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Plasmid#182403PurposeBacterial expression of Vesicle Nucleating peptide-stefinA fusionDepositorInsertVNp-stefin (CSTA Human)
UseTagsVesicle Nucleating peptide (VNp)ExpressionBacterialMutationPromoterT7Available sinceFeb. 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pKS13
Plasmid#24627DepositorInsertpdc, adhB
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceJune 14, 2010AvailabilityAcademic Institutions and Nonprofits only -
CDK2_HUMAN_D0
Plasmid#79726PurposeThis plasmid encodes the kinase domain of CDK2. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.DepositorInsertCDK2 (CDK2 Human)
UseTagsHis10-TEVExpressionBacterialMutationPromoterT7Available sinceNov. 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
pQH
Plasmid#48096PurposeCumate-inducible expression of HA-tagged fusion proteinDepositorTypeEmpty backboneUseTags3xFLAG and HAExpressionBacterialMutationPromoterPQ5Available sinceDec. 6, 2013AvailabilityAcademic Institutions and Nonprofits only -
keratin 8 (krt8)-H2B-RFP
Plasmid#105955PurposetransgenesisDepositorInsertkrt8 (KRT8 Human)
UseTagsRFPExpressionBacterialMutationPromoterAvailable sinceFeb. 15, 2018AvailabilityAcademic Institutions and Nonprofits only -
NpT7-5-ERK1
Plasmid#39229DepositorInsertERK1 (MAPK3 Human)
UseTagsHisExpressionBacterialMutationPromoterT7Available sinceJuly 15, 2013AvailabilityAcademic Institutions and Nonprofits only -
pCRIS
Plasmid#120424PurposeThis plasmid encodes the complete CRISPR/dCas9 machinery for repressing transposition of the following bacterial Insertion Sequences: IS1, IS3, IS5, and IS150.DepositorInsertCRISPR spacers targeting IS1, IS5, IS3, IS150
UseCRISPR and Synthetic BiologyTagsExpressionBacterialMutationPromoterconstitutiveAvailable sinceJan. 17, 2019AvailabilityAcademic Institutions and Nonprofits only -
pETM33_AP2B1_adaptin
Plasmid#178467PurposeBacterial expression of human domain with His-tag and GST tagDepositorInsertAP2B1_adaptin (AP2B1 Human, Synthetic)
UseTagsHis-GSTExpressionBacterialMutationPromoterT7/LacOAvailable sinceMarch 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBtPIPLC His
Plasmid#173808PurposeExpression and purification of the mature form of Bacillus thuringiensis phosphaatidyl inositol specific phospholipase C (PI-PLC), UniProt ID P08954 from E. coli BL21 CodonPlus (DE3) RIL cellsDepositorInsertBacillus thuringiensis 1-phosphatidylinositol phosphodiesterase
UseTags6XHisExpressionBacterialMutationnonePromoterT7Available sinceOct. 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJ4M/TDP-43 S48E
Plasmid#104481Purposebacterial expression of TDP-43 S48EDepositorInsertTDP-43 (TARDBP Human)
UseTagsMBP-6xHisExpressionBacterialMutationS48EPromoterAvailable sinceMarch 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pJK100
Plasmid#73264PurposeProduces Bacillus subtilis phosphoribosylformylglycinamidine synthase small subunit (BsPurS)DepositorInsertphosphoribosylformylglycinamidine synthase subunit PurS
UseTagsExpressionBacterialMutationPromoterT7Available sinceFeb. 24, 2016AvailabilityAcademic Institutions and Nonprofits only -
Keasling-2187
Plasmid#100167PurposeUsed to improve production from engineered biosynthetic pathways include optimizing codon usage, enhancing production of rate-limiting enzymes, and eliminating the accumulation of toxic intermediates or byproducts to improve cell growthDepositorInsertMevT and MBIS operon
UseTagsExpressionBacterialMutationPromoterlacUV5Available sinceSept. 15, 2017AvailabilityAcademic Institutions and Nonprofits only -
pXRM15
Plasmid#63150PurposeMet15 marker pXR vector uses for both plasmid assembly in vivo, as well as targeted genomic integration.DepositorTypeEmpty backboneUseCre/LoxTagsExpressionBacterial and YeastMutationPromoterAvailable sinceApril 3, 2015AvailabilityAcademic Institutions and Nonprofits only -
pGEX-EGFAB(H306Y)
Plasmid#25771DepositorInsertLDL receptor EGFAB (LDLR Human)
UseTagsGSTExpressionBacterialMutationHis 306 to TyrPromoterAvailable sinceDec. 3, 2010AvailabilityAcademic Institutions and Nonprofits only -
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pGEX Stat1-SH2
Plasmid#46511DepositorInsertStat1 (STAT1 Human)
UseTagsGST and PreScissionExpressionBacterialMutationcontains amino acids 488-683PromoterTacAvailable sinceSept. 17, 2013AvailabilityAcademic Institutions and Nonprofits only -
Grp1-PH pET11a
Plasmid#71377PurposePhosphoinositide BindingDepositorInsertGrp1-PH (Cyth3 Mouse)
UseTagsExpressionBacterialMutationPleckstrin homology (PH) domain; aa 264-391PromoterT7Available sinceFeb. 26, 2016AvailabilityAcademic Institutions and Nonprofits only -
pBbB1k-GFP
Plasmid#35342DepositorInsertGFP
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterTrcAvailable sinceFeb. 20, 2012AvailabilityAcademic Institutions and Nonprofits only -
pET21b-LOV-ipaA
Plasmid#40236DepositorInsertLOV-ipaA
UseTags6xHisExpressionBacterialMutationJα helix region hybridized (AsLOV2 aa 537-546/ipa…PromoterT7Available sinceSept. 4, 2012AvailabilityAcademic Institutions and Nonprofits only -
pGMEH-P38-luxAB-ssrAec
Plasmid#27062DepositorInsertP#38-luxAB-ssrAec
UseTagsExpressionBacterialMutationPromoterAvailable sinceJuly 6, 2011AvailabilityAcademic Institutions and Nonprofits only