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pNIC28-BSA4-G-L11

Plasmid

#127830

Purpose

Production of recombinant human ribosomal protein L11

Depositor

Ivanhoe Leung

Article

Correddu et al Sci Rep. 2019 Jun 20;9(1):8884. doi: 10.

Insert

RPL11 (RPL11 Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7

Available since

Aug. 5, 2019

Availability

Academic Institutions and Nonprofits only

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pET16b-Mms2

Plasmid

#18893

Depositor

Cecile Pickart

Article

Raasi et al Methods Mol Biol. 2005 . 301():47-55.

Insert

Yeast ubiquitin conjugating enzyme Mms2 (MMS2 Budding Yeast)

Use

Tags

HIS10

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 4, 2008

Availability

Academic Institutions and Nonprofits only
pWA PAS-E BAD K-GAFm

Plasmid

#39866

Depositor

Vladislav Verkhusha

Article

Filonov et al Chem Biol. 2013 Jul 23. pii: S1074-5521(

Inserts

iRFP protein PAS domain
iRFP protein GAF domain (mutated)

Use

Tags

E coil and K coil

Expression

Bacterial

Mutation

F165Y, D232Y, W308R

Promoter

arabinose inducible and rhamnose inducible

Available since

July 26, 2013

Availability

Academic Institutions and Nonprofits only

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pET48 AtGUN1-SMR1

Plasmid

#136361

Purpose

Bacterial expression of recombinant Arabidopsis thaliana GUN1 (amino acids 709 to 918)

Depositor

Tatsuru Masuda

Article

Shimizu et al Proc Natl Acad Sci U S A. 2019 Dec 3;116

Insert

cDNA of Arabidopsis thaliana GUN1 corresponding to amino acids 709 to 918 (GUN1 Mustard Weed)

Use

Tags

TRX-His

Expression

Bacterial

Mutation

Promoter

T7

Available since

Feb. 13, 2020

Availability

Academic Institutions and Nonprofits only

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pET48 AtGUN1-SMR2

Plasmid

#136362

Purpose

Bacterial expression of recombinant Arabidopsis thaliana GUN1 (amino acids 781 to 918)

Depositor

Tatsuru Masuda

Article

Shimizu et al Proc Natl Acad Sci U S A. 2019 Dec 3;116

Insert

cDNA of Arabidopsis thaliana GUN1 corresponding to amino acids 781 to 918 (GUN1 Mustard Weed)

Use

Tags

TRX-His

Expression

Bacterial

Mutation

Promoter

T7

Available since

Feb. 13, 2020

Availability

Academic Institutions and Nonprofits only

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pET48 AtGUN1-PPR1

Plasmid

#136359

Purpose

Bacterial expression of recombinant Arabidopsis thaliana GUN1 (amino acids 232 to 708)

Depositor

Tatsuru Masuda

Article

Shimizu et al Proc Natl Acad Sci U S A. 2019 Dec 3;116

Insert

cDNA of Arabidopsis thaliana GUN1 corresponding to amino acids 232 to 708 (GUN1 Mustard Weed)

Use

Tags

TRX-His

Expression

Bacterial

Mutation

Promoter

T7

Available since

Feb. 13, 2020

Availability

Academic Institutions and Nonprofits only

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pET48 AtGUN1-PPR2

Plasmid

#136360

Purpose

Bacterial expression of recombinant Arabidopsis thaliana GUN1 (amino acids 232 to 619)

Depositor

Tatsuru Masuda

Article

Shimizu et al Proc Natl Acad Sci U S A. 2019 Dec 3;116

Insert

cDNA of Arabidopsis thaliana GUN1 corresponding to amino acids 232 to 619 (GUN1 Mustard Weed)

Use

Tags

TRX-His

Expression

Bacterial

Mutation

Promoter

T7

Available since

Feb. 10, 2020

Availability

Academic Institutions and Nonprofits only

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pBTK1003 (pSL2)

Plasmid

#190998

Purpose

Expresses red chromoprotein on a broad host range RSF1010 origin plasmid

Depositor

Jeffrey Barrick

Article

Elston et al ISME Commun. 2023 May 24;3(1):49. doi: 1

Insert

red chromoprotein

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

CP25

Available since

Oct. 20, 2022

Availability

Academic Institutions and Nonprofits only

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pBbB3k-GFP

Plasmid

#35348

Depositor

Jay Keasling

Article

Lee et al J Biol Eng. 2011 Sep 20;5:12.

