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BirA in pET28a (w400-2)

Plasmid

#26624

Depositor

Eric Campeau

Insert

BirA

Use

Tags

His

Expression

Bacterial

Mutation

Promoter

Available since

April 27, 2011

Availability

Academic Institutions and Nonprofits only

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pOlyA(E69A)

Plasmid

#132392

Purpose

Mutant version of OlyA that senses sphingomyelin without or with cholesterol

Depositor

Arun Radhakrishnan

Article

Endapally et al Cell. 2019 Feb 21;176(5):1040-1053.e17.

Insert

Ostreolysin A point mutant (E69A)

Use

Tags

His6 tag

Expression

Bacterial

Mutation

Mutations: C62S, E69A, C94S, S151C

Promoter

Available since

Sept. 16, 2020

Availability

Academic Institutions and Nonprofits only

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pNCS BFP

Plasmid

#91757

Purpose

Bacterial expression of a fluorescent protein, Blue Fluorescent Protein (BFP)

Depositor

Roger Tsien, Erik Rodriguez

Article

Cubitt et al Trends Biochem Sci. 1995 Nov;20(11):448-

Insert

Blue Fluorescent Protein

Use

Tags

Hexa-Histidine, Xpress epitope for detection with…

Expression

Bacterial

Mutation

GFP (Y66H)

Promoter

T7

Available since

June 8, 2017

Availability

Academic Institutions and Nonprofits only

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pPEP-TEV cNup98 (1-498)

Plasmid

#38037

Depositor

Roderick Lim

Article

Kowalczyk et al Nat Nanotechnol. 2011 Jun 19;6(7):433-8.

Insert

Nucleoporin 98 (NUP98 Human)

Use

Tags

His-tag, 3Cysteine

Expression

Bacterial

Mutation

amino acids 1-498

Promoter

T7

Available since

Sept. 14, 2012

Availability

Academic Institutions and Nonprofits only

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pUltra-chPheRS

Plasmid

#182257

Purpose

Genetic Incorporation of DOPA

Depositor

Han Xiao

Article

Chen et al J Mol Biol. 2021 Dec 20:167412. doi: 10.

Inserts

chPheRS2 for DOPA
chPheT for DOPA

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 3, 2022

Availability

Academic Institutions and Nonprofits only

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pQE-WT-RGVG-M6

Plasmid

#70172

Purpose

Expresses RGVG-M6 T7 RNA polymerase variant with enhanced transcription of 2' modified RNA

Depositor

Andrew Ellington

Article

Meyer et al Nucleic Acids Res. 2015 Sep 3;43(15):748

Insert

RGVG-M6 T7 RNA polymerase

Use

Tags

His

Expression

Bacterial

Mutation

Promoter

T5/Lac

Available since

Dec. 16, 2015

Availability

Industry, Academic Institutions, and Nonprofits

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pACYCDuet-1_Ec_rpoZ

Plasmid

#128837

Purpose

E. coli rpoz gene cloned between NcoI and HindIII sites of the first MCS of this Duet plasmid. Omega protein.

Depositor

Seth Darst

Article

Murakami et al J Biol Chem. 2013 Mar 29;288(13):9126-34

Insert

rpoZ (rpoZ E. coli)

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7

Available since

Aug. 20, 2019

Availability

Academic Institutions and Nonprofits only

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pDSG398

Plasmid

#115604

Purpose

surface-displayed Nb3; medium copy

Depositor

Ingmar Riedel-Kruse

Article

Glass et al Cell. 2018 Jul 26;174(3):649-658.e16. do

Insert

regulated adhesin construct

Use

Tags

Expression

Bacterial

Mutation

See depositor comments below

Promoter

Available since

Nov. 1, 2018

Availability

Academic Institutions and Nonprofits only

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human EB1 in PET28a for protein expression

Plasmid

#39297

Depositor

Jennifer Tirnauer, Tim Mitchison

Article

Tirnauer et al Mol Biol Cell. 2002 Oct . 13(10):3614-26

Insert

EB1 (MAPRE1 Human)

Use

Tags

6HIS

Expression

Bacterial

Mutation

Promoter

T7

Available since

Oct. 30, 2012

Availability

Academic Institutions and Nonprofits only
pET-PE2-His (IK1822)

Plasmid

#170103

Purpose

T7 promoter bacterial expression plasmid for PE2 (nSpCas9(H840A)-MMLV-RT) with C-terminal 6xHis-tag and bipartite NLS

Depositor

Keith Joung

Article

Petri et al Nat Biotechnol. 2021 Apr 29. pii: 10.103

Insert

bpNLS-nSpCas9(H840A)-MMLVRT-bpNLS-6xHis

Use

CRISPR

Tags

6xHis tag

Expression

Bacterial

Mutation

Bacteria codon-optimized nSpCas9 (H840A) & M-…

Promoter

T7

Available since

May 13, 2021

Availability

Academic Institutions and Nonprofits only

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pTXB1-pA-M.EcoGII

Plasmid

#192873

Purpose

pA-M.EcoGII expression plasmid, originally created for BIND&MODIFY method, a Long-range single-molecule mapping of chromatin modification in eukaryotes. doi: https://doi.org/10.1101/2021.07.08.451578

Depositor

Chong Tang

Article

Weng et al Genome Biol. 2023 Mar 29;24(1):61. doi:

Insert

pA-M.EcoGII

Use

Tags

3X Flag Tag

Expression

Bacterial

Mutation

Promoter

T7

Available since

Jan. 30, 2023

Availability

Academic Institutions and Nonprofits only

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pDSG360

Plasmid

#115601

Purpose

surface-displayed Ag3; medium copy

Depositor

Ingmar Riedel-Kruse

Article

Glass et al Cell. 2018 Jul 26;174(3):649-658.e16. do

Insert

regulated adhesin construct

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 5, 2018

Availability

Academic Institutions and Nonprofits only

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pCI-neo-cOVA

Plasmid

#25097

Depositor

Maria Castro

Article

Yang et al Proc Natl Acad Sci U S A. 2010 Mar 9. 10

Insert

cytoplasmic ovalbumin

Use

Tags

Expression

Bacterial and Mammalian

Mutation

Promoter

Available since

July 1, 2010

Availability

Academic Institutions and Nonprofits only

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pBA559

Plasmid

#186235

Purpose

pTet-LbuCas13a crRNA entry vector

Depositor

Jennifer Doudna

Article

Adler et al Nat Microbiol. 2022 Dec;7(12):1967-1979.

Type

Empty backbone

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 29, 2022

Availability

Academic Institutions and Nonprofits only

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pCS6

Plasmid

#55752

Purpose

Produces T7 RNA Polymerase.

Depositor

Matthew Bennett

Article

Schmidt et al ACS Synth Biol. 2012 Oct 19;1(10):445-50

Insert

T7 RNA Polymerase

Use

Tags

Expression

Bacterial

Mutation

Promoter

pBAD

Available since

July 15, 2014

Availability

Academic Institutions and Nonprofits only

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pMD19T-slr0168-Ptrc-GFP3mut-CmR

Plasmid

#73222

Purpose

Contains GFPmut3b under the Ptrc promoter. Suicide vector inserts into slr0168 site of Synechocystis. Carries chloramphenicol resistance. Propogates in E. coli

Depositor

Paul Hudson

Article

Yao et al ACS Synth Biol. 2015 Dec 28.

Insert

GFPmut3b

Use

Tags

Expression

Bacterial

Mutation

Promoter

Ptrc

Available since

April 26, 2016

Availability

Academic Institutions and Nonprofits only

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COVID-SARS2 NSP14/10

Plasmid

#159613

Purpose

Bacterial co-expression vector fo Covid-SARS2 NSP14 and NSP10

Depositor

Opher Gileadi

Article

Baddock et al Nucleic Acids Res. 2022 Jan 17. pii: 650

Inserts

Covid-SARS2 Nsp14 (ORF1ab Synthetic, COVID-SARS2)
Covid-SARS2 Nsp10

Use

Tags

His6, TEV cleavage site and none

Expression

Bacterial

Mutation

Codon-optimized for E. coli expression

Promoter

(Bicistronic) and T7 - LacO

Available since

Sept. 15, 2020

Availability

Academic Institutions and Nonprofits only

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pBAD-LSSmApple

Plasmid

#191259

Purpose

Bacterial expression plasmids for Long Stokes shift red fluorescent protein LSSmApple

Depositor

Robert Campbell

Article

Ejike et al Biochemistry. 2024 Jan 2;63(1):171-180.

Insert

LSSmApple

Use

Tags

His tag

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 19, 2022

Availability

Academic Institutions and Nonprofits only

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ER-GCaMP6f

Plasmid

#182548

Purpose

Expresses GCaMP6f on the cytosolic side of the ER (P-450site)

Depositor

Chris Richards

Article

Aryal et al Anal Chem. 2022 Jan 21. doi: 10.1021/acs

Insert

ER localized P450

Use

Tags

Expression

Bacterial and Mammalian

Mutation

Promoter

Available since

March 22, 2022

Availability

Academic Institutions and Nonprofits only

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pDD120

Plasmid

#119879

Purpose

Constitutive expression of Che9c60 and Che9c61 recombinases on pB264/pBR322 backbone with kanamycin resistance.

Depositor

Tae Seok Moon

Article

DeLorenzo et al ACS Synth Biol. 2018 Feb 16;7(2):727-738

Insert

pConstitutive_Che9c60 and Che9c61

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Feb. 22, 2019

Availability

Academic Institutions and Nonprofits only

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