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pHH0103_PCMT1_PCMT
Plasmid#109932PurposeProtein expression and purification of PCMT1_PCMTDepositorInsertPCMT1_PCMT
UseTagsExpressionBacterialMutationPromoterAvailable sinceOct. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGEM-hu FceRI beta
Plasmid#8366DepositorInserthuman Fc epsilon RI beta cDNA (MS4A2 Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceJune 7, 2005AvailabilityAcademic Institutions and Nonprofits only -
pMINTC3GH (Kan)
Plasmid#110076PurposeAnalogous to pMINTNH3 but with a C-terminal 3C cleavage site and a GFP-His10 tag fusion.DepositorTypeEmpty backboneUseTags3C cleavage site - GFP - His10 TagExpressionBacterialMutationPromoterAvailable sinceJune 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
Bluescript-OPNb
Plasmid#107979Purposeexpression of OPNbDepositorInsertosteopontin (OPNb)
UseTagsExpressionBacterialMutationPromoterAvailable sinceApril 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET15b+PCNA1 P186C
Plasmid#66173PurposeExpresses the P186C mutant of Sulfolobus solfataricus PCNA1DepositorInsertDNA polymerase sliding clamp 1
UseTagsHis6 tagExpressionBacterialMutationP186CPromoterT7 promoterAvailable sinceJune 29, 2015AvailabilityAcademic Institutions and Nonprofits only -
pReporter_8
Plasmid#60568PurposeA reporter plasmid containing the attB and attP sites flanking a green fluorescent protein reporter gene (gfp).DepositorInsertReporter_8
UseTagsExpressionBacterialMutationPromoterBBa_J23119Available sinceMarch 16, 2015AvailabilityAcademic Institutions and Nonprofits only -
pMAL-c5X-SELP0K
Plasmid#67007PurposeExpresses malE-SELP0K in E. coliDepositorInsertmalE-SELP0K
UseTagsHis6 and malE-Factor XaExpressionBacterialMutationPromotertacAvailable sinceJan. 5, 2016AvailabilityAcademic Institutions and Nonprofits only -
pEVOL-MmAcKRS1-PylTUUA
Plasmid#182537PurposeN‐acetyl lysyl‐tRNA synthetase with cognate tRNA for genetic code expansion with ochre suppressionDepositorInsertPylTUUA
UseTagsExpressionBacterialMutationPromoterProKAvailable sinceApril 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAC572
Plasmid#127973PurposeAMA1 plasmid with pyrG selection marker, and USER cassette (PacI/Nt.BbvCI)DepositorInsertpyrG
UseFungal expressionTagsExpressionBacterialMutationPromoternative promoterAvailable sinceAug. 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
pETM33_Nsp7
Plasmid#156476PurposeBacterial expression of Sars-CoV2 Nsp7 protein with His-tag and GST-tagDepositorInsertNsp7 (ORF1ab Synthetic, Severe acute respiratory syndrome coronavirus 2)
UseTagsHis-GSTExpressionBacterialMutationGene insert is codon optimized for Ecoli.PromoterT7/LacOAvailable sinceAug. 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
pKS1
Plasmid#24636DepositorInsert'tesA
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceJune 14, 2010AvailabilityAcademic Institutions and Nonprofits only -
pG1AK-PheB
Plasmid#71738PurposeModular shuttle vector for Geobacillus and E. coliDepositorInsertPheB
UseSynthetic Biology; Shuttle vectorTagsExpressionBacterialMutationPromoterpRplsWTAvailable sinceJuly 5, 2016AvailabilityAcademic Institutions and Nonprofits only -
pET28CLY6
Plasmid#21765DepositorInsertECFP -EYFP linked by flexible linker containing 6 SGGSGG repeats
UseTagsHisExpressionBacterialMutationPromoterAvailable sinceDec. 1, 2009AvailabilityAcademic Institutions and Nonprofits only -
pmCellFree_KA2_ZIKV NS4B
Plasmid#145367PurposeCell-free/mammalian expression vector for Zika virus NS4Bprotein with N-term mCherryDepositorInsertZIKV NS4B
UseTags8xHis and mCherryExpressionBacterialMutationPromoterT7- CMV/CAGAvailable sinceJune 22, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCoofy6
Plasmid#43990DepositorTypeEmpty backboneUseTagsHis6-Sumo3ExpressionBacterialMutationPromoterT7Available sinceOct. 28, 2013AvailabilityAcademic Institutions and Nonprofits only -
pACEC3GH (Apr)
Plasmid#110085PurposeReplicative, acetamide-based expression vector containing a C-terminal 3C cleavage site and a GPF-fusion with a His10-tag. Suitable for high-level expression levels amenable for large scale purification of proteins.DepositorTypeEmpty backboneUseTags3C cleavage site - GFP - His10 TagExpressionBacterialMutationPromoterAvailable sinceJune 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
pGBW-m4046763
Plasmid#145606PurposeBacterial Expression plasmid for SARS-CoV-2 M (membrane)DepositorInsertSARS-CoV-2 M (membrane) (M Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
UseTagsCleavable TEV;FLAG_2ExpressionBacterialMutationwtPromoterPromoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…Available sinceApril 29, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pET-32a(+)-TGFB3m
Plasmid#120289PurposeProduce human recombinant mTGFB3 in DE3 cellsDepositorInsertmonomeric Transforming Growth Factor-β3
UseTags6x His and S-TagExpressionBacterialMutationCodon optimized for E. Coli productionPromoterT7 PromoterAvailable sinceJune 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
pGEX SHP-1(C)-SH2
Plasmid#46494DepositorInsertSHP-1(C) (PTPN6 Human)
UseTagsGST and PreScissionExpressionBacterialMutationcontains amino acids 105-217PromoterTacAvailable sinceJuly 10, 2013AvailabilityAcademic Institutions and Nonprofits only
