-
Plasmid#28013DepositorInsertEYFP-fuse, T7 RNAP
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 21, 2011AvailabilityAcademic Institutions and Nonprofits only -
pBAD/HisB (TIREVOL)-mEos3.2
Plasmid#189720Purposetitratable expression of mEos3.2 in bacteriaDepositorInsertmEos3.2
UseTagsHis-T7-EKExpressionBacterialMutationPromoterpBADAvailable sinceOct. 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET21-pelB-LaG-17
Plasmid#172747PurposeBacterial expression of HIS-tagged anti-GFP nanobody LaG-17; HIS tag preceded by HRV 3C/PreScission cleavage site.DepositorInsertLaG-17
UseTags6xHIS and pelB leader for periplasmic secretionExpressionBacterialMutationPromoterT7Available sinceAug. 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
pTSlb-wt
Plasmid#60728PurposeExpresses both fragments of T7 RNAP split at position 179. The N-terminal fragment is driven by PLac while the C-terminal fragment is driven by PBAD.DepositorInsertsResidues 1-179 of split T7 RNAP
Residues 180-880 of split T7 RNAP
UseSynthetic BiologyTagsExpressionBacterialMutationResidues 1-180 of split T7 RNAP and Residues 180-…PromoterAvailable sinceNov. 4, 2014AvailabilityAcademic Institutions and Nonprofits only -
pET28a-his-Gluc-iLID
Plasmid#172096PurposeExpresses the Gluc-iLID fusion protein in bacteriaDepositorInsertGaussia Luciferase - iLID
UseTagsExpressionBacterialMutationGluc Mutations - M43L, M110LPromoterT7Available sinceApril 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET-DUET-Prp18
Plasmid#111354Purposeprotein purificationDepositorInsertPRP18
UseTagsExpressionBacterialMutationPromoterAvailable sinceJuly 31, 2018AvailabilityAcademic Institutions and Nonprofits only -
JBEI-12050
Plasmid#84982PurposeIsopentenol productionDepositorInsertMevTo-T21-MK-aphA
UseTagsExpressionBacterialMutationPromoterLacUV5Available sinceJuly 31, 2017AvailabilityAcademic Institutions and Nonprofits only -
pET14b_tev_PSGU
Plasmid#59738PurposeExpresses a.a. 417-887 of human ZO-1DepositorInsertzonula occludens-1 (TJP1 Human)
UseTagsHIS tag and thrombin clvgExpressionBacterialMutationPromoterT7Available sinceNov. 5, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCMV-EPN-05
Plasmid#79920PurposeMyr4-12GS-I3-01-myc-Late1DepositorInsertEPN-05
UseTagsExpressionBacterial and MammalianMutationPromoterAvailable sinceFeb. 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCMV-EPN-10
Plasmid#79925PurposePalm4-12GS-I3-01-myc-Late1DepositorInsertEPN-10
UseTagsExpressionBacterial and MammalianMutationPromoterAvailable sinceFeb. 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
pGEX-4T-1 EGFP-Sumo2
Plasmid#13385DepositorInsertSumo2 (SUMO2 Human)
UseTagsEGFP and GSTExpressionBacterialMutationPromoterAvailable sinceNov. 16, 2006AvailabilityAcademic Institutions and Nonprofits only -
pAC1750
Plasmid#127984PurposeAMA1 plasmid with Aspergillus optimized Cpf1, ble selection marker, and USER cassette (PacI/Nt.BbvCI)DepositorInsertCpf1
UseCRISPR; Fungal expressionTagsSV40 NLSExpressionBacterialMutationcodon optimized for Aspergillus nidulansPromoterAspergillus nidulans tef1 promoterAvailable sinceAug. 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
pSB_init_Sb68
Plasmid#159423PurposeExpression of the anti-TGP (thermostable GFP) synthetic nanobody Sb68DepositorInsertSb68
UseTagsc-Myc and poly-HisExpressionBacterialMutationPromoterAvailable sinceJan. 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
pHH0103_LYN-1/1
Plasmid#91536PurposeProtein expression and purification of human SH3 domain construct LYN-1/1DepositorInsertLYN-1/1 (LYN Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
YSCD44A-c002
Plasmid#175493PurposeProtein expression in bacterial cells. Single chain Fv antibody to human CD44 Link domain. Can be used for crystallography.DepositorInsertSingle chain Fv CD44 antibody (H90)
UseTags3XFLAG-EK-His8 and pelBExpressionBacterialMutationPromoterAvailable sinceJan. 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
pT5T_hp53_ST
Plasmid#34922DepositorInsertp53 DNA-binding (Res 1-393) (TP53 Human)
UseTagsExpressionBacterialMutationC135V, C141V, W146Y, C182S, V203A, R209P, C229Y, …PromoterT7Available sinceFeb. 22, 2012AvailabilityAcademic Institutions and Nonprofits only -
pBAD_His B_QuasAr2
Plasmid#64134PurposeFluorescent reporter of membrane voltage with improved sensitivityDepositorInsertQuasAr2
UseTagsExpressionBacterialMutationPromoterAvailable sinceAug. 20, 2015AvailabilityAcademic Institutions and Nonprofits only -
pBM3R1-B1
Plasmid#74678PurposeBM3R1-B1 gateDepositorInsertPTac-RBSB1-BM3R1-PBM3R1-YFP
UseTagsExpressionBacterialMutationPromoterAvailable sinceApril 22, 2016AvailabilityAcademic Institutions and Nonprofits only -
p4020 pGEM HPV-18 E6
Plasmid#10856DepositorInsertHPV18 E6 (E6 Human papillomavirus)
UseTagsHA?ExpressionBacterialMutationPromoterAvailable sinceOct. 26, 2005AvailabilityAcademic Institutions and Nonprofits only -
pBluescriptII[attB/Ins1]
Plasmid#74100Purpose"intermediate vector" for the two-step cloning method (Wang and Szaro, Dev Biol, 2015)DepositorInsertsPhi C31 attb element
Dual chicken beta-globin hypersensitive site 4 (HS4) insulators
UseTagsExpressionBacterialMutationPromoterAvailable sinceMay 26, 2016AvailabilityAcademic Institutions and Nonprofits only