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Plasmid#126603Purposemodular integrative vector for the assembly of DNA fragments and expression in Streptomyces speciesDepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceAug. 1, 2019AvailabilityAcademic Institutions and Nonprofits only
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pSL0373 (His-MBP-TniQ)
Plasmid#130638PurposeExpresses V. cholerae CAST TniQ from a T7 promoter, with N-terminal His10-MBP-TEVsite tag for purification.DepositorInsertVchTniQ
UseCRISPR; TransposonTagsHis10-MBP-TEVsite on TniQExpressionBacterialMutationPromoterT7Available sinceSept. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pJK408
Plasmid#72422PurposeProduces Acetobacter aceti 1023 Est10, C-terminal His5 tagDepositorInsertferuloyl esterase
UseTagsHis5ExpressionBacterialMutationPromoterT7Available sinceMarch 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
pET32a-Spike-403-505
Plasmid#165095Purposeprotein expression in E. coliDepositorInsertSevere acute respiratory syndrome coronavirus 2 spike protein site 403-505 (S SARS-CoV2)
UseTagsExpressionBacterialMutationPromoterAvailable sinceFeb. 10, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
pET His6 MBP TEV co-transformation cloning vector (13K-C)
Plasmid#48317DepositorTypeEmpty backboneUseTagsHis6 and MBPExpressionBacterialMutationPromoterAvailable sinceSept. 24, 2013AvailabilityAcademic Institutions and Nonprofits only -
pCAT221
Plasmid#134889PurposeBacterial expressionDepositorInserteGFP
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceJuly 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCB32
Plasmid#111911PurposeExpresses gRNA to target Nte1 locus and has HygR markerDepositorInsertgRNA cassette targeting Nte1 locus
UseCRISPRTagsExpressionBacterial and YeastMutationPromoterSNR52pAvailable sinceAug. 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
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pJMP1067
Plasmid#119243Purposerfp "test" strainDepositorInsertsgRNA NT1/RR1 (rfp)
UseTagsExpressionBacterialMutationPromoterAvailable sinceJan. 3, 2019AvailabilityAcademic Institutions and Nonprofits only -
21 pCOLI_A_N-GST_C_STREP
Plasmid#160258PurposeN-term GST 3C cleavable, C--term 2xStrepII Amp resistant bacterial expression vectorDepositorTypeEmpty backboneUseTags2xStrepII and GSTExpressionBacterialMutationPromoterT7Available sinceJan. 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
pPro18-cm
Plasmid#17807DepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceApril 28, 2008AvailabilityAcademic Institutions and Nonprofits only -
GST-PR-LBD
Plasmid#89117Purposebacterial expression of human progesterone receptor ligand binding domain amino acid residues 637-933 with GST tag in pGEX-3X-1DepositorInserthuman progesterone receptor ligand binding domain (PGR Human)
UseTagsGSTExpressionBacterialMutationcontains Ligand Binding Domain (637-933)PromotertacAvailable sinceApril 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
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pAP997-1
Plasmid#99500Purpose3XFlag::tagRFP::linker::TEVDepositorInsert3XFlag::tagRFP::linker::TEV
UseTagsExpressionBacterialMutationPromoterAvailable sinceSept. 8, 2017AvailabilityAcademic Institutions and Nonprofits only -
pMT-myl7-cytoBirA-2A-mCherry_Ras
Plasmid#80060PurposeMyl7 promoter driving HA-tagged biotin ligase (BirA), a Thosea asigna virus peptide (2A), and membrane-tethered mCherry protein (membCherry); flanked by Tol2 sequencesDepositorInsertBiotin ligase (BirA), a Thosea asigna virus peptide (2A), and mCherry protein
UseZebrafish biotaggingTags3x HAExpressionBacterialMutationPromoterMyl7 promoterAvailable sinceOct. 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
pMycVec1
Plasmid#32360DepositorInsertColE1 origin replication
UseTagsExpressionBacterialMutationPromoterAvailable sinceNov. 21, 2011AvailabilityAcademic Institutions and Nonprofits only -
pJ2048.2
Plasmid#140687Purpose3-color stripe (different sgRNAs)DepositorInsert3-color stripe (different sgRNAs)
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceJuly 13, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBAV-B
Plasmid#65928PurposeTheophylline Riboswitch B with lacZ geneDepositorInsertlacZ
UseTagsExpressionBacterialMutationPromoterAvailable sinceSept. 3, 2015AvailabilityAcademic Institutions and Nonprofits only -
pAC1748
Plasmid#127982PurposeAMA1 plasmid with Aspergillus optimized Cpf1, argB selection marker, and USER cassette (PacI/Nt.BbvCI)DepositorInsertCpf1
UseCRISPR; Fungal expressionTagsSV40 NLSExpressionBacterialMutationcodon optimized for Aspergillus nidulansPromoterAspergillus nidulans tef1 promoterAvailable sinceDec. 11, 2019AvailabilityAcademic Institutions and Nonprofits only