#260)

Plasmid

#15025

Depositor

Douglas Melton

Insert

Pdx1 (Pdx1 Mouse)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 18, 2007

Availability

Academic Institutions and Nonprofits only
pAW204

Plasmid

#113244

Purpose

E. coli expression vector for IHFa F13A + IHFb

Depositor

Jennifer Doudna

Article

Wright et al Science. 2017 Sep 15;357(6356):1113-1118

Inserts

IHFa F13A (ihfA E. coli)
IHFb

Use

Tags

Expression

Bacterial

Mutation

F13A

Promoter

Available since

Aug. 17, 2018

Availability

Academic Institutions and Nonprofits only

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pDIV494

Plasmid

#177701

Purpose

Plasmid expressing optimized Cas9 and NAT marker and sgRNA targeting ADE2 locus in D. hansenii

Depositor

Uffe Mortensen

Article

Strucko et al Synth Biol (Oxf). 2021 Oct 12;6(1):ysab0

Inserts

Cas9
Nat
sgRNA Targeting ADE2 locus in D.hansenii: AGCTAAGCAGATTAATGCAT

Use

CRISPR

Tags

SV40

Expression

Bacterial and Yeast

Mutation

Promoter

RNR2p (Debaryomyces hansenii ), SNR52p (Candida s…

Available since

March 11, 2022

Availability

Academic Institutions and Nonprofits only

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PAS528 (TJF059)

Plasmid

#65602

Purpose

pETDuet-1 Containing FadD-His

Depositor

Pamela Silver

Article

Ford et al PeerJ. 2015 Jun 30;3:e1040. doi: 10.7717

Insert

FadD

Use

Tags

6x His

Expression

Bacterial

Mutation

Promoter

T7 lacO

Available since

June 2, 2015

Availability

Academic Institutions and Nonprofits only
pTKA001

Plasmid

#164577

Purpose

Expresses SARS-CoV-2 nsp7 from a pET46 backbone for IPTG-inducible bacterial expression

Depositor

Robert Kirchdoerfer

Article

Altincekic et al Front Mol Biosci. 2021 May 10;8:653148.

Insert

nsp7 (ORF1ab )

Use

Tags

6xHis, Ek, TEV

Expression

Bacterial

Mutation

Promoter

T7 Promoter

Available since

Jan. 25, 2021

Availability

Academic Institutions and Nonprofits only

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C1-62 pComb3xss anti-cFos nanobody

Plasmid

#145835

Purpose

Bacterial expression of anti-cFos (Human) recombinant llama nanobody for use as an immunolabel

Depositor

James Trimmer

Article

Dong et al Elife. 2019 Sep 30;8. pii: 48750. doi: 1

Insert

anti-cFos (Human) recombinant llama nanobody C1-62 (FOS L. glama (llama))

Use

Tags

6xHis-HA

Expression

Bacterial

Mutation

Promoter

lac

Available since

June 2, 2020

Availability

Academic Institutions and Nonprofits only

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dps-RFP

Plasmid

#63843

Purpose

This plasmid is designed as a bacterial oxidative stress sensor

Depositor

Howard Salis

Insert

dps, mRFP1 and GFPmut3b

Use

Tags

mRFP1

Expression

Bacterial

Mutation

Gene is transcriptionally fused to RFP

Promoter

Available since

March 27, 2020

Availability

Academic Institutions and Nonprofits only

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katG-RFP

Plasmid

#63844

Purpose

This plasmid is designed as a bacterial oxidative stress sensor

Depositor

Howard Salis

Insert

katG, mRFP1 and GFPmut3b

Use

Tags

mRFP1

Expression

Bacterial

Mutation

Gene is transcriptionally fused to RFP

Promoter

Available since

March 27, 2020

Availability

Academic Institutions and Nonprofits only

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pET21a - Mtb-TF

Plasmid

#111513

Purpose

Expresses M. tuberculosis Trigger Factor with a C-terminal His tag

Depositor

Judy Lieberman

Article

Dotiwala et al Cell. 2017 Nov 16;171(5):1125-1137.e11.

Insert

Trigger Factor

Use

Tags

His tag

Expression

Bacterial

Mutation

Promoter

T7

Available since

June 25, 2018

Availability

Academic Institutions and Nonprofits only

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pHEN-CoV2-nanobody-Ty1

Plasmid

#194588

Purpose

Bacterial expression of Ty1 nanobody targeting the receptor binding domain (RBD) of SARS-CoV-2 spike

Depositor

Gerald McInerney

Article

Hanke et al Nat Commun. 2020 Sep 4;11(1):4420. doi:

Insert

Ty1 (S )

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Feb. 1, 2023

Availability

Academic Institutions and Nonprofits only

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pET_His6-MBP-TEV-Cas2

Plasmid

#186275

Purpose

Expresses Thiomicrospira Cas2 in E. coli

Depositor

Jennifer Doudna

Article

Wang et al Nat Commun. 2021 May 6;12(1):2571. doi:

Insert

Thiomicropira Cas2

Use

Tags

His6-MBP

Expression

Bacterial

Mutation

Promoter

Available since

July 13, 2022

Availability

Academic Institutions and Nonprofits only

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PARD6A_PDZ_1

Plasmid

#103946

Purpose

Protein expression and purification of TAX INTERACTION PROTEIN PDZ domain

Depositor

Sachdev Sidhu

Article

Tonikian et al PLoS Biol. 2008 Sep 30;6(9):e239. doi: 1

Insert

PARD6A (PARD6A Human)

Use

Tags

GST

Expression

Bacterial

Mutation

Promoter

tac

Available since

Aug. 29, 2018

Availability

Academic Institutions and Nonprofits only

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pBbB13k-GFP

Plasmid

#126122

Purpose

Constitutive expression vector for Escherichia coli. BglBrick compatible.

Depositor

Nigel Scrutton

Article

Jervis et al ACS Synth Biol. 2019 Mar 18. doi: 10.102

Insert

GFPuv

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

J23102

Available since

June 18, 2019

Availability

Academic Institutions and Nonprofits only

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pET28-SUMO-KaiA

Plasmid

#68049

Purpose

Strain: AM5115, Used for protein purification

Depositor

Susan Golden

Article

Kim et al Proc Natl Acad Sci U S A. 2012 Oct 30;10

Insert

KaiA

Use

Tags

6xHis and SUMO

Expression

Bacterial

Mutation

Promoter

T7

Available since

Dec. 6, 2016

Availability

Academic Institutions and Nonprofits only

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pBad-HaloGFP-Na0.5

Plasmid

#198634

Purpose

Green fluorescent Ca2+ ion indicator for bacterial expression.

Depositor

Robert Campbell

Article

Zhu et al Nat Chem Biol. 2023 Jan;19(1):38-44. doi

Insert

HaloGFP-Na0.5

Use

Tags

Expression

Bacterial

Mutation

Promoter

araBAD promoter

Available since

April 14, 2023

Availability

Academic Institutions and Nonprofits only

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pBAD_GFP_mCherry_++

Plasmid

#187391

Purpose

arabinose-inducible expression of GFP and mCherry

Depositor

Roi Avraham

Insert

GFP, mCherry

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 8, 2022

Availability

Academic Institutions and Nonprofits only

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