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JBEI-12050

Plasmid

#84982

Purpose

Isopentenol production

Depositor

Taek Soon Lee

Article

Kang et al Metab Eng. 2016 Mar;34:25-35. doi: 10.10

Insert

MevTo-T21-MK-aphA

Use

Tags

Expression

Bacterial

Mutation

Promoter

LacUV5

Available since

July 31, 2017

Availability

Academic Institutions and Nonprofits only

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pET14b_tev_PSGU

Plasmid

#59738

Purpose

Expresses a.a. 417-887 of human ZO-1

Depositor

Alan Fanning

Article

Nomme et al J Biol Chem. 2011 Dec 16;286(50):43352-6

Insert

zonula occludens-1 (TJP1 Human)

Use

Tags

HIS tag and thrombin clvg

Expression

Bacterial

Mutation

Promoter

T7

Available since

Nov. 5, 2014

Availability

Academic Institutions and Nonprofits only
pSB_init_Sb68

Plasmid

#159423

Purpose

Expression of the anti-TGP (thermostable GFP) synthetic nanobody Sb68

Depositor

Dianfan Li

Article

Cai et al Commun Biol. 2020 Dec 10;3(1):753. doi:

Insert

Sb68

Use

Tags

c-Myc and poly-His

Expression

Bacterial

Mutation

Promoter

Available since

Jan. 19, 2021

Availability

Academic Institutions and Nonprofits only

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pHH0103_LYN-1/1

Plasmid

#91536

Purpose

Protein expression and purification of human SH3 domain construct LYN-1/1

Depositor

Sachdev Sidhu

Article

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

LYN-1/1 (LYN Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

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pBluescriptII[attB/Ins1]

Plasmid

#74100

Purpose

"intermediate vector" for the two-step cloning method (Wang and Szaro, Dev Biol, 2015)

Depositor

Ben Szaro

Article

Wang et al Dev Biol. 2015 Feb 1;398(1):11-23. doi:

Inserts

Phi C31 attb element
Dual chicken beta-globin hypersensitive site 4 (HS4) insulators

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

May 26, 2016

Availability

Academic Institutions and Nonprofits only

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pBM3R1-B1

Plasmid

#74678

Purpose

BM3R1-B1 gate

Depositor

Christopher Voigt

Article

Nielsen et al Science. 2016 Apr 1;352(6281):aac7341. d

Insert

PTac-RBSB1-BM3R1-PBM3R1-YFP

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

April 22, 2016

Availability

Academic Institutions and Nonprofits only

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p4020 pGEM HPV-18 E6

Plasmid

#10856

Depositor

Peter Howley

Article

Werness et al Science. 1990 Apr 6. 248(4951):76-9.

Insert

HPV18 E6 (E6 Human papillomavirus)

Use

Tags

HA?

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 26, 2005

Availability

Academic Institutions and Nonprofits only
pMRE-Tn7-153

Plasmid

#118567

Purpose

miniTn7 plasmid to deliver constitutively expressed fluorescent protein genes in bacteria

Depositor

Mitja Remus-Emsermann

Article

Schlechter et al Front Microbiol. 2018 Dec 12;9:3052. doi

Insert

sYFP2

Use

Minitn7 delivery vector

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 29, 2018

Availability

Academic Institutions and Nonprofits only

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pSEL1

Plasmid

#23011

Depositor

James Bowie

Article

Massey-Gendel et al Protein Sci. 2009 Feb . 18(2):372-83.

Insert

DHFR (Dhfr Mouse)

Use

Tags

6 His Tag (not in frame) and Xpress epitope (not …

Expression

Bacterial

Mutation

Promoter

Available since

March 10, 2010

Availability

Academic Institutions and Nonprofits only
pHis-hMTMR1

Plasmid

#100723

Purpose

Expression of human MTMR1 PH-PTP domain

Depositor

Byung Il Lee

Article

Bong et al PLoS One. 2016 Mar 28;11(3):e0152611. do

Insert

MTMR1 (MTMR1 Human)

Use

Tags

His

Expression

Bacterial

Mutation

Contains amino acids 95-607

Promoter

T7

Available since

Nov. 9, 2017

Availability

Academic Institutions and Nonprofits only

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pNIC28-SENP2

Plasmid

#196355

Purpose

protein expression of the His-tagged catalytic domain

Depositor

Stefan Knapp

Insert

SENP2 catalytic domain

Use

Tags

His6-TEV

Expression

Bacterial

Mutation

contains only D364-L589

Promoter

T7

Available since

March 28, 2023

Availability

Academic Institutions and Nonprofits only

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PTPN7

Plasmid

#38945

Purpose

Bacterial expression for structure determination; may not be full ORF

Depositor

Nicola Burgess-Brown

Insert

PTPN7 (PTPN7 Human)

Use

Tags

His Tev

Expression

Bacterial

Mutation

Promoter

Available since

March 18, 2013

Availability

Academic Institutions and Nonprofits only
H9 pMalLP

Plasmid

#41811

Depositor

Chris Garcia

Article

Levin et al Nature. 2012 Mar 25;484(7395):529-33. do

Insert

H9 Super-2 (IL2 Human)

Use

Tags

3C protease site, 6x His, and Maltose Binding Pro…

Expression

Bacterial

Mutation

Promoter

pTac

Available since

Jan. 8, 2013

Availability

Academic Institutions and Nonprofits only
pIntegrase_13

Plasmid

#60584

Purpose

plasmid contains the integrase gene under the control of the arabinose-inducible promoter, Pbad

Depositor

Christopher Voigt

Article

Yang et al Nat Methods. 2014 Dec;11(12):1261-6. doi

Insert

Integrase_13

Use

Tags

Expression

Bacterial

Mutation

Codon optimized for E.coli

Promoter

pBAD

Available since

March 2, 2015

Availability

Academic Institutions and Nonprofits only

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pUC19/DdmEGFP

Plasmid

#154304

Purpose

cloned with the codon-optimized mEGFP gene for expression in Dictyostelium discoideum

Depositor

Shigehiko Yumura

Article

Kondo et al Biotechniques. 2020 Feb;68(2):91-95. doi

Insert

mEGFP

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Aug. 3, 2020

Availability

Academic Institutions and Nonprofits only

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pGR-BBa_B0010

Plasmid

#46003

Depositor

Christopher Voigt

Article

Chen et al Nat Methods. 2013 Jun 2. doi: 10.1038/nm

Insert

BBa_B0010 Terminator

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

PBAD

Available since

June 28, 2013

Availability

Academic Institutions and Nonprofits only

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pBW400hrpL-gfp

Plasmid

#61437

Purpose

pBW400hrpL-gfp

Depositor

Baojun Wang, Martin Buck

Article

Wang et al Nat Commun. 2011 Oct 18;2:508. doi: 10.1

Insert

hrpL-rbs30-gfp-ter

Use

Tags

Expression

Bacterial

Mutation

Promoter

HrpL

Available since

Aug. 14, 2015

Availability

Academic Institutions and Nonprofits only

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pT2-cryR;dll4P1Egfp

Plasmid

#90160

Purpose

contains dll4 specific gene promoter driving expression of eGFP

Depositor

Nathan Lawson

Article

Quillien et al Cell Rep. 2017 Jul 18;20(3):709-720. doi

Insert

dll4P1Egfp (dll4 Zebrafish)

Use

Tags

EGFP

Expression

Bacterial

Mutation

Promoter

Available since

May 24, 2017

Availability

Academic Institutions and Nonprofits only

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pCAH01Sp::Cas9D10A

Plasmid

#128161

Purpose

Ptet-controlled expression of S. pyogenes Cas9D10A variant

Depositor

Calvin Henard

Article

Tapscott et al Appl Environ Microbiol. 2019 May 16;85(1

Insert

Sp Cas9

Use

Tags

Expression

Bacterial

Mutation

D10A

Promoter

Ptet

Available since

July 15, 2019

Availability

Academic Institutions and Nonprofits only

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pBEST-p15A-PLtetO1-tetR-T500

Plasmid

#45391

Depositor

Vincent Noireaux

Article

Shin et al J Biol Eng. 2010 Jun 24;4:8.

Insert

tet repressor

Use

Tags

Expression

Bacterial

Mutation

Promoter

PL-tetO1

Available since

June 13, 2013

Availability

Academic Institutions and Nonprofits only

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