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Plasmid#154959PurposeExpresses Nsp10 from SARS-CoV-2 with a N-terminal SUMO-protease cleavable 6His-SUMO tag in E. coliDepositorInsert6His-SUMO-Nsp10 (ORF1ab SARS-CoV-2)
UseTags6His-Thrombin-SUMOExpressionBacterialMutationPromoterT7 PromoterAvailable sinceAug. 5, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pCAG-PuroDTK.Neo
Plasmid#72307PurposePiggyBac Cassette contains puromycin and TK genesDepositorInsertPuromycin-DTK-neo
UseTagsExpressionBacterial and MammalianMutationPromoterCAG promoterAvailable sinceFeb. 15, 2017AvailabilityAcademic Institutions and Nonprofits only -
pBJ6
Plasmid#167135PurposeA gentamycin resistant plasmid for Tn7-mediated chromosomal integration of Photorhabdus luminescens luxCDABE operon with Pkan promoterDepositorInsertluxCDABE
UseLuciferaseTagsExpressionBacterialMutationPromoterKanamycin promoterAvailable sinceJune 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBJ3
Plasmid#167132PurposeA kanamycin resistant plasmid for Tn7-mediated chromosomal integration of Photorhabdus luminescens luxCDABE operon with Pfrr promoterDepositorInsertluxCDABE
UseLuciferaseTagsExpressionBacterialMutationPromoterFrr promoterAvailable sinceJune 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pON.mCherry
Plasmid#84821PurposeConstitutive Expression plasmid with mCherry fluorescent proteinDepositorInsertmCherry
UseTagsExpressionBacterialMutationPromoterTac PromoterAvailable sinceFeb. 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
pBJ5
Plasmid#167134PurposeA gentamycin resistant plasmid for Tn7-mediated chromosomal integration of Photorhabdus luminescens luxCDABE operon with Pfrr promoterDepositorInsertluxCDABE
UseLuciferaseTagsExpressionBacterialMutationPromoterFrr promoterAvailable sinceJune 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET21a.Tat(48-57)-I-SceI
Plasmid#165041PurposeExpression of Tat(48-57) conjugated I-SceI for protein purification (6XHis tag)DepositorInsertI-SceI
UseTags6XHis and Tat(48-57)ExpressionBacterialMutationPromoterAvailable sinceFeb. 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
p15A-OptoCre-knt-P*-R
Plasmid#189850PurposeCre-inducible activation of knt for kanamycin resistance.DepositorInsertP*-loxP-TT-loxP-R-knt
UseCre/Lox and Synthetic BiologyTagsExpressionBacterialMutationPromoterP* (modified T7 phage gene 10)Available sinceFeb. 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGST2-GST-TEV-OPTN
Plasmid#198428PurposeUsed for the expression and purification of OPTN (WT) (SMC Internal No. 1587)DepositorInsertoptineurin (OPTN Human)
UseTagsGST and TEVExpressionBacterialMutationPromoterAvailable sinceApril 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGLO-GFP-1UAG
Plasmid#82500PurposeExpresses GFP with 1 UAG codon at the amino acid position 3DepositorInsertGreen Fluorescent Protein with 1 UAG codon
UseTagsExpressionBacterialMutationAdded an UAG codon at the amino acid position 3PromoterAraBADAvailable sinceSept. 22, 2016AvailabilityAcademic Institutions and Nonprofits only -
pET-19b_MMLV-RT
Plasmid#153312PurposeBacterial expression of MMLV reverse transcriptaseDepositorInsertMMLV Reverse Transcriptase
UseTags10x HisExpressionBacterialMutationPromoterAvailable sinceJune 2, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pGEM4Z-mMcl-1
Plasmid#32984DepositorInsertMcl-1 (Mcl1 Mouse)
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 15, 2012AvailabilityAcademic Institutions and Nonprofits only -
Ube1/PET21d
Plasmid#34965DepositorInsertUbe1 (UBA1 Human)
UseTagsHisExpressionBacterialMutationPromoterT7Available sinceApril 19, 2012AvailabilityAcademic Institutions and Nonprofits only -
pT7-HISx6-SIMx6
Plasmid#116938PurposeBacterial expression vector for 6 simDepositorInsert6 sim
UseTagsExpressionBacterialMutationPromoterT7Available sinceDec. 21, 2018AvailabilityAcademic Institutions and Nonprofits only -
pT7-HISx6-SUMOx10
Plasmid#116937PurposeBacterial expression vector for 10 sumoDepositorInsert10 SUMO
UseTagsHISExpressionBacterialMutationPromoterT7Available sinceDec. 21, 2018AvailabilityAcademic Institutions and Nonprofits only -
pNBU2_repA1
Plasmid#211910PurposeReplicative vector for protein expression in Bacteroidales with an aTc inducible promoter, terminator, and a barcoding site.DepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceFeb. 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
RBM4_2xRRM-ZNF_pGEX
Plasmid#135154PurposeRecombinant expression of RBM4 with dual-affinity tagDepositorInsertRBM4 (RBM4 Human)
UseTagsGST-SBPExpressionBacterialMutationPromotertacAvailable sinceJune 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTriEx-mCherry-LOV2
Plasmid#81041PurposeMammalian or insect expression for wt LOV2DepositorInsertLOV2
UseTagsmCherryExpressionBacterial, Insect, and Mamm…MutationPromoterCMV, p10Available sinceAug. 17, 2016AvailabilityAcademic Institutions and Nonprofits only -
pET28a(ICOS)
Plasmid#177903PurposeBacterial expression of his10-tagged human ICOS Intracellular DomainDepositorInsertICOS (aa 162-199 only) (ICOS Human)
UseTags10xHis-Thrombin cut site-T7 tagExpressionBacterialMutationPromoterT7Available sinceDec. 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLIC-EcTOP
Plasmid#224463PurposeExpression of recombinant Escherichia coli topoisomerase IDepositorInsertEscherichia coli TopA
UseTags6xHis tagExpressionBacterialMutationPromoterT7Available sinceOct. 31, 2024AvailabilityAcademic Institutions and Nonprofits only