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Plasmid#120877PurposeExpresses concatenated noncoding sequences surrounding the CRISPR-Cas12c2 system in the native genomic loci; expresses the tracrRNA required for activity.DepositorInsertNoncoding sequences for CRISPR-Cas12c2
UseCRISPRTagsExpressionBacterialMutationPromoterlacAvailable sinceFeb. 21, 2019AvailabilityAcademic Institutions and Nonprofits only -
TLS 4: hTLS-CHOP.pBSKS(+)
Plasmid#21830DepositorInsertTLS-CHOP (FUS Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceSept. 10, 2009AvailabilityAcademic Institutions and Nonprofits only -
KLHL40 (KBTBD5A-c006)
Plasmid#98233PurposeBacterial expression for structure determination; may not be full ORFDepositorInsertKBTBD5A (KLHL40 Human)
UseTagsTEV-His6-FlagExpressionBacterialMutationPromoterT7Available sinceSept. 14, 2017AvailabilityAcademic Institutions and Nonprofits only -
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pelB-MBP-HtrA-ss
Plasmid#137066PurposeE. coli expression clone (T7lac promoter) for mature HtrA (aa 28-474) from B. burgdorferi with N-terminal PelB signal peptide + His10 + mature maltose binding protein + TEV protease cleavageDepositorInsertAAC66500.2 (BB_0104 Borrelia burgdorferi B31)
UseTagsPelB signal peptide + His10 + maltose binding pro…ExpressionBacterialMutationmature HtrA: lacks the HtrA signal peptidePromoterT7lacAvailable sinceApril 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pSR357.9
Plasmid#125105PurposeExpression of narL(1-129aa)-ydfI(124-213aa) chimera under PLtetO-1, output Promoter PydfJ115DepositorInsertsnarL(REC)-ydfI(DBD)129
sfgfp
tetR
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceMay 24, 2019AvailabilityAcademic Institutions and Nonprofits only -
pNBU2_erm_P1T_DP-B1
Plasmid#90319PurposeIntegration vector at an attB site in Bacteroides; contains the P1T_DP aTC-inducible promoter with the RBS B1DepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceMay 31, 2017AvailabilityAcademic Institutions and Nonprofits only -
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pST50Trc4-STRHISNDHFR
Plasmid#64000PurposePosition 4 transfer plasmid for pST44 polycistronic plasmid suite; N-term cleavable double tag; contains DHFR gene in BamHI-NgoMIV cassette as positive controlDepositorTypeEmpty backboneUseTags6xHis & Strep II (STR); TEV cleavableExpressionBacterialMutationPromoterT7Available sinceNov. 10, 2015AvailabilityAcademic Institutions and Nonprofits only -
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pETM41-EcPPXc
Plasmid#38329DepositorInsertE. coli polyphosphatase (ppx Escherichia coli)
UseTagsHis, MBP, and TEVExpressionBacterialMutationC-terminus of E. coli polyphosphatase (described …PromoterT7Available sinceAug. 6, 2012AvailabilityAcademic Institutions and Nonprofits only -
pHH0103_PPP1R13L-1/1
Plasmid#91509PurposeProtein expression and purification of human SH3 domain construct PPP1R13L-1/1DepositorInsertPPP1R13L-1/1 (PPP1R13L Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
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pMS6: pHelper(AvCAST)_entry_ΔTnsD
Plasmid#168139PurposeInducible expression of AvCAST proteins (except TnsD). Two BsaI sites for spacer cloning.DepositorInsertsAvCAST minimal CRISPR array
AvCAST Cascade proteins (Cas6, Cas8, Cas7 and Cas5)
AvCAST Tns proteins (TnsA, TnsB, TnsC and TniQ)
UseTagsExpressionBacterialMutationPromoterAvailable sinceJune 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET28a-DDX43-K292A
Plasmid#134575PurposeExpress human DDX43 mutant (K292A, ATPase dead) in E. coliDepositorInsertDDX43-K292A (DDX43 Human)
UseTagsHisExpressionBacterialMutationfull-length (K292A)PromoterT7Available sinceJan. 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMOLUC
Plasmid#12514DepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceNov. 13, 2006AvailabilityAcademic Institutions and Nonprofits only -
pMP89b HeEst
Plasmid#141206PurposeHalomonas elongata esterase with TEV cleaving site after the N-terminal Histag.DepositorInsertHeE
UseTagsExpressionBacterialMutationWTPromoterAvailable sinceJune 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBTBXh-4
Plasmid#26080DepositorTypeEmpty backboneUseTagsHisExpressionBacterialMutationPromoterAvailable sinceNov. 16, 2010AvailabilityAcademic Institutions and Nonprofits only -
pAtipiTrc
Plasmid#53275PurposeContains an Arabidopsis thaliana idi1 cDNA fused to a strong Trc promoter. Produces an active isopentenyl diphosphate isomerase (IDI) in E. coli.DepositorInsertipi1 (IPP2 Mustard Weed)
UseTagsExpressionBacterialMutationPromoterTrcAvailable sinceJan. 21, 2015AvailabilityAcademic Institutions and Nonprofits only -