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p2CT-His-MBP-Lbu_C2c2_R472A_H477A

Plasmid

#83483

Purpose

Bacterial expression of L. buccalis C2c2 CRISPR effector (HEPN nuclease 1 inactive mutant).

Depositor

Jennifer Doudna

Article

East-Seletsky et al Nature. 2016 Sep 26. doi: 10.1038/nature

Insert

Lbu_C2c2_R472A_H477A

Use

CRISPR

Tags

His6-MBP

Expression

Bacterial

Mutation

R472A, H477A

Promoter

Available since

Oct. 31, 2016

Availability

Academic Institutions and Nonprofits only

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BB3aZ_14*

Plasmid

#98542

Purpose

empty BB3 for insertion and overexpression of 1 gene in P. pastoris

Depositor

Brigitte Gasser, Michael Sauer, Diethard Mattanovich

Article

Prielhofer et al BMC Syst Biol. 2017 Dec 8;11(1):123. doi

Type

Empty backbone

Use

Tags

Expression

Bacterial and Yeast

Mutation

Promoter

Available since

Feb. 20, 2018

Availability

Academic Institutions and Nonprofits only

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Terminase_PhiV1

Plasmid

#188836

Purpose

Large terminase from phage PhiV1

Depositor

Feng Zhang

Article

Gao et al Science. 2022 Aug 12;377(6607):eabm4096.

Insert

Terminase

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Aug. 17, 2022

Availability

Academic Institutions and Nonprofits only

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pHH0103_THRB_Hormone-recep

Plasmid

#109819

Purpose

Protein expression and purification of THRB_Hormone-recep

Depositor

Sachdev Sidhu

Article

Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

Insert

THRB_Hormone-recep (THRB Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 28, 2020

Availability

Academic Institutions and Nonprofits only

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pET30a-SARS-CoV2-ORF8

Plasmid

#165089

Purpose

protein expression in E. coli

Depositor

Rick Tarleton

Article

Shen et al medRxiv [Preprint]. 2021 Jan 27:2021.01.

Insert

Severe acute respiratory syndrome coronavirus 2 ORF8 (ORF8 SARS-CoV2)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Feb. 10, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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3535 pET15b Bid p22

Plasmid

#8784

Depositor

Stanley Korsmeyer

Article

Wei et al Genes Dev 2000 Aug 15;14(16):2060-71.

Insert

Bid p22 (Bid Mouse)

Use

Tags

His

Expression

Bacterial

Mutation

Promoter

Available since

July 6, 2005

Availability

Academic Institutions and Nonprofits only
pHM

Plasmid

#64173

Purpose

harbours marker genes HIS3 and MET17

Depositor

Markus Ralser

Article

Mulleder et al F1000Res. 2016 Sep 20;5:2351. eCollectio

Type

Empty backbone

Use

Tags

Expression

Bacterial and Yeast

Mutation

Promoter

Available since

Sept. 20, 2016

Availability

Academic Institutions and Nonprofits only

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pET42b-A3A-PBE-ΔUGI

Plasmid

#119771

Purpose

To express the recombinant APOBEC3A in vitro

Depositor

Caixia Gao

Article

Zong et al Nat Biotechnol. 2018 Oct 1. pii: nbt.426

Insert

APOBEC3A-nCas9-NLS

Use

Tags

6*HIS and NLS

Expression

Bacterial

Mutation

nCas9 has a mutation D10A in wild type SpCas9

Promoter

T7 promoter

Available since

June 28, 2019

Availability

Academic Institutions and Nonprofits only

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pET15b-12x

Plasmid

#61699

Purpose

Expresses PTDH mutant Opt12x

Depositor

Huimin Zhao

Article

Johannes et al Biotechnol Bioeng. 2007 Jan 1;96(1):18-2

Insert

Opt12x

Use

Tags

6xHis

Expression

Bacterial

Mutation

Promoter

T7

Available since

March 3, 2015

Availability

Academic Institutions and Nonprofits only
pET StrepII TEV cloning vector with BioBrick polycistronic restriction sites (9R)

