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Plasmid#201182PurposeBst polymerase large fragment (BF) F710Y/D598A, His6-tagged, HRV 3C-cleavableDepositorInsertBst polymerase large fragment (BF) F710Y/D598A
UseTagsHRV 3C protease cleavage site and His6ExpressionBacterialMutationF710Y D598APromoterT5-lacOAvailable sinceSept. 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
pHH0103_PCNA_PCNA
Plasmid#110022PurposeProtein expression and purification of PCNA_PCNADepositorInsertPCNA_PCNA (PCNA Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceOct. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGEX Csk-SH2
Plasmid#46420DepositorInsertCsk (CSK Human)
UseTagsGST and PreScissionExpressionBacterialMutationcontains amino acids 74-177PromoterTacAvailable sinceJuly 12, 2013AvailabilityAcademic Institutions and Nonprofits only -
KBB609
Plasmid#185110PurposePom152 N-terminus in pEX-N-His for bacterial expression and purificationDepositorInsertPOM152
UseTags6HisExpressionBacterialMutationPom152 N-term-6HisPromoterAvailable sinceJuly 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pGEX5X.1 PINK1 KD
Plasmid#13322DepositorInsertPINK1 kinase domain (PINK1 Human)
UseTagsGSTExpressionBacterialMutationAmino acids 112-496; Kinase dead: K219D/D362A/D3…PromoterAvailable sinceNov. 9, 2006AvailabilityAcademic Institutions and Nonprofits only -
pRSET FLIPglu-600uDelta13
Plasmid#13559DepositorInsertFLIPglu F16A Delta13
UseTagsECFP and EYFPExpressionBacterialMutationF16A in binding proteinPromoterAvailable sinceDec. 15, 2006AvailabilityAcademic Institutions and Nonprofits only -
pTH1-SaSrtA-Q
Plasmid#64980PurposeExpresses the E108Q mutant of Staphylococcus aureus sortase A in E. coliDepositorInsertsortase A
UseTagsHis6 tag, Mxe GyrA intein, and chitin-binding pro…ExpressionBacterialMutationdeleted amino acids 1-59, E108QPromoterAvailable sinceJune 19, 2015AvailabilityAcademic Institutions and Nonprofits only -
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pHH0103 NEDD4 WW domain #4
Plasmid#104211PurposeBacterial expression of WW domain #4 from NEDD4DepositorInsertNEDD4 WW domain #4 (NEDD4 Human)
UseTagsGSTExpressionBacterialMutationcodon optimized for expression in bacteriaPromotertacAvailable sinceJan. 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
pVRa03_1198
Plasmid#49655DepositorInsertECF03_1198
UseSynthetic BiologyTagsHis6-PreScissionExpressionBacterialMutationCodon optimized for E.coliPromoterpT7-lacOAvailable sinceJan. 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
pET-CVm-PTEN
Plasmid#168032PurposeExpresses eCFP-PTEN(M265-N329)-mVenus in bacterial cellsDepositorInserteCFP-PTEN(M265-N329)-mVenus
UseTagsHisx6 and mycExpressionBacterialMutationPromoterAvailable sinceApril 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGEX4T3-HA-ERK2 D321N
Plasmid#53202PurposeExpresses GST and HA tagged human ERK2 D321N from bacterial expression vectorDepositorInsertERK2 (MAPK1 Human)
UseTagsGST and HAExpressionBacterialMutationD321NPromoterAvailable sinceJune 24, 2014AvailabilityAcademic Institutions and Nonprofits only -
pBAD-bHs
Plasmid#134259PurposeFor L-arabinose inducible bacterial expression of S. typhimurium codon-optimized hyaluronidase (Streptomyces koganeinsis UniProt A0A0U2E2) with N-terminal 6x His tagDepositorInsertHyaluronidase from Streptomyces koganeinsis codon-optimized for Salmonella typhimurium
UseTags6x HisExpressionBacterialMutationcodon optimized for Salmonella typhimuriumPromoterAraCAvailable sinceNov. 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pGEX4T-1 human RNF146 WWE (99-183)
Plasmid#132613Purposeexpresses human RNF146 WWE (99-183) in E. coliDepositorInsertRNF146 WWE (99-183)
UseTagsExpressionBacterialMutationPromoterAvailable sinceOct. 17, 2019AvailabilityAcademic Institutions and Nonprofits only -
HMBP-3C-STAR.66-285
Plasmid#100094Purposeexpresses MBP-tagged STARD1 protein domain (residues 66-285)DepositorInsertSTAR protein (STAR Human)
UseTagsHis-tagged MBPExpressionBacterialMutationPromoterPtacAvailable sinceSept. 22, 2017AvailabilityAcademic Institutions and Nonprofits only -
pBAD/HisD-TagRFP675
Plasmid#44274DepositorInsertTagRFP675
UseTagsExpressionBacterialMutationM41Q/F80W/S143N/L147M/S158N/D159Y/N173SPromoterpBADAvailable sinceMay 16, 2013AvailabilityAcademic Institutions and Nonprofits only -
pIB170
Plasmid#90193PurposeE. coli - Streptococci shuttle plasmid for gene expression in streptococci with Pveg promoterDepositorTypeEmpty backboneUseTagsNoneExpressionBacterialMutationPromoterAvailable sinceApril 18, 2018AvailabilityAcademic Institutions and Nonprofits only -
pGBHPS-SortA
Plasmid#21930DepositorInsertsortase
UseTagsGB1, HRV3C cleagage site, and HisExpressionBacterialMutationdeleted amino acids 1-59PromoterAvailable sinceOct. 5, 2009AvailabilityAcademic Institutions and Nonprofits only -
pAmtR-A1
Plasmid#74676PurposeAmtR-A1 gateDepositorInsertPTac-RBSA1-AmtR-PAmtR-YFP
UseTagsExpressionBacterialMutationPromoterAvailable sinceMay 9, 2016AvailabilityAcademic Institutions and Nonprofits only -
pLinker-BirA-3xHA-HXGPRT-loxP
Plasmid#86668Purposefor generation of BirA tagged strains using CRISPR tagging technologyDepositorInsertLinker-BirA-3xHA-HXGPRT 3'UTR
UseTagsExpressionBacterialMutationPromoterAvailable sinceMay 4, 2017AvailabilityAcademic Institutions and Nonprofits only