-
-
pET15b-hnRNP-F
Plasmid#23021DepositorInserthnRNP-F (HNRNPF Human)
UseTagsHisExpressionBacterialMutationPromoterAvailable sinceJune 4, 2010AvailabilityAcademic Institutions and Nonprofits only -
pJEC581
Plasmid#174369PurposeRFP reporter used to evaluate CRISPRa in bacteria, contains gRNA target sites every 10 bp upstream of the promoter.DepositorInsertRFP with PAM rich sequence upstream promoter
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterJ23117Available sinceOct. 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJEC625
Plasmid#174368PurposeGolden gate vector for gRNA cloning. Contains BbsI sites for insertion of the gRNA spacer as annealed oligos.DepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionBacterialMutationPromoterJ23119Available sinceOct. 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
pFCyR2
Plasmid#167513PurposeBroad-Host range expression vector, RSF1010 origin, CymRAM repressor, PCymRC promoter, mRFP, Kanamycin Resistance Marker.DepositorInsertCymRAM-mRFP
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterPCymRCAvailable sinceMay 28, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3/FLHIP1
Plasmid#29587DepositorInsertHuntingtin-interacting protein 1 (HIP1 Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceNov. 5, 2012AvailabilityAcademic Institutions and Nonprofits only -
pBR322-Pdp1_NTD-ZFN_L-HiBiT
Plasmid#198319PurposeLoads HiBiT-tagged L fragment of a zinc finger nuclease specific for human AAVS1 locus using Pdp1 packaging domainDepositorInsertPdp1_NTD-ZFN_L-HiBiT
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 29, 2023AvailabilityAcademic Institutions and Nonprofits only -
mCherryS131C
Plasmid#48732PurposeExpresses mCherryS131C in E. coli cellsDepositorInsertmCherryS131C
UseTags6xHisExpressionBacterialMutationchanged serine 131 to cysteinePromoterT5Available sinceOct. 9, 2013AvailabilityAcademic Institutions and Nonprofits only -
pDB.His.MBP.3C NLRP1 CARD WT
Plasmid#164008PurposeBacterial expression vector encoding a HRV 3C-cleavable His-MBP-tagged NLRP1 CARDDepositorInsertNLRP1 (NLRP1 Human)
UseTagsHis6-MBP-3CExpressionBacterialMutationPromoterAvailable sinceFeb. 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGSTag Flag Jnk3a2
Plasmid#15748DepositorInsertJnk3a2 (MAPK10 Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 6, 2008AvailabilityAcademic Institutions and Nonprofits only -
883 pET15b PTEN
Plasmid#10936DepositorInsertPTEN (PTEN Human)
UseTagsHisExpressionBacterialMutationPromoterAvailable sinceJan. 3, 2006AvailabilityAcademic Institutions and Nonprofits only -
FRB-polySH33
Plasmid#175233PurposeBacterial expression and purification, mixed with polyPRM to form condensates, can bind FKBP in the presence of rapamycinDepositorInsertNCK1 (NCK1 Human)
UseTagsExpressionBacterialMutationPromotertacAvailable sinceOct. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pKEW215_AcrIIA13
Plasmid#138281PurposeExpression of AcrIIA13 proteinDepositorInsertAcrIIA13
UseCRISPRTagsMBPExpressionBacterialMutationPromoterAvailable sinceMarch 13, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET His6 Sumo TEV cloning vector with BioBrick polycistronic restriction sites (9S)
Plasmid#48291DepositorTypeEmpty backboneUseTagsHis6 and SumoExpressionBacterialMutationPromoterAvailable sinceSept. 24, 2013AvailabilityAcademic Institutions and Nonprofits only -
pET-32a(+)-TGFB1m
Plasmid#120286PurposeProduce human recombinant mTGFB1 in DE3 cellsDepositorInsertmonomeric Transforming Growth Factor-β1
UseTags6x His and S-TagExpressionBacterialMutationCodon optimized for E. Coli productionPromoterT7 PromotorAvailable sinceJune 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBbB8c-GFP
Plasmid#35362DepositorInsertGFP
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterBADAvailable sinceMarch 2, 2012AvailabilityAcademic Institutions and Nonprofits only -
6xHis-TEV-UBC-9
Plasmid#87055PurposeExpresses 6xHis-tagged ubc-9, with a TEV protease cleavage site between the tag and the ORFDepositorInsertUBC-9 (ubc-9 Nematode)
UseTags6xHis-TEVExpressionBacterialMutationPromoterT7Available sinceApril 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCB410 (His-Ub-Ate1-4 1A7B)
Plasmid#104339PurposeExpress His Tag Ate1-4 1A7BDepositorInsertATE1 (Ate1 Mouse)
UseTags6xHis-UbExpressionBacterialMutationPromoterAvailable sinceJuly 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
-
eNS2B47NS3 L30SF31S
Plasmid#155319PurposeDENV4 47 peptides from NS2B cofactor linked to NS3 full length by VKTQR linker L30SF31S mutantDepositorInsertDENV4 NS3 full length protein linked with 47 peptides from NS2B cofactor
UseTagsHis tagExpressionBacterialMutationL30 to S, F31 to SPromoterM13Available sinceSept. 17, 2020AvailabilityAcademic Institutions and Nonprofits only