Plasmid

#48290

Depositor

Scott Gradia

Type

Empty backbone

Use

Tags

StrepII

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 24, 2013

Availability

Academic Institutions and Nonprofits only

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B2-2

Plasmid

#111469

Purpose

Thr-tRNA synthetase

Depositor

Cheemeng Tan

Article

Villarreal et al Nat Chem Biol. 2018 Jan;14(1):29-35. doi

Insert

Thr-tRNA synthetase

Use

Tags

6xHis

Expression

Bacterial

Mutation

Promoter

Available since

June 19, 2018

Availability

Academic Institutions and Nonprofits only

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B4-2

Plasmid

#111476

Purpose

Asp-tRNA synthetase

Depositor

Cheemeng Tan

Article

Villarreal et al Nat Chem Biol. 2018 Jan;14(1):29-35. doi

Insert

Asp-tRNA synthetase

Use

Tags

6xHis

Expression

Bacterial

Mutation

Promoter

Available since

June 19, 2018

Availability

Academic Institutions and Nonprofits only

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B5-3

Plasmid

#111482

Purpose

Cys-tRNA synthetase

Depositor

Cheemeng Tan

Article

Villarreal et al Nat Chem Biol. 2018 Jan;14(1):29-35. doi

Insert

Cys-tRNA synthetase

Use

Tags

6xHis

Expression

Bacterial

Mutation

Promoter

Available since

June 19, 2018

Availability

Academic Institutions and Nonprofits only

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B8-3

Plasmid

#111495

Purpose

His-tRNA synthetase

Depositor

Cheemeng Tan

Article

Villarreal et al Nat Chem Biol. 2018 Jan;14(1):29-35. doi

Insert

His-tRNA synthetase

Use

Tags

6xHis

Expression

Bacterial

Mutation

Promoter

Available since

June 18, 2018

Availability

Academic Institutions and Nonprofits only

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B8-1

Plasmid

#111494

Purpose

Gly-tRNA synthetase B

Depositor

Cheemeng Tan

Article

Villarreal et al Nat Chem Biol. 2018 Jan;14(1):29-35. doi

Insert

Gly-tRNA synthetase B

Use

Tags

6xHis

Expression

Bacterial

Mutation

Promoter

Available since

June 18, 2018

Availability

Academic Institutions and Nonprofits only

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B8-4

Plasmid

#111496

Purpose

Gly-tRNA synthetase A

Depositor

Cheemeng Tan

Article

Villarreal et al Nat Chem Biol. 2018 Jan;14(1):29-35. doi

Insert

Gly-tRNA synthetase A

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

June 18, 2018

Availability

Academic Institutions and Nonprofits only

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B4-3

Plasmid

#111477

Purpose

Asn-tRNA synthetase

Depositor

Cheemeng Tan

Article

Villarreal et al Nat Chem Biol. 2018 Jan;14(1):29-35. doi

Insert

Asn-tRNA synthetase

Use

Tags

6xHis

Expression

Bacterial

Mutation

Promoter

Available since

June 18, 2018

Availability

Academic Institutions and Nonprofits only

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Phat2-dr-cPla2-C2-EGFP-6H

Plasmid

#187001

Purpose

Protein Expression

Depositor

Philipp Niethammer

Article

Shen et al Proc Natl Acad Sci U S A. 2022 Jan 4;119

Insert

ENSDARG00000024546 (pla2g4aa Zebrafish)

Use

Tags

EGFP

Expression

Bacterial

Mutation

Promoter

Available since

Aug. 3, 2022

Availability

Academic Institutions and Nonprofits only

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pUC19-P70a-Top-T~P70a-Bottom-T

Plasmid

#87314

Purpose

Expresses the modular Split-Broccoli system (Top + Bottom) under the control of E. coli sigma 70 promoters.

Depositor

Donald Burke

Article

Alam et al ACS Synth Biol. 2017 May 26. doi: 10.102

Insert

Split-Broccoli system

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

P70a

Available since

May 23, 2017

Availability

Academic Institutions and Nonprofits only

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pET21a - GR

Plasmid

#89614

Purpose

Expresses recombinant 6HIS tagged T. cruzi Glutathione reductase protein in E. coli upon IPTG induction

Depositor

Judy Lieberman

Article

Dotiwala et al Nat Med. 2016 Feb;22(2):210-6. doi: 10.1

Insert

Glutaredoxin

Use

Tags

6HIS

Expression

Bacterial

Mutation

Promoter

Available since

May 22, 2017

Availability

Academic Institutions and Nonprofits only

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