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Plasmid#160881PurposeExpression of N. tabacum Rubisco small subunit (rbcS-S1), Rubisco chaperone protiens rbcX, raf1, bsd2 and A. thaliana Rubisco chaperone proteins raf2 in E. coli.DepositorInsertrbcS-S1, rbcX, raf1, raf2, bsd2
UseTagsExpressionBacterialMutationPromoterrbcS-S1: T7, chaperones: T7Available sinceDec. 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGBW-m4046754
Plasmid#145647PurposeBacterial Expression plasmid for SARS-CoV-2 3C-like proteinaseDepositorInsertSARS-CoV-2 3C-like proteinase (ORF1ab Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
UseTagsCleavable TEV;6xHISExpressionBacterialMutationwtPromoterPromoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…Available sinceApril 29, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pJK615
Plasmid#71699PurposeProduces E. coli thioredoxin with a C-terminal His6 tag (EcTrxAH6)DepositorInsertthioredoxin
UseTagsHis6ExpressionBacterialMutationPromoterT7Available sinceJan. 29, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJK_proB_eGFP
Plasmid#59845PurposeConstitutively expresses eGFP with proB promoterDepositorInsertEnhanced Green Fluorescent Protein
UseTags6x-HisExpressionBacterialMutationS2R,S65G,S72APromoterproBAvailable sinceOct. 20, 2014AvailabilityAcademic Institutions and Nonprofits only -
pET29b-dspB(E184Q)-6xHis
Plasmid#175802PurposePlasmid for overexpression of recombinant His-tagged protein DspB(E184Q), used as a probe for binding to biofilm polysaccharide PNAG.DepositorInsertDispersin B
UseTagsHexahistidine tagExpressionBacterialMutationE184QPromoterT7Available sinceOct. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pRAF-MutL
Plasmid#86607PurposeE. coli MutL expression plasmidDepositorInsertE. coli MutL
UseTagshis-tag and ald-tagExpressionBacterialMutationPromoterT7Available sinceAug. 16, 2017AvailabilityAcademic Institutions and Nonprofits only -
pGEX-4T1-Rac1 G15A
Plasmid#69355Purposeexpression of nucleotide free Rac1 in bacterial cellsDepositorInsertRac1 (RAC1 Human)
UseTagsGSTExpressionBacterialMutationG15APromotertacAvailable sinceSept. 30, 2015AvailabilityAcademic Institutions and Nonprofits only -
pQCascade-array1
Plasmid#140623PurposeExpresses V. cholerae TniQ, Cas8, Cas7, and Cas6 and crRNA-array1. The crRNA-array1 targets 8 different loci in Escherchia coli.DepositorInsertVchTniQ, VchCas8, VchCas7, VchCas6, crRNA-array1
UseCRISPR; TransposonTagsExpressionBacterialMutationPromoterAvailable sinceJuly 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-mMaple3-kanMX6
Plasmid#105165PurposeYeast gene targetingDepositorInsertmMaple3-kanMX6
UseTagsGlySerGly-mMaple3ExpressionBacterial and YeastMutationPromoterAvailable sinceMarch 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
pBS mNFATc3-EE
Plasmid#17868DepositorInsertNFATc3 (Nfatc3 Mouse)
UseTagsEEExpressionBacterialMutationPromoterAvailable sinceApril 29, 2008AvailabilityAcademic Institutions and Nonprofits only -
pEX-A-U6-TelgRNA_PuroR
Plasmid#84782PurposeExpresses telomere-specific sgRNA.DepositorInserttelomere-specific sgRNA
UseCRISPRTagsExpressionBacterial and MammalianMutationPromoterU6Available sinceAug. 9, 2017AvailabilityAcademic Institutions and Nonprofits only -
NEDD4L
Plasmid#25169PurposeBacterial expression for structure determination; may not be full ORFDepositorInsertnedd4l.0574.0947 (NEDD4L Human)
UseTagsHisExpressionBacterialMutationHect DomainPromoterAvailable sinceAug. 9, 2010AvailabilityIndustry, Academic Institutions, and Nonprofits -
pJJDuet30
Plasmid#11962DepositorInsertSsