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Plasmid#126968PurposeLevel 1 vector. sfGFP reporter. Origin R6K (pir conditional).DepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionBacterialMutationNonePromoterAvailable sinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only
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pAL-rfp_T
Plasmid#74075PurposepAL374 plasmid carrying the rfp (T) sgRNA targeting the template strand of rfp, SpecRDepositorInsertsgRNA rfp (T)
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterptacAvailable sinceApril 12, 2016AvailabilityAcademic Institutions and Nonprofits only -
pET MBP-FlAsH LIC cloning vector (MBP-FlAsH)
Plasmid#30185DepositorTypeEmpty backboneUseTagsBiotin-H6-MBP-TEV recognition and FlAsHExpressionBacterialMutationPromoterAvailable sinceAug. 17, 2011AvailabilityAcademic Institutions and Nonprofits only -
pWT050a
Plasmid#107899PurposeW2.4-2 plasmid. Contains PLacO-sgRNA1, PRha-sgRNA4 and is part of CAMERA 2.4DepositorInsertPLacO-sgRNA1, PRha-sgRNA4
UseTagsExpressionBacterialMutationPromoterAvailable sinceApril 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET21-10XHis-GST-HRV-Her2-dL5-Her2
Plasmid#85624PurposeBacterial expression vector for Z342-dL5(E52D)-Z342 affibody fusion protein (AFA) with N-terminal His10, GST and HRV3C cleavage site (MBIC5, dL5**, FAP, AffiFAP)DepositorInsert10XHis-GST-HRV-Her2-dL5-Her2 (EGFR Human, Schistosoma japonicum, Synthetic)
UseTags10XHis-GST-HRV and The Her2 Affibody and dL5 FAP …ExpressionBacterialMutationThe dL5** FAP is E50D, L89S, also known as E52D/L…PromoterT7Available sinceJuly 24, 2018AvailabilityAcademic Institutions and Nonprofits only -
pSB_init-Sb66
Plasmid#159422PurposeExpression of the anti-TGP (thermostable GFP) synthetic nanobody Sb66DepositorInsertSb66
UseTagsc-Myc and poly-HisExpressionBacterialMutationPromoterAvailable sinceApril 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
pZA-mCherry-R5
Plasmid#106258PurposeExpresses mCherry fused with silaffin-R5 silica affinity peptide under the control of TetODepositorInsertmCherry
UseTagsAdded silaffin R5 peptide to C terminus of mCherr…ExpressionBacterialMutationPromoterAvailable sinceOct. 22, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCHERRY8
Plasmid#24663DepositorInsertmCherry
UseTagsExpressionBacterialMutationPromoterAvailable sinceJuly 7, 2010AvailabilityAcademic Institutions and Nonprofits only -
pBEST-p15A-PLlacO1-lacI-T500
Plasmid#45393DepositorInsertlac repressor
UseTagsExpressionBacterialMutationPromoterPL-lacO1Available sinceJune 13, 2013AvailabilityAcademic Institutions and Nonprofits only -
5407 pClpB
Plasmid#1235DepositorInsertClpB
UseTagsExpressionBacterialMutationnonePromoterAvailable sinceJan. 6, 2005AvailabilityAcademic Institutions and Nonprofits only -
pSpyTag-GlpG
Plasmid#79054PurposeExpresses SpyTagged GlpG proteinDepositorInsertSpyTag-GlpG (glpG )
UseTags6xHis and SpyTagExpressionBacterialMutationGlpG protein employed here corresponds to residue…PromoterTrcAvailable sinceJune 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
pGEM-T Dmel Or82a
Plasmid#59678PurposeProbe for Drosophila melanogaster Or82a expressionDepositorInsertOr82a (Or82a Fly)
UseTagsExpressionBacterialMutationPromoterAvailable sinceOct. 24, 2014AvailabilityAcademic Institutions and Nonprofits only -
pGEX6p-Meis1a
Plasmid#78144Purposebacterial expression of GST-Meis1aDepositorInsertMeis1a (Meis1 Mouse)
UseTagsGSTExpressionBacterialMutationPromoterAvailable sinceJune 10, 2016AvailabilityAcademic Institutions and Nonprofits only -
pGEX6P1-hPREP
Plasmid#179387PurposeExpress human prolyl oligopeptidase (tagged) in bacterial cellsDepositorInserthuman prolyl oligopeptidase (PREP Human)
UseTagsGSTExpressionBacterialMutationPromoterTACAvailable sinceApril 20, 2022AvailabilityAcademic Institutions and Nonprofits only -
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pCDFDuet-1 MKK4(EE)-MKK7a1(EE)
Plasmid#47580PurposeBacterial expression plasmid for co-expression of constitutively active MKK4 and MKK7a1DepositorUseTagsExpressionBacterialMutationLacks the first 36 residues of the RefSeq protein…PromoterAvailable sinceSept. 19, 2013AvailabilityAcademic Institutions and Nonprofits only -
p_SNIPR_target_GUmut33AtoG
Plasmid#139468PurposeT7 RNAP-driven expression of a non-cognate SNIPR trigger RNA with a mutation at position 33DepositorInsertTarget_mutGU33
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 19, 2020AvailabilityAcademic Institutions and Nonprofits only -
IBMc071
Plasmid#161943PurposeContains Type IIS restriction site free elements for expressing protein to be split in IBM. ECF20 expression plasmidDepositorInsertpSB3T5(BsaI-)-PBAD(SapI-)-B33-ECF20-T
UseTagsExpressionBacterialMutationPromoterPBADAvailable sinceMarch 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
NCOR2
Plasmid#40737PurposeBacterial expression for structure determination; may not be full ORFDepositorInsertNCOR2 (NCOR2 Human)
UseTags6xHis TEVExpressionBacterialMutationResidues 615 to 685 of the SANT2 domain of Nuclea…PromoterAvailable sinceOct. 18, 2012AvailabilityIndustry, Academic Institutions, and Nonprofits -
GST-Rab8a-QL
Plasmid#86079PurposeA bacterial expression plasmid encoding Rab8a GTPase-deficient mutant (Rab8a-Q67L) with N-terminus GST.DepositorInserthRab8A (RAB8A Human)
UseTagsGSTExpressionBacterialMutationChanged glutamine 67 to leucinePromotertacAvailable sinceMay 9, 2017AvailabilityAcademic Institutions and Nonprofits only