Showing: 9701 - 9720 of 27133 results
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Plasmid#58306PurposeGeneGuard genomic cassette: for constructing ΔdapA::RBS J pi, Kis, FRT-kanR-FRT (high-copy support for R6K COR). Will support pSEVAD1K.DepositorInsertGenomic cassette
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pSEVA12TEC-B
Plasmid#58307PurposeGeneGuard genomic cassette: for constructing ΔthyA::RBS B repA, Epsilon, FRT-kanR-FRT (low-copy support for ColE2 COR). Will support pSEVAT7Z.DepositorInsertGenomic cassette
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pSEVA12TEC-I
Plasmid#58308PurposeGeneGuard genomic cassette: for constructing ΔthyA::RBS I repA, Epsilon, FRT-kanR-FRT (medium-copy support for ColE2 COR). Will support pSEVAT7Z.DepositorInsertGenomic cassette
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pSEVA3b61
Plasmid#58310PurposeShuttle vector: pUC ori, pTHT15 ori for use in E. coli/B. subtilis. Cannot modify orientation of cat gene; promoter too weak in E. coli, probably transcribed via repB promoter in bicistronic manner.DepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pSEVA3b67Rb
Plasmid#58311PurposeShuttle vector: contains constitutive mRFP1, compatible as reporter for E. coli/B. subtilis. See pSEVA3b61 for more info.DepositorInsertmRFP1
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pWaldo-GFPd_PepTSt
Plasmid#58333PurposeExpresses the POT family transporter from Streptococcus thermophilus in E. coli as a C-terminally tagged GFP his tagged proteinDepositorInsertPepTSt
UseTagsGFP-HisExpressionBacterialMutationPromoterT7AvailabilityAcademic Institutions and Nonprofits only -
pWaldo-GFPe_PepTSo
Plasmid#58334PurposeExpresses the POT family transporter from Shewanella oniedensis in E. coli as a C-terminally tagged GFP his tagged proteinDepositorInsertPepTso
UseTagsGFP-HisExpressionBacterialMutationPromoterT7AvailabilityAcademic Institutions and Nonprofits only -
VV001: WT Arch-3 in pET28b
Plasmid#58487PurposeExpresses WT Archaerhodopsin-3 (with 6x C-terminal His tag) in E. coliDepositorInsertArchaerhodopsin-3 (with C-terminal His tag)
UseTagsExpressionBacterialMutationPromoterT7AvailabilityAcademic Institutions and Nonprofits only -
VV020: WT Arch-3 - eGFP in pET28b
Plasmid#58488PurposeExpresses WT Archaerhodopsin-3 fused to eGFP (with 6x C-terminal His tag) in E. coliDepositorInsertArchaerhodopsin-3
UseTagseGFPExpressionBacterialMutationPromoterT7AvailabilityAcademic Institutions and Nonprofits only -
pTRC-APX(W41F) dimeric
Plasmid#58499PurposeBacterial expression of APX W41F inducible by IPTGDepositorInsertpea APX W41F (dimeric)
UseTagsHis6ExpressionBacterialMutationW41FPromotertrcAvailabilityAcademic Institutions and Nonprofits only -
pLacO-ISce1
Plasmid#58505Purposeprovides 256 Lac operator repeats adjacent to the ISce1 restriction siteDepositorInsertLac repeats adjacent to 6 copies of the ISce1 restriction site
UseTagsExpressionBacterialMutationPromoterno promoterAvailabilityAcademic Institutions and Nonprofits only -
pTetO-ISce1-TetO
Plasmid#58506PurposeISce1 restriction site is flanked by Tet operator repeats, 96 on each sideDepositorInsertTet repeats flanking ISce1 restriction site
UseTagsExpressionBacterialMutationPromoterno promoterAvailabilityAcademic Institutions and Nonprofits only -
p005kanRFP
Plasmid#58533PurposeRFP from coral Corynactis sp. under T5 promoter with lac operators; kanamycin resistant.DepositorInsertRed fluorescent protein
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterT5 between two lac operatorsAvailabilityAcademic Institutions and Nonprofits only -
p006kanGFP
Plasmid#58534PurposeGFP from jellyfish Aquorea sp. under T5 promoter with lac operators; kanamycin resistant.DepositorInsertGreen fluorescent protein
UseTagsExpressionBacterialMutationPromoterT5 between two lac operatorsAvailabilityAcademic Institutions and Nonprofits only -
p007ampGFP
Plasmid#58535PurposeGFP from jellyfish Aquorea sp. under T5 promoter with lac operators; ampicillin resistant.DepositorInsertGreen fluorescent protein
UseTagsExpressionBacterialMutationPromoterT5 between two lac operatorsAvailabilityAcademic Institutions and Nonprofits only -
mouse ptc probe M2-3
Plasmid#58701Purposemakes mouse ptc probe for in situ hybridizationDepositorInsertptc (Ptch1 Mouse)
UseIn situ probeTagsExpressionBacterialMutationonly EcoRI fragment (5' end)PromoterT7AvailabilityAcademic Institutions and Nonprofits only -
pGEX mSmo-Cterm
Plasmid#58702Purposeused for anti-mouse Smoothened antibody production (for injection into animals)DepositorInsertSmo (Smo Mouse)
UseTagsGSTExpressionBacterialMutationcontains C terminus (aa 543-793)PromotertacAvailabilityAcademic Institutions and Nonprofits only -
pET28 mSmo-Cterm
Plasmid#58703Purposeused for anti-mouse Smoothened antibody production (for affinity purification)DepositorInsertSmo (Smo Mouse)
UseTags6X-HISExpressionBacterialMutationcontains C terminus (aa 543-793)PromoterAvailabilityAcademic Institutions and Nonprofits only -
pGEX mPtch-Cterm
Plasmid#58704Purposeused for anti-mouse Patched antibody production (for injection into animals)DepositorInsertPtch (Ptch1 Mouse)
UseTagsGSTExpressionBacterialMutationcontains only Cterminus (aa ~1238-1413)PromotertacAvailabilityAcademic Institutions and Nonprofits only -
pMal mPtch-Cterm
Plasmid#58705Purposeused for anti-mouse Patched antibody production (for affinity purification)DepositorInsertPtch (Ptch1 Mouse)
UseTagsMBPExpressionBacterialMutationcontains only Cterminus (aa ~1205-1434)PromoterAvailabilityAcademic Institutions and Nonprofits only
Showing: 9701 - 9720 of 27133 results