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Plasmid#140839Purposewild-type Halothiobacillus neapolitanus Form I Rubisco with StrepII tag (CbbL-StrepII, CbbS), pET-14b backboneDepositorInsertForm IA Rubisco; CbbL, CbbS
UseTagsStrep IIExpressionBacterialMutationPromoterT7Available sinceJune 22, 2020AvailabilityAcademic Institutions and Nonprofits only -
Ubiquitin G76C
Plasmid#86583PurposeBacterial expression of Ubiquitin G76C for cross linking studiesDepositorInsertUbiquitin (UBC Human)
UseTagsHis6-MBPExpressionBacterialMutationTEV site; G76CPromoterTacAvailable sinceFeb. 24, 2017AvailabilityIndustry, Academic Institutions, and Nonprofits -
pHH0103_VCP_AAA
Plasmid#109964PurposeProtein expression and purification of VCP_AAADepositorInsertVCP_AAA (VCP Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceNov. 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
pETSXM2-pLacI-mtrC
Plasmid#174615PurposeBacterial expression of mtrC from Shewanella oneidensis MR-1.DepositorInsertMtrC
UseSynthetic Biology; Shuttle vector in e. coli and …TagsExpressionBacterialMutationPromoterpLacIAvailable sinceOct. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET21a.TM0857
Plasmid#11419DepositorInsertflavin binding protein (TM0857 T. maritima)
UseTagsExpressionBacterialMutationPromoterAvailable sinceApril 10, 2006AvailabilityAcademic Institutions and Nonprofits only -
pGBW-m4046871
Plasmid#145724PurposeBacterial Expression plasmid for SARS-CoV-2 nsp10DepositorInsertSARS-CoV-2 nsp10 (ORF1ab Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
UseTagsCleavable TEV;6xHISExpressionBacterialMutationEscherichia coli recode 1PromoterPromoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…Available sinceApril 27, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pET28a SV40 ST full length
Plasmid#132636Purposeexpresses SV40 ST full length in E. coliDepositorInsertSV40 small t antigen
UseTagsExpressionBacterialMutationPromoterAvailable sinceNov. 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
DinoIII-gfp
Plasmid#128141PurposeDinoflagelate backbone for gfp expressionDepositorInsertGreen fluorescence protein
UseTagsExpressionBacterialMutationPromoterDinoflagellate potential promotersAvailable sinceApril 29, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAV10.K5
Plasmid#63210PurposepCACS backbone (Amp), LEU2, contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for TU assembly using yGG. Integration into YKL162C-A (NotI or BciVI).DepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionBacterial and YeastMutationPromoterAvailable sinceFeb. 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAV10.K4
Plasmid#63212PurposepCACS backbone (Amp), TRP1, contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for TU assembly using yGG. Integration into chrIXR: 387328-388330.DepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionBacterial and YeastMutationPromoterAvailable sinceFeb. 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
pNP1 eforRed trigger
Plasmid#107357PurposeTrigger sequence for pNP1 eforRed toehold switchDepositorInserttoehold trigger 1
UseTagsExpressionBacterialMutationPromoterT7Available sinceJan. 22, 2019AvailabilityAcademic Institutions and Nonprofits only -
pETM6-P.accABCD
Plasmid#66866PurposeEncodes accA, accB, accC, accD in Pseudo-operon configuration.DepositorInsertsaccA
accB
accC
accD
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterT7Available sinceJuly 9, 2015AvailabilityAcademic Institutions and Nonprofits only -
pJL1-PD-4xDQNAT
Plasmid#128391PurposeIn vitro expression of H. influenzae protein D with C terminal 4xDQNAT glycosylation sitesDepositorInsertProtein D
UseTags4xDQNAT glycosylation tag and 6xHis tagExpressionBacterialMutationPromoterT7Available sinceApril 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
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CoV-2-NSP3-c002
Plasmid#173084PurposeExpression of recombinant fusion protein in E. coliDepositorInsertrep (ORF1ab Severe acute respiratory syndrome coronavirus 2 (2019-nCoV) (SARS-CoV-2))
UseTagsExpressionBacterialMutationE1084-E1192, codon optimizedPromoterAvailable sinceDec. 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
Ki67 (8 repeats+LR)-mNeonGreen-IRESpuro2
Plasmid#183741PurposeExpresses Ki67 (8 repeats+LR)-mNeonGreenDepositorInsertMKI67 LR domain + 8 Ki-67 repeats (MKI67 Human)
UseTagsmNeonGreenExpressionBacterial and MammalianMutationMKI67 LR domain + 8 Ki-67 repeatsPromoterCMVAvailable sinceJuly 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTE1059
Plasmid#186628PurposeExpression of S. coelicolor butyrolactone biosynthesis genes in E. coli. Heterologous production of SCBs.DepositorInsertsscbA
scbB
scbC
UseSynthetic BiologyTags6xHis and 6xHis-thrombinExpressionBacterialMutationPromoterSynthetic promoter B10, Synthetic promoter B19, a…Available sinceSept. 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSW002-Pc-TorA(sp)
Plasmid#111253PurposeBroad host-range bacterial expression vector with constitutive Pc promoter followed by the E. coli TorA signal peptideDepositorInsertTorA signal peptide
UseTagsExpressionBacterialMutationPromoterPcAvailable sinceJuly 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
MBP-RGG-SmBit-RGG
Plasmid#176644PurposeSmBit fused to LAF-1 RGG domain with N-terminal MBP tagDepositorInsertMBP-RGG-SmBit-RGG
UseTagsExpressionBacterialMutationPromoterAvailable sinceOct. 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBEST-p15a-OR2-OR1-Pr-UTR1-sigma28-T500
Plasmid#45779DepositorInsertsigma factor 28
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterOR2-OR1-Pr (bacteriophage Lambda with one mutatio…Available sinceJuly 19, 2013AvailabilityAcademic Institutions and Nonprofits only