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pCFPT7

Plasmid

#28012

Depositor

Lingchong You

Article

Tan et al Nat Chem Biol. 2009 Nov . 5(11):842-8.

Insert

T7 RNAP

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 23, 2011

Availability

Academic Institutions and Nonprofits only

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pET28a-MBP-CBX1-eReader

Plasmid

#135089

Purpose

Bacterial expression of engineered CBX1 with enhanced binding to H3K9me

Depositor

Marcey Waters

Article

Albanese et al ACS Chem Biol. 2020 Jan 17;15(1):103-111

Insert

CBX1 chromobox protein 1, engineered

Use

Tags

6xhis-tag and MBP

Expression

Bacterial

Mutation

lysine 43 mutated to alanine; aspartic acid 59 mu…

Promoter

T7

Available since

March 15, 2022

Availability

Academic Institutions and Nonprofits only

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6xHis-MBP-huPb2Cas12a

Plasmid

#174686

Purpose

Expresses 6xHis-MBP tagged Pb2Cas12a

Depositor

Piyush Jain

Article

Nguyen et al medRxiv 2021.07.21

Insert

huPb2Cas12a

Use

CRISPR and Synthetic Biology

Tags

6xHis, MBP, TEV and NLS, 6xHis, 3xHA

Expression

Bacterial

Mutation

Promoter

T7

Available since

Oct. 25, 2021

Availability

Academic Institutions and Nonprofits only

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6xHis-MBP-huLb5Cas12a

Plasmid

#174684

Purpose

Expresses 6xHis-MBP tagged Lb5Cas12a

Depositor

Piyush Jain

Article

Nguyen et al medRxiv 2021.07.21

Insert

huLb5Cas12a

Use

CRISPR and Synthetic Biology

Tags

6xHis, MBP, TEV and NLS, 6xHis, 3xHA

Expression

Bacterial

Mutation

Promoter

T7

Available since

Oct. 25, 2021

Availability

Academic Institutions and Nonprofits only

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pETM-60-NusA-DmBelle_B

Plasmid

#145963

Purpose

Bacterial Expression of DmBelle

Depositor

Elisa Izaurralde

Insert

DmBelle (bel Fly)

Use

Tags

Expression

Bacterial

Mutation

del 155-157, T530A and I685V

Promoter

Available since

July 6, 2021

Availability

Academic Institutions and Nonprofits only

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Orange Nano-lantern/pRSETB

Plasmid

#67659

Purpose

orange luminescent protein for high-speed single-cell and whole body imaging

Depositor

Takeharu Nagai

Article

Takai et al Proc Natl Acad Sci U S A. 2015 Apr 7;112

Insert

Orange Nano-lantern

Use

Tags

6xHis

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 10, 2015

Availability

Academic Institutions and Nonprofits only
pQZ083

Plasmid

#106417

Purpose

Express Complexin-1 fragment (1-83) in E. coli

Depositor

Axel Brunger

Article

Zhou et al Nature. 2017 Aug 24;548(7668):420-425. d

Insert

complexin-1 fragment 1-83 (Cplx1 Synthetic, Rat)

Use

Tags

GST tag

Expression

Bacterial

Mutation

Fragment 1-83, Codon optimization

Promoter

T7

Available since

Feb. 21, 2018

Availability

Academic Institutions and Nonprofits only

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pORTMAGE502B

Plasmid

#128971

Purpose

Broad-host-range GenR plasmid expressing PapRecT and EcMutL_E32K with Toluic acid induction

Depositor

George Church

Inserts

PapRecT
mutL E32K

Use

Tags

Expression

Bacterial

Mutation

E32K

Promoter

xyl-Pm

Available since

Aug. 7, 2019

Availability

Academic Institutions and Nonprofits only

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GST TopBP1 C (aa 978-1522)

Plasmid

#20373

Depositor

Aziz Sancar

Article

Choi et al Nucleic Acids Res. 2009 Jan 12. ():.

Insert

TopBP1 (978-1522) (TOPBP1 Human)

Use

Tags

Expression

Bacterial

Mutation

amino acids 978-1522

Promoter

Available since

March 5, 2009

Availability

Academic Institutions and Nonprofits only

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pWT037a

Plasmid

#107896

Purpose

W2.1 plasmid. Contains PTetO-SD8-BE2, PLacO-sgRNA1 and is part of CAMERA 2.1

Depositor

David Liu

Article

Tang et al Science. 2018 Feb 15. pii: science.aap89

Insert

PTetO-SD8-BE2, PLacO-sgRNA1

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

April 17, 2018

Availability

Academic Institutions and Nonprofits only

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pHH0103_GNAT3_G-alpha

Plasmid

#109948

Purpose

Protein expression and purification of GNAT3_G-alpha

Depositor

Sachdev Sidhu

Article

Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

Insert

GNAT3_G-alpha (GNAT3 Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 28, 2020

Availability

Academic Institutions and Nonprofits only

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pCMV-Sport6 Baf60b

Plasmid

#24723

Depositor

Benoit Bruneau

Article

Lickert et al Nature. 2004 Nov 4. 432(7013):107-12.

Insert

Baf60b (Smarcd2 Mouse)

Use

Tags

Expression

Bacterial and Mammalian

Mutation

Promoter

Available since

Aug. 2, 2010

Availability

Academic Institutions and Nonprofits only
pMRE150

Plasmid

#118500

Purpose

broad-host range plasmid to constitutively express fluorescent protein genes in bacteria

Depositor

Mitja Remus-Emsermann

Article

Schlechter et al Front Microbiol. 2018 Dec 12;9:3052. doi

Insert

TagBFP2

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 29, 2018

Availability

Academic Institutions and Nonprofits only

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pCoofy61

Plasmid

#122013

Purpose

E.coli vector for parallel SLIC cloning containing N-terminal His6-eGFP with PreScission Cleavage site

Depositor

Sabine Suppmann, Peggy Stolt-Bergner

Article

Scholz et al BMC Biotechnol. 2013 Feb 14;13(1):12.

Type

Empty backbone

Use

Tags

His6-eGFP

Expression

Bacterial

Mutation

Promoter

Available since

May 7, 2019

Availability

Academic Institutions and Nonprofits only

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pBAD-rxRFP1.2

Plasmid

#67839

Purpose

redox-sensitive red fluorescent protein rxRFP1.2

Depositor

Huiwang Ai

Article

Fan et al Anal Bioanal Chem. 2016 Jan 12.

Insert

rxRFP1.2

Use

Tags

His Tag

Expression

Bacterial

Mutation

Promoter

pBAD

Available since

Oct. 9, 2015

Availability

Academic Institutions and Nonprofits only
pT7-agrBD-I

Plasmid

#53439

Purpose

T7-dependent expression of the type-I agrB and agrD genes of S. aureus RN4220

Depositor

Cynthia Collins

Article

Marchand et al Biotechnol Bioeng. 2013 Nov;110(11):3003

Insert

agrBD locus

Use

Synthetic Biology

Tags

V5 epitope, 6x HIS (not expressed on agrB or agrD…

Expression

Bacterial

Mutation

none

Promoter

T7-lac

Available since

June 3, 2014

Availability

Academic Institutions and Nonprofits only

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pJMP1069

Plasmid

#119244

Purpose

rfp "test" strain

Depositor

Oren Rosenberg, Jason Peters, Carol Gross

Article

Peters et al Nat Microbiol. 2019 Jan 7. pii: 10.1038/

Insert

sgRNA NT1/RR1 (rfp)

Use

Tags

Expression

Bacterial

Mutation

D10A and H840A

Promoter

Available since

Feb. 4, 2019

Availability

Academic Institutions and Nonprofits only

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pGEX Crk-SH2

Plasmid

#46418

Depositor

Bruce Mayer

Article

Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

Insert

Crk (CRK Human)

Use

Tags

AviTag, GST, and PreScission

Expression

Bacterial

Mutation

contains amino acids 1-124

Promoter

Tac

Available since

July 12, 2013

Availability

Academic Institutions and Nonprofits only

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pDF0163 GwCas7x3 bacterial expression with Twinstrep-SUMO-Codon

Plasmid

#172504

Purpose

Encodes the GwCas7x3 protein only for bacterial recombinant protein expression for purification

Depositor

Jonathan Gootenberg, Omar Abudayyeh

Article

Ozcan et al Nature. 2021 Sep 6. pii: 10.1038/s41586-

Insert

GwCas7x3 bacterial expression with Twinstrep-SUMO-Codon

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 7, 2021

Availability

Academic Institutions and Nonprofits only

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NADK

Plasmid

#28268

Depositor

Cheryl Arrowsmith

Insert

NADK:HPC0AC-F12:C216828 (NADK Human)

Use

Tags

His

Expression

Bacterial

Mutation

contains residues 68-426.

Promoter

Available since

Feb. 10, 2012

Availability

Industry, Academic Institutions, and Nonprofits