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Addgene
Showing: 9261 - 9280 of 27133 results

  1. pDA186

    Plasmid
    #74250
    Purpose
    Intermediate cloning vector, with MCS2 containing inducible unstable peptide driven by pGPD1 (UbiY 2xNLSs SZ3).
    Depositor
    Serge Pelet
    Article
    Aymoz et al Nat Commun. 2016 Apr 21;7:11304. doi: 10
    Insert
    Inducible peptide of the dPSTR
    Use
    Tags
    Expression
    Bacterial and Yeast
    Mutation
    Promoter
    pGPD1
    Availability
    Academic Institutions and Nonprofits only
    74250-plasmid-map-sequence-id-139759

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  2. pDA171

    Plasmid
    #74251
    Purpose
    Intermediate cloning vector, with MCS2 containing inducible stable peptide driven by pSTL1 (Venus 2xNLSs SZ1).
    Depositor
    Serge Pelet
    Article
    Aymoz et al Nat Commun. 2016 Apr 21;7:11304. doi: 10
    Insert
    Inducible peptide of the dPSTR
    Use
    Tags
    Venus
    Expression
    Bacterial and Yeast
    Mutation
    Promoter
    pSTL1
    Availability
    Academic Institutions and Nonprofits only
    74251-plasmid-map-sequence-id-139758

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  4. pJK556

    Plasmid
    #74277
    Purpose
    Produces formylglycinamide ribonucleotide amidotransferase, N-terminal His6 tag (H6EcPurL)
    Depositor
    T. J. Kappock
    Insert
    formylglycinamidine ribonucleotide synthetase
    Use
    Tags
    His6
    Expression
    Bacterial
    Mutation
    changes alanine-1246 to serine, numerous silent m…
    Promoter
    T7
    Availability
    Academic Institutions and Nonprofits only
    74277-plasmid-map-sequence-id-139780

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  7. pJK591

    Plasmid
    #71589
    Purpose
    Produces Acetobacter aceti 1023 acetyl-CoA:oxalate CoA-transferase (UctC) with Thr2>Ala/Asp177>Ala mutants (UctC-T2A,D177A)
    Depositor
    T. J. Kappock
    Article
    Mullins et al PLoS One. 2013 Jul 23;8(7):e67901. doi:
    Insert
    acetyl-CoA:oxalate CoA-transferase
    Use
    Tags
    Expression
    Bacterial
    Mutation
    changes threonine-2 to alanine and aspartate-177 …
    Promoter
    T7
    Availability
    Academic Institutions and Nonprofits only
    71589-plasmid-map-sequence-id-130927

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  9. pJK511

    Plasmid
    #71591
    Purpose
    Produces Thermoplasma acidophilum citrate synthase with C-terminal His6 tag, His222>Gln mutant (TpCSH6-H222Q)
    Depositor
    T. J. Kappock
    Article
    Murphy et al Acta Crystallogr F Struct Biol Commun. 2
    Insert
    citrate synthase
    Use
    Tags
    His6
    Expression
    Bacterial
    Mutation
    changes histidine-222 to glutamine
    Promoter
    T7 promoter
    Availability
    Academic Institutions and Nonprofits only
    71591-plasmid-map-sequence-id-130911

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  10. pJK505

    Plasmid
    #71594
    Purpose
    Produces Acetobacter aceti 1023 N5-carboxyaminoimidazole ribonucleotide mutase, Trp34>Phe mutant (AaPurE1fww)
    Depositor
    T. J. Kappock
    Insert
    N5-carboxyaminoimidazole ribonucleotide mutase
    Use
    Tags
    Expression
    Bacterial
    Mutation
    changes tryptophan-34 to phenylalanine, additiona…
    Promoter
    T7
    Availability
    Academic Institutions and Nonprofits only
    71594-plasmid-map-sequence-id-131669

    Enlarge

  11. pJK506

    Plasmid
    #71596
    Purpose
    Produces Acetobacter aceti 1023 N5-carboxyaminoimidazole ribonucleotide mutase, Trp165>Phe mutant (AaPurE1wwf)
    Depositor
    T. J. Kappock
    Insert
    N5-carboxyaminoimidazole ribonucleotide mutase
    Use
    Tags
    Expression
    Bacterial
    Mutation
    changes tryptophan-165 to phenylalanine, addition…
    Promoter
    T7
    Availability
    Academic Institutions and Nonprofits only
    71596-plasmid-map-sequence-id-131670

    Enlarge

  12. pJK507

    Plasmid
    #71597
    Purpose
    Produces Acetobacter aceti 1023 N5-carboxyaminoimidazole ribonucleotide mutase, Trp34>Phe/Trp165>Phe mutant (AaPurE1fwf)
    Depositor
    T. J. Kappock
    Insert
    N5-carboxyaminoimidazole ribonucleotide mutase
    Use
    Tags
    Expression
    Bacterial
    Mutation
    changes tryptophan-34 to phenylalanine, tryptopha…
    Promoter
    T7
    Availability
    Academic Institutions and Nonprofits only
    71597-plasmid-map-sequence-id-131671

    Enlarge

  13. pJK508

    Plasmid
    #71598
    Purpose
    Produces Acetobacter aceti 1023 N5-carboxyaminoimidazole ribonucleotide mutase, Trp34>Phe/His59>Asn/Trp165>Phe mutant (AaPurE1fwf-H59N)
    Depositor
    T. J. Kappock
    Insert
    N5-carboxyaminoimidazole ribonucleotide mutase
    Use
    Tags
    Expression
    Bacterial
    Mutation
    changes tryptophan-34 to phenylalanine, histidine…
    Promoter
    T7
    Availability
    Academic Institutions and Nonprofits only
    71598-plasmid-map-sequence-id-131672

    Enlarge

  14. pJK509

    Plasmid
    #71599
    Purpose
    Produces Acetobacter aceti 1023 N5-carboxyaminoimidazole ribonucleotide mutase, Trp34>Phe/His59>Asn mutant (AaPurE1fww-H59N)
    Depositor
    T. J. Kappock
    Insert
    N5-carboxyaminoimidazole ribonucleotide mutase
    Use
    Tags
    Expression
    Bacterial
    Mutation
    changes tryptophan-34 to phenylalanine, histidine…
    Promoter
    T7
    Availability
    Academic Institutions and Nonprofits only
    71599-plasmid-map-sequence-id-131673

    Enlarge

  15. pJK510

    Plasmid
    #71600
    Purpose
    Produces Acetobacter aceti 1023 N5-carboxyaminoimidazole ribonucleotide mutase, His59>Asn/Trp165>Phe mutant (AaPurE1wwf-H59N)
    Depositor
    T. J. Kappock
    Insert
    N5-carboxyaminoimidazole ribonucleotide mutase
    Use
    Tags
    Expression
    Bacterial
    Mutation
    changes histidine-59 to asparagine, tryptophan-16…
    Promoter
    T7
    Availability
    Academic Institutions and Nonprofits only
    71600-plasmid-map-sequence-id-131674

    Enlarge

  16. pJK525

    Plasmid
    #71601
    Purpose
    Produces Acetobacter aceti 1023 N5-carboxyaminoimidazole ribonucleotide mutase, Trp34>Phe/Trp97>Tyr mutant (AaPurE1fyw)
    Depositor
    T. J. Kappock
    Insert
    N5-carboxyaminoimidazole ribonucleotide mutase
    Use
    Tags
    Expression
    Bacterial
    Mutation
    changes tryptophan-34 to phenylalanine, tryptopha…
    Promoter
    T7
    Availability
    Academic Institutions and Nonprofits only
    71601-plasmid-map-sequence-id-131675

    Enlarge

  17. pJK526

    Plasmid
    #71602
    Purpose
    Produces Acetobacter aceti 1023 N5-carboxyaminoimidazole ribonucleotide mutase, Trp97>Tyr/Trp165>Phe mutant (AaPurE1wyf)
    Depositor
    T. J. Kappock
    Insert
    N5-carboxyaminoimidazole ribonucleotide mutase
    Use
    Tags
    Expression
    Bacterial
    Mutation
    changes tryptophan-97 to tyrosine, tryptophan-165…
    Promoter
    T7
    Availability
    Academic Institutions and Nonprofits only
    71602-plasmid-map-sequence-id-131676

    Enlarge

  18. pJK559

    Plasmid
    #71603
    Purpose
    Produces Acetobacter aceti 1023 N5-carboxyaminoimidazole ribonucleotide mutase, Trp34>Phe/Tyr154>Phe/Trp165>Phe mutant (AaPurE1fwf-Y154F)
    Depositor
    T. J. Kappock
    Insert
    N5-carboxyaminoimidazole ribonucleotide mutase
    Use
    Tags
    Expression
    Bacterial
    Mutation
    changes tryptophan-34 to phenylalanine, tyrosine-…
    Promoter
    T7
    Availability
    Academic Institutions and Nonprofits only
    71603-plasmid-map-sequence-id-131677

    Enlarge

  19. pJK562

    Plasmid
    #71604
    Purpose
    Produces Acetobacter aceti 1023 N5-carboxyaminoimidazole ribonucleotide mutase, Trp34>Phe/Tyr154>Leu/Trp165>Phe mutant (AaPurE1fwf-Y154L)
    Depositor
    T. J. Kappock
    Insert
    N5-carboxyaminoimidazole ribonucleotide mutase
    Use
    Tags
    Expression
    Bacterial
    Mutation
    changes tryptophan-34 to phenylalanine, tyrosine-…
    Promoter
    T7
    Availability
    Academic Institutions and Nonprofits only
    71604-plasmid-map-sequence-id-131680

    Enlarge

  20. pJK600

    Plasmid
    #71605
    Purpose
    Produces Acetobacter aceti 1023 N5-carboxyaminoimidazole ribonucleotide mutase, Trp34>Phe/His59>Asn/Tyr154>Phe/Trp165>Phe mutant (AaPurE1fwf-H59N,Y154F)
    Depositor
    T. J. Kappock
    Insert
    N5-carboxyaminoimidazole ribonucleotide mutase
    Use
    Tags
    Expression
    Bacterial
    Mutation
    changes tryptophan-34 to phenylalanine, histidine…
    Promoter
    T7
    Availability
    Academic Institutions and Nonprofits only
    71605-plasmid-map-sequence-id-131682

    Enlarge

Showing: 9261 - 9280 of 27133 results