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Plasmid#52346PurposeCEN6/ARS4 plasmid, REV1 promoter>Recoded TP-DNAP1 (Y427A)DepositorInsertRecoded TP-DNAP
UseTagsExpressionBacterial and YeastMutationPromoterREV1Available sinceJune 10, 2014AvailabilityAcademic Institutions and Nonprofits only -
pMEXC3H
Plasmid#110080PurposeAnalogous to pMINTC3H, but containing oriM for episomal replication in mycobacteria.DepositorTypeEmpty backboneUseTags3C cleavage site - His10 TagExpressionBacterialMutationPromoterAvailable sinceJune 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
pFP.R371
Plasmid#138241PurposeExpresses the split mCherry reporter interrupted by the MP-M-DnaB artificially mini-intein (in cis)DepositorInsertsUseSynthetic BiologyTags6xHisExpressionBacterialMutationPromoterP(araBAD) and iPCAvailable sinceMarch 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
pEB1-sfGFP
Plasmid#103983PurposePlasmid encoding sfGFPDepositorInsertsfGFP
UseLow copyTagsExpressionBacterialMutationMutations relative to wild-type GFP (S30R, Y39N, …PromoterproCAvailable sinceDec. 13, 2017AvailabilityAcademic Institutions and Nonprofits only -
pGS775
Plasmid#20169DepositorInsertRuvC
UseTagsExpressionBacterialMutationnonePromoterAvailable sinceApril 1, 2009AvailabilityAcademic Institutions and Nonprofits only -
pET:GACT:GFP
Plasmid#60734PurposeA modified pET28 driving expression of GFP, biobrick part Bba_E0040. No LacO site downstream of PT7. T7 promoter has base pairs 'GACT' between -11 and -8.DepositorInsertGFP
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceNov. 5, 2014AvailabilityAcademic Institutions and Nonprofits only -
pFS_0351_pET30-RT-CRISPR_Fusicatenibacter_saccharivorans_ARRAY_1
Plasmid#116963PurposeExpresses FsRT-Cas1-Cas2 under pT7lac promoter, encodes FsCRISPR array 1, compatible with SENECA acquisition readoutDepositorInsertFusicatenibacter saccharivorans RT-Cas1-Cas2
UseTagsExpressionBacterialMutationPromoterT7Available sinceDec. 4, 2018AvailabilityAcademic Institutions and Nonprofits only -
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pET-51b(+)-cpHalo∆
Plasmid#194659PurposeProtein production of the split HaloTag part cpHalo∆ (cpHaloTag135/156) in bacteriaDepositorInsertcpHalo∆
UseTagsHis-tagExpressionBacterialMutationPromoterT7Available sinceFeb. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pQnl_Pcphy23
Plasmid#191354PurposeClostridium plasmid expressing NanoLuc reporter from moderate, constitutive promoterDepositorInsertPcphy23-NanoLuc
UseTagsExpressionBacterialMutationPromoterPcphy23Available sinceNov. 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
His-TEV-GB1 Leu5Met
Plasmid#128686PurposeExpresses GB1 Leu5Met mutantDepositorInsertspg
UseTagsHisExpressionBacterialMutationPromoterT7Available sinceApril 1, 2022AvailabilityIndustry, Academic Institutions, and Nonprofits -
pJUMP18-cueR_O
Plasmid#127026PurposeBasic Part O- ORF; cueR; transcription factorDepositorInsertPart cueR_o
UseSynthetic BiologyTagsExpressionBacterialMutationNonePromoterAvailable sinceJuly 1, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET28a Cdk2ap1CAN
Plasmid#178032PurposeExpression vector for the purpose of protein purification.DepositorInsertCdk2ap1 (Cdk2ap1 Mouse)
UseNonviralTags6xHIS - Thrombin - MBP- TEV-TRSExpressionBacterialMutationPromoterT7LacIAvailable sinceMarch 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pFLtR5
Plasmid#167505PurposeBroad-Host range expression vector, RSF1010 origin, TetR repressor, PLtetO-1 promoter, mRFP, Gentamicin Resistance Marker.DepositorInsertTetR-RFP
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterPLtetO-1Available sinceMay 28, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGWF1
Plasmid#18856DepositorTypeEmpty backboneUseTagsCFP and VenusExpressionBacterialMutationPromoterAvailable sinceNov. 21, 2008AvailabilityAcademic Institutions and Nonprofits only -
pJMP1363
Plasmid#119279Purposefor stabilizing ICE in the presence of rapI expressionDepositorInsertdcas9
UseTagsExpressionBacterialMutationD10A and H840APromoterAvailable sinceJan. 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMTB2-NLS-BirA-2A-mCherry_Ras
Plasmid#80067PurposeBeta-actin2 promoter driving HA-tagged biotin ligase (BirA) with NLS, a Thosea asigna virus peptide (2A), and membrane-tethered mCherry protein (membCherry); flanked by Tol2 sequencesDepositorInsertBiotin ligase (BirA) with nuclear localization signal (NLS), a Thosea asigna virus peptide (2A), and mCherry protein
UseZebrafish biotaggingTags3x HAExpressionBacterialMutationPromoterBeta-actin2Available sinceOct. 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
His-MBP human SPOP (aa 28-337)
Plasmid#52294Purposebacterial expression of human SPOP (aa 28-337) fused to His-MBPDepositorInsertSPOP (SPOP Human)
UseTagsHis-MBP and TEVExpressionBacterialMutationPromoterT7Available sinceApril 11, 2014AvailabilityAcademic Institutions and Nonprofits only -
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pC239
Plasmid#65953PurposeN2 inverter plasmid for two-strain systemDepositorInsertsAiiA
LacI
UseTagsLAA taggedExpressionBacterialMutationPromoterpCin and pCin*Available sinceAug. 13, 2015AvailabilityAcademic Institutions and Nonprofits only