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Plasmid#73192PurposeTemplate plasmid which encodes kanamycin resistance gene for positive selection and toxin gene (chpB) under the control of rhamnose induceable promoter (PrhaB) for negative selection.DepositorInsertPrhaB-chpB (chpB )
UseTagsExpressionBacterialMutationPromoterPrhaBAvailable sinceJune 30, 2016AvailabilityAcademic Institutions and Nonprofits only -
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pKS913.CB18.31
Plasmid#69569PurposeTCR beta chain genomic transgenic construct for creating OT-1 miceDepositorInsertTCR beta (Tcrb Mouse)
UseMouse TargetingTagsExpressionBacterialMutationPromoterAvailable sinceOct. 21, 2015AvailabilityAcademic Institutions and Nonprofits only -
D. vulgaris sp2 CRISPR/pACYCDuet-1
Plasmid#81186PurposeExpresses CRISPR array containing 3 copies of Desulfovibrio vulgaris spacer 2 and flanking repeats in E. coliDepositorInsertSynthetic CRISPR array (3 copies of D. vulgaris spacer #2 flanked by repeats)
UseCRISPRTagsNoneExpressionBacterialMutationPromoterT7Available sinceSept. 15, 2016AvailabilityAcademic Institutions and Nonprofits only -
pET29b_Qcat18
Plasmid#45146DepositorInsertE-coli-pathways-QconCAT18
UseTags6XHisExpressionBacterialMutationPromoterT7Available sinceFeb. 5, 2014AvailabilityAcademic Institutions and Nonprofits only -
pET28-p53 (1-90) (72P)
Plasmid#62080PurposeExpression of human p53 (1-90) (72P) in e. coliDepositorInsertp53 (TP53 Human)
UseTagsHisExpressionBacterialMutationcontains TAD/PP residues 1-90; 72PPromoterT7Available sinceJan. 29, 2015AvailabilityAcademic Institutions and Nonprofits only -
APneg-VAMP1d
Plasmid#168010Purposenegative selection PACE Accessory PlasmidDepositorInsertVAMP1(T29-K89)-T7PAP
UseTagsExpressionBacterialMutationPromoterAvailable sinceMay 4, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGEX Emt-SH2
Plasmid#46424DepositorInsertEmt (ITK Human)
UseTagsGST and PreScissionExpressionBacterialMutationcontains amino acids 223-346PromoterTacAvailable sinceJuly 10, 2013AvailabilityAcademic Institutions and Nonprofits only -
pEA02
Plasmid#172193PurposeSuicide vector carrying the site attL of the SSR system of pSAM2. This plasmid is integrated into the genome of Actinobacteria by HR when carrying a DNA fragment identical to a region of the genome.DepositorInsertattL
UseTagsExpressionBacterialMutationPromoterAvailable sinceSept. 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
pEA03
Plasmid#172194PurposeSuicide vector carrying the site attR of the SSR system of pSAM2. This plasmid is integrated into the genome of Actinobacteria by HR when carrying a DNA fragment identical to a region of the genome.DepositorInsertattR
UseTagsExpressionBacterialMutationPromoterAvailable sinceSept. 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSC201
Plasmid#129387PurposeA variant of the pSCrhaB2 vector with the oriR6K and mob genes from pGPΩ-TP. Used for homologous recombination of the rhamnose-inducible system into the chromosome of Burkholderia cenocepacia K56-2.DepositorInsertoriR6K and oriT
UseTagsExpressionBacterialMutationN/APromoterN/AAvailable sinceSept. 30, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBAD/HisB (TIREVOL)-PAmCherry1
Plasmid#189718Purposetitratable expression of PAmCherry1 in bacteriaDepositorInsertPAmCherry1
UseTagsHis-T7-EKExpressionBacterialMutationPromoterpBADAvailable sinceNov. 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
pETM33_PTK2_Focal-AT
Plasmid#178485PurposeBacterial expression of human domain with His-tag and GST tagDepositorInsertPTK2_Focal-AT (PTK2 Synthetic, Human)
UseTagsHis-GSTExpressionBacterialMutationPromoterT7/LacOAvailable sinceMarch 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pALxR1
Plasmid#172564PurposeInducible gene expression, pACYC origin, LuxR activator, PLuxB promoter, mRFP, Chloramphenicol Resistance Marker.DepositorInsertLuxR-mRFP
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterPLuxBAvailable sinceNov. 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
pTHSSe_31
Plasmid#109235PurposePUPsp1 GFP reporterDepositorInsertsfGFP
UseTagsExpressionBacterialMutationPromoterPUPsp1Available sinceJuly 23, 2018AvailabilityAcademic Institutions and Nonprofits only -
pJRTCLCrVB1.3
Plasmid#22844DepositorInsertB DNA
UseCotton vigs vectorTagsExpressionBacterialMutation1.3 unit B componet length inserted into pBlueScr…PromoterAvailable sinceMarch 16, 2010AvailabilityAcademic Institutions and Nonprofits only -
pET12a-AL-103 FL
Plasmid#47090DepositorInsertAL-103 (IGKV3-20 Human)
UseTagsompTExpressionBacterialMutationPromoterT7Available sinceAug. 2, 2013AvailabilityAcademic Institutions and Nonprofits only -
His-PaIct
Plasmid#111293Purposebacterial expressionDepositorInsertsuccinyl-CoA:itaconate CoA transferase
UseTagsHis tagExpressionBacterialMutationPromoterAvailable sinceJan. 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCAH01SpR
Plasmid#128159PurposepCAH01 derivative (Ptet inducible promoter) with ahp (kanamycin resistance) CDS replaced with the aadA (streptomycin/spectinomycin resistance) CDSDepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterPtetAvailable sinceJuly 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
SLC9A3R1_PDZ_2
Plasmid#103933PurposeProtein expression and purification of SLC9A3R1 PDZ2 domainDepositorInsertSLC9A3R2 (NHERF2 Human)
UseTagsGSTExpressionBacterialMutationPromotertacAvailable sinceAug. 29, 2018AvailabilityAcademic Institutions and Nonprofits only