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Plasmid#129392PurposeDerived from pAH-CTX1-rha. The native Streptococcus pyogenes dCas9 gene cloned downstream of PrhaBAD. Non-codon-optimized dCas9 version of pAH-CTX1-rhadCas9.DepositorInsertS. pyogenes dCas9
UseCRISPRTagsExpressionBacterialMutationAspartate 10 to Alanine, Histidine 840 to AlaninePromoterPrhaBADAvailable sinceDec. 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
pTriEx-PA-Rac1-C450A
Plasmid#22025PurposeExpression of Rac1 fused with light-insensitive LOV2 (C450A mutation)DepositorInsertPA-Rac1-C450A (RAC1 Avena sativa (oat), Human)
UseTags6X HisExpressionBacterial, Insect, and Mamm…MutationLOV domain contains light-insensitive mutation C4…PromoterCMV, p10Available sinceSept. 29, 2009AvailabilityAcademic Institutions and Nonprofits only -
pGV3319
Plasmid#170277PurposeExpression of mutant Bst polymerase (Bst-LF D720A) for loop mediated isothermal amplification (LAMP)DepositorInsertBst-LF D720A
UseTagsHexahistidineExpressionBacterialMutationD720APromoterT7-lacAvailable sinceJune 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET15b-Casp8 delta DED C360A
Plasmid#11828DepositorInsertCaspase 8 minus Death Effecter Domain catalytic mutant (CASP8 Human)
UseTags6xHisExpressionBacterialMutationMinus Death Defecter Domain (aa 1-216), C360APromoterAvailable sinceMay 12, 2006AvailabilityAcademic Institutions and Nonprofits only -
pGEX-KG-UbcH5b/2443
Plasmid#11477DepositorInsertUbcH5b (UBE2D2 Human)
UseTagsGSTExpressionBacterialMutationn/aPromoterAvailable sinceApril 5, 2006AvailabilityAcademic Institutions and Nonprofits only -
MEK1C222
Plasmid#164638PurposeBacterial expression plasmid for His6-MEK1C222DepositorInsertmitogen-activated protein kinase kinase 1 (MAP2K1 Human)
UseTagsHis6-tagExpressionBacterialMutationG(-19)F/S222C/C277S/C376SPromoterT7Available sinceMay 5, 2021AvailabilityAcademic Institutions and Nonprofits only -
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B1 Integrin-GFP
Plasmid#186138PurposeFor expression of recombinant beta1 integrin cytosolic domain with C-terminal GFP tag in bacteriaDepositorInsertITGB1 (ITGB1 Human)
UseTagsMBP and mEGFPExpressionBacterialMutationPromoterLacAvailable sinceJuly 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDEST17-UbE2U
Plasmid#15810DepositorInsertUbiquitin conjugating enzyme UbE2U (UBE2U Human)
UseTagsHisExpressionBacterialMutationPromoterAvailable sinceSept. 28, 2007AvailabilityAcademic Institutions and Nonprofits only -
pJUMP26-1A(sfGFP)
Plasmid#126973PurposeLevel 1 vector. sfGFP reporter. Origin p15A (medium copy number)DepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionBacterialMutationNonePromoterAvailable sinceJuly 31, 2019AvailabilityAcademic Institutions and Nonprofits only -
pRSET FLIPPi-260n
Plasmid#13552DepositorInsertFLIPPi WT
UseTagsECFP and EYFPExpressionBacterialMutationPromoterAvailable sinceDec. 15, 2006AvailabilityAcademic Institutions and Nonprofits only -
pBbA4a-RFP
Plasmid#35298DepositorInsertRFP
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterProEAvailable sinceFeb. 16, 2012AvailabilityAcademic Institutions and Nonprofits only -
UBH14
Plasmid#28158PurposeBacterial expression for structure determination; may not be full ORFDepositorInsertE3 ligase UHRF2 (UHRF2 Human)
UseTagsHis tag with thrombin cleavageExpressionBacterialMutationSRA Domain (residues 419-648)PromoterAvailable sinceFeb. 28, 2011AvailabilityIndustry, Academic Institutions, and Nonprofits -
pYTK070
Plasmid#65177PurposeEncodes ConR4 as a Type 5 part to be used in the Dueber YTK systemDepositorInsertConR4
UseTagsExpressionBacterialMutationPromoterAvailable sinceJune 16, 2015AvailabilityAcademic Institutions and Nonprofits only -
GC83 pComb3xss anti-Gephyrin nanobody
Plasmid#134721PurposeBacterial expression of anti-Gephyrin (Human) recombinant llama nanobody for use as an immunolabelDepositorInsertanti-Gephyrin (Human) recombinant llama nanobody GC83 (GPHN L. glama (llama))
UseTags6xHis-HAExpressionBacterialMutationPromoterlacAvailable sinceDec. 10, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAL3
Plasmid#114673PurposeT7 vector having very low basal expression of target protein can clone and express almost any gene. Asymmetric ligation clones as many as 3 genes simultaneously for co-expression from the same mRNA.DepositorTypeEmpty backboneUseTagsoptional Ser-Gly-6HisExpressionBacterialMutationPromoterT7Available sinceAug. 15, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET30a-His6-ABP-LOXCATmut
Plasmid#139972PurposeA control for His6-ABP-LOXCAT where LOX is catalytically dead (mutations H265A and R268A)DepositorInsertA fusion of lactate oxidase from A. viridans and catalase from E. coli
UseTagsHis6ExpressionBacterialMutationMutation in H265A and R268A in LOX (used original…PromoterT7Available sinceSept. 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
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pBabe-PI(WT)-Src(KD)-mCherry
Plasmid#87358PurposePI(WT): Photo-inhibitable/blue light sensitive; kinase dead Src mutationDepositorInsertsUseTagsmCherryExpressionBacterial and MammalianMutationD388RPromoterCMVAvailable sinceApril 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
pPhlF-P2
Plasmid#74686PurposePhlF-P2 gateDepositorInsertPTac-RBSP2-PhlF-PPhlF-YFP
UseTagsExpressionBacterialMutationPromoterAvailable sinceApril 29, 2016AvailabilityAcademic Institutions and Nonprofits only