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pAT892

Plasmid

#68933

Purpose

Int9 expressed constitutively from PAM4 and the rpiL* RBS, pNBU2 backbone, AmpR

Depositor

Timothy Lu, Christopher Voigt

Article

Mimee et al Cell Syst. 2015 Jul 29;1(1):62-71. doi:

Insert

Int9

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 2, 2015

Availability

Academic Institutions and Nonprofits only

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pET-45b-mEGFP-HMGB1-K184Rfs

Plasmid

#194544

Purpose

Bacterial Expression of 6xHis mEGFP-HMGB1 Mutant (K184Rfs*44) Fusion Protein

Depositor

Denes Hnisz

Article

Mensah et al Nature. 2023 Feb;614(7948):564-571. doi:

Insert

HMGB1 (HMGB1 Human)

Use

Tags

mEGFP

Expression

Bacterial

Mutation

K184Rfs*44

Promoter

Available since

Feb. 23, 2023

Availability

Academic Institutions and Nonprofits only

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tIVR

Plasmid

#122663

Purpose

Express tIVR protein in E. coli

Depositor

Robert Cohen

Article

Choi et al Nat Methods. 2019 Jul 15. pii: 10.1038/s

Insert

tIVR

Use

Tags

His-tag

Expression

Bacterial

Mutation

Promoter

T7 promoter

Available since

July 17, 2019

Availability

Industry, Academic Institutions, and Nonprofits

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pSK-c-mpl

Plasmid

#1828

Depositor

Philip Leder

Article

Skoda et al EMBO J 1993 Jul;12(7):2645-53.

Insert

c-mpl (Mpl Mouse)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

May 25, 2005

Availability

Academic Institutions and Nonprofits only
pCoofy22

Plasmid

#43992

Depositor

Sabine Suppmann

Article

Scholz et al BMC Biotechnol. 2013 Feb 14;13(1):12.

Type

Empty backbone

Use

Tags

His10 and S

Expression

Bacterial

Mutation

Promoter

T7

Available since

Oct. 28, 2013

Availability

Academic Institutions and Nonprofits only

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pEDJ400

Plasmid

#177269

Purpose

USER cloning-compatible 2μ vector (URA3)

Depositor

Michael Jensen

Article

Jensen et al Nucleic Acids Res. 2021 Sep 7;49(15):e88

Type

Empty backbone

Use

Tags

Expression

Bacterial and Yeast

Mutation

Promoter

Available since

Jan. 26, 2022

Availability

Academic Institutions and Nonprofits only

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pEDJ333

Plasmid

#177272

Purpose

GAL1p:Cas9:CYC1t

Depositor

Michael Jensen

Article

Jensen et al Nucleic Acids Res. 2021 Sep 7;49(15):e88

Insert

Cas9

Use

Tags

Expression

Bacterial and Yeast

Mutation

Promoter

GAL1

Available since

Dec. 8, 2021

Availability

Academic Institutions and Nonprofits only

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pMA-Thermal_CTLA-4

Plasmid

#199751

Purpose

Thermal expression of CTLA-4

Depositor

Mikhail Shapiro

Article

Abedi et al Nat Commun. 2022 Mar 24;13(1):1585. doi:

Insert

CTLA-4 (Ctla4 Mouse)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

May 17, 2023

Availability

Academic Institutions and Nonprofits only

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pMTTH+H1.4ΔCTD

Plasmid

#157797

Purpose

Expression of human linker histone H1.4 without c-terminal tail

Depositor

Michael Rosen

Article

Gibson et al Cell. 2019 Oct 3;179(2):470-484.e21. doi

Insert

H1.4ΔCTD (H1-4 Human)

Use

Tags

His6 and MBP

Expression

Bacterial

Mutation

Promoter

pTAC

Available since

Aug. 5, 2020

Availability

Academic Institutions and Nonprofits only

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pET-28a+GFP-CAMSAP2 CKK

Plasmid

#59032

Purpose

expresses CKK domain of CAMSAP2 in E. coli

Depositor

Ron Vale

Article

Hendershott et al Proc Natl Acad Sci U S A. 2014 Apr 22;11

Insert

CAMSAP2 CKK domain (CAMSAP2 Human)

Use

Tags

His, Strep, TEV, and sfGFP

Expression

Bacterial

Mutation

Promoter

Available since

Aug. 27, 2014

Availability

Academic Institutions and Nonprofits only
pKEW336-MBP-TEV-MbCas12a-33362

Plasmid

#115670

Purpose

Expresses MbCas12 (from M. bovoculi 33362) in bacteria via T7 RNAP, for purification

Depositor

Jennifer Doudna

Article

Watters et al Science. 2018 Oct 12;362(6411):236-239.

Insert

MbCas12a

Use

CRISPR

Tags

MBP-TEV

Expression

Bacterial

Mutation

Promoter

T7 RNAP

Available since

Oct. 25, 2018

Availability

Academic Institutions and Nonprofits only

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Phat2-hs-cPla2-C2-EGFP-GH

Plasmid

#187088

Purpose

Protein Expression

Depositor

Philipp Niethammer

Article

Shen et al Proc Natl Acad Sci U S A. 2022 Jan 4;119

Insert

ENSG00000116711 (PLA2G4A Human)

Use

Tags

EGFP

Expression

Bacterial

Mutation

Promoter

Available since

Aug. 3, 2022

Availability

Academic Institutions and Nonprofits only

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pAWP78-PVCpnf_pvc13-MoonTag

Plasmid

#198299

Purpose

Produces PVCs retargeted with MoonTag

Depositor

Feng Zhang

Article

Kreitz et al Nature. 2023 Apr;616(7956):357-364. doi:

Insert

PVCpnf_pvc13-MoonTag

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 29, 2023

Availability

Academic Institutions and Nonprofits only

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pSrpR-S4

Plasmid

#74694

Purpose

SrpR-S4 gate

Depositor

Christopher Voigt

Article

Nielsen et al Science. 2016 Apr 1;352(6281):aac7341. d

Insert

PTac-RBSS4-SrpR-PSrpR-YFP

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

April 29, 2016

Availability

Academic Institutions and Nonprofits only

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pBM3R1-B3

Plasmid

#74680

Purpose

BM3R1-B3 gate

Depositor

Christopher Voigt

Article

Nielsen et al Science. 2016 Apr 1;352(6281):aac7341. d

Insert

PTac-RBSB3-BM3R1-PBM3R1-YFP

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

April 22, 2016

Availability

Academic Institutions and Nonprofits only

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pMK-AttL1-NotI/BamHI-U6-BsaI-tracrRNAopt-BamHI-U6-SapI-tracrRNAopt-NotI/XbaI-7sk-BsmBI-tracrRNA-XbaI-AttL2

Plasmid

#190898

Purpose

Entry vector for multiple sgRNA cloning

Depositor

Nicole Déglon

Article

Duarte et al Mol Ther Methods Clin Dev. 2023 Mar 27;2

Type

Empty backbone

Use

CRISPR

Tags

Expression

Bacterial and Mammalian

Mutation

Promoter

U6 and 7sk

Available since

March 27, 2023

Availability

Academic Institutions and Nonprofits only

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pJW4.04

Plasmid

#171209

Purpose

bacterial expression of MBP-tagged PGL-3

Depositor

Geraldine Seydoux

Article

Schmidt et al bioRxiv 2020.10.15.340570

Insert

pgl-3 (pgl-3 Nematode)

Use

Tags

MBP

Expression

Bacterial

Mutation

Promoter

Available since

June 23, 2021

Availability

Academic Institutions and Nonprofits only

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CFP-FRBx5-His

Plasmid

#103784

Purpose

expresses His-tagged iPOLYMER component in E. coli for protein purification

Depositor

Takanari Inoue

Article

Nakamura et al Nat Mater. 2017 Nov 6. pii: nmat5006. do

Insert

CFP-FRBx5-His

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7

Available since

Dec. 1, 2017

Availability

Academic Institutions and Nonprofits only

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BRPF1 (3L42)

Plasmid

#25339

Purpose

Bacterial expression for structure determination; may not be full ORF

Depositor

Cheryl Arrowsmith

Insert

BRPF1_P1 (BRPF1 Human)

Use

Tags

His

Expression

Bacterial

Mutation

Promoter

Available since

Aug. 17, 2010

Availability

Industry, Academic Institutions, and Nonprofits
pRSET FLIPE-600n

Plasmid

#13537

Depositor

Wolf Frommer

Article

Okumoto et al Proc Natl Acad Sci U S A. 2005 Jun 14. 1

Insert

FLIP-E WT

Use

Tags

ECFP and Venus

Expression

Bacterial

Mutation

Promoter

Available since

Dec. 15, 2006

Availability

Academic Institutions and Nonprofits only

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