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Plasmid#12606DepositorInsertEscherichia coli surA (surA E. coli)
UseTagsinteinExpressionBacterialMutationwild typePromoterAvailable sinceOct. 16, 2006AvailabilityAcademic Institutions and Nonprofits only -
pBT3019
Plasmid#122541PurposeExpression of a C. elegan codon optimized florescent protein (CemOrange2) fused to a peroxisome target peptide (SKL) under the intestinal specific promoter nhx-2DepositorInsertPnhx-2_CemOrange2::SKL
UseTagsSKLExpressionBacterialMutationPromoterPnhx-2Available sinceMarch 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
KC1D_HUMAN_D0
Plasmid#79723PurposeThis plasmid encodes the kinase domain of KC1D. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.DepositorInsertKC1D (CSNK1D Human)
UseTagsHis10-TEVExpressionBacterialMutationPromoterT7Available sinceNov. 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
enz.105-MelA
Plasmid#154049PurposeExpresses MelA in E. coliDepositorInsertTyrosinase MelA
UseTagsHis, MBPExpressionBacterialMutationPromoterT7Available sinceMarch 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
hME-pOX
Plasmid#178181PurposeProduce cRNA that expresses a human MCU-EMRE fusion protein in Xenopus oocytesDepositorInserthuman MCU-EMRE fusion protein
UseTags1D4ExpressionBacterialMutationPromoterAvailable sinceOct. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
Spt2-GFP
Plasmid#115572PurposeExpresses yeast Spt2-GFP fusion proteinDepositorInsertSPT2
UseTagsGFPExpressionBacterial and YeastMutationPromoterAvailable sinceOct. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
4L59
Plasmid#60087PurposeBacterial expression for structure determination; may not be full ORFDepositorInsertL3MBTL3:JMC057-A08:C223215 (L3MBTL3 Human)
UseTags6xHisExpressionBacterialMutationSee commentsPromoterT7Available sinceOct. 22, 2014AvailabilityIndustry, Academic Institutions, and Nonprofits -
pECJ3B
Plasmid#75466PurposeToggle switch plasmid containing lacI-pdt3BDepositorInsertlacI-pdt3B
UseTagsExpressionBacterialMutationPromoterpLtetOAvailable sinceOct. 5, 2016AvailabilityAcademic Institutions and Nonprofits only -
bbCas9pluspAAA
Plasmid#82581PurposeThe plasmid encodes the T7 promoter, Cas9 (from pX330 #42230) and a stretch of poly-A. After linearization with restriction enzyme SapI It is used to produce in vitro transcribed mRNADepositorInsertCas9
UseTagsExpressionBacterialMutationPromoterT7Available sinceNov. 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET22-HT/prsA
Plasmid#73624PurposeProduces Escherichia coli phosphoribosylpyrophosphate synthetase (PrsA), N-terminal His6 tagDepositorInsertribose-phosphate diphosphokinase (prs Escherichia coli str. K-12 substr. MG1655)
UseTagsHis6ExpressionBacterialMutationPromoterT7Available sinceFeb. 22, 2016AvailabilityAcademic Institutions and Nonprofits only -
pET22b_PfSSB
Plasmid#103000PurposeThe expressed protein is a Plasmodium falciparum single-stranded DNA-binding protein, PfSSBDepositorInsertPfSSB
UseTagsExpressionBacterialMutationPromoterT7Available sinceMay 24, 2018AvailabilityAcademic Institutions and Nonprofits only -
17 pCOLI_A_SNAP
Plasmid#160254PurposeN-term His-SNAP 3C cleavable Amp resistant bacterial expression vectorDepositorTypeEmpty backboneUseTags6xHis and SNAPExpressionBacterialMutationPromoterT7Available sinceJan. 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
ERG_pet28a
Plasmid#131642PurposeBacterial expression of ETS gene ERGDepositorInsertERG (ERG Human)
UseTags6xHisExpressionBacterialMutationPromoterAvailable sinceOct. 3, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBR322 14-3-3 Promoter
Plasmid#16517DepositorInsert14-3-3 promoter (SFN Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceJuly 27, 2008AvailabilityAcademic Institutions and Nonprofits only -
pcDNA6.MCV.LT339.V5.4
Plasmid#28193DepositorInsertMCPyV Truncated Tumor Large T antigen (399 tumor strain)
UseTagsV5ExpressionBacterial and MammalianMutationPromoterAvailable sinceOct. 3, 2011AvailabilityAcademic Institutions and Nonprofits only -
pKM343
Plasmid#71487PurposeContains loxP-hyg-cat-loxP cassette for recombineering; CamR, HygR.DepositorInsertloxP-hyg-cat-loxP
UseSource of loxp-hyg-cat- loxp for pcr amplificatio…TagsExpressionBacterialMutationPromoterAvailable sinceJune 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET28b-GBP-MTn
Plasmid#154363PurposeBacterial expression of MTnDepositorInsertMTn
UseTagsHisExpressionBacterialMutationPromoterAvailable sinceAug. 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
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pRBBm69
Plasmid#48116PurposeShuttle vector E. coli/B.meg.DepositorInsertpromoter sacB - sacB
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceOct. 21, 2013AvailabilityAcademic Institutions and Nonprofits only -
pSL0747 (pDonor_L-MmeI)
Plasmid#130647PurposeEncodes a mini-transposon derived from V. cholerae Tn6677 CAST, with CmR cargo gene. The mutated left end introduces an MmeI recognition site (used for Tn-seq). Total transposon size = 977 bp.DepositorInsertVchCAST donor DNA (L*)
UseCRISPR; TransposonTagsExpressionBacterialMutationTGTTGATG --> TGTTGGAGPromoterAvailable sinceSept. 4, 2019AvailabilityAcademic Institutions and Nonprofits only