Insert

GFP

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

ProS

Available since

Feb. 20, 2012

Availability

Academic Institutions and Nonprofits only

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hisSUMO-CTCF(F1-F9)

Plasmid

#102859

Purpose

Express DNA binding domain of mouse CTCF in E. coli

Depositor

Gary Stormo

Article

Zuo et al Sci Adv. 2017 Nov 17;3(11):eaao1799. doi

Insert

CTCF (Ctcf Mouse)

Use

Tags

hisSUMO

Expression

Bacterial

Mutation

Promoter

T7

Available since

Nov. 22, 2017

Availability

Academic Institutions and Nonprofits only

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pKJ237

Plasmid

#212320

Purpose

Express KnFnuc

Depositor

Jonathan Gootenberg, Omar Abudayyeh

Article

Jiang et al Sci Adv. 2023 Sep 29;9(39):eadk0171. doi

Insert

KnFnuc

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Jan. 2, 2024

Availability

Academic Institutions and Nonprofits only

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pKJ226

Plasmid

#212316

Purpose

Express DpFnuc with pureexpress

Depositor

Jonathan Gootenberg, Omar Abudayyeh

Article

Jiang et al Sci Adv. 2023 Sep 29;9(39):eadk0171. doi

Insert

DpFnuc

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Jan. 2, 2024

Availability

Academic Institutions and Nonprofits only

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pCoofy12

Plasmid

#43977

Depositor

Sabine Suppmann

Article

Scholz et al BMC Biotechnol. 2013 Feb 14;13(1):12.

Type

Empty backbone

Use

Tags

OneStrep

Expression

Bacterial

Mutation

Promoter

T7

Available since

Oct. 28, 2013

Availability

Academic Institutions and Nonprofits only

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pGEX4T3-HA-ERK2 K54R

Plasmid

#53170

Purpose

Expresses GST-HA tagged human ERK2 K54R from bacterial expression vector

Depositor

Christopher Counter

Article

Brady et al Nature. 2014 Apr 9. doi: 10.1038/nature1

Insert

ERK2 (MAPK1 Mustard Weed, Human)

Use

Tags

GST and HA

Expression

Bacterial

Mutation

K54R

Promoter

Available since

June 6, 2014

Availability

Academic Institutions and Nonprofits only
pT7-7 asyn S129A

Plasmid

#36048

Depositor

Hilal Lashuel

Article

Paleologou et al J Biol Chem. 2008 Jun 13;283(24):16895-9

Insert

alpha-synuclein S129A (SNCA Human)

Use

Tags

Expression

Bacterial

Mutation

S129A

Promoter

T7

Available since

July 13, 2012

Availability

Academic Institutions and Nonprofits only

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pE4c

Plasmid

#17464

Purpose

modified pENTR vector for C-terminal GST tag fusion with your gene of interest

Depositor

Giovanna Benvenuto

Article

Dubin et al Plant Methods. 2008 Jan 22. 4(1):3.

Type

Empty backbone

Use

Modified gateway entry vector

Tags

GST

Expression

Bacterial, Insect, Mammalia…

Mutation

Promoter

Available since

March 21, 2008

Availability

Academic Institutions and Nonprofits only

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pQE80L TriCatcher-ELP

Plasmid

#112630

Purpose

Two SpyCatchers linked by an elastin-like polypeptide with a C-terminal SnoopCatcher

Depositor

Mark Howarth

Article

Wieduwild et al Biomaterials. 2018 Oct;180:253-264. doi:

Insert

TriCatcher-ELP

Use

Tags

His6 and TEV cleavage site

Expression

Bacterial

Mutation

Promoter

T5

Available since

Sept. 7, 2018

Availability

Academic Institutions and Nonprofits only

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PANK1

Plasmid

#32871

Purpose

Bacterial expression for structure determination; may not be full ORF

Depositor

Cheryl Arrowsmith

Insert

PANK1 (PANK1 Human)

Use

Tags

His tag (with Thrombin cleavage)

Expression

Bacterial

Mutation

amino acids 231-597 only

Promoter

Available since

Jan. 6, 2012

Availability

Industry, Academic Institutions, and Nonprofits
pGEX-4T1-Claspin-FL-wt

Plasmid

#31621

Depositor

Aziz Sancar

Article

Lindsey-Boltz et al J Biol Chem. 2009 Nov 27. 284(48):33107-

Insert

Claspin (CLSPN Human)

Use

Tags

Flag, GST, and His

Expression

Bacterial

Mutation

Promoter

Available since

Nov. 9, 2011

Availability

Academic Institutions and Nonprofits only
pJS183

Plasmid

#134142

Purpose

Bacterial expression of GFP-Ska1 and Ska2

Depositor

Iain Cheeseman

Article

Monda et al Curr Biol. 2017 Dec 4;27(23):3666-3675.e

Insert

His-GFP-human Ska1; human Ska2

Use

Tags

GFP

Expression

Bacterial

Mutation

Promoter

Available since

Feb. 7, 2020

Availability

Academic Institutions and Nonprofits only

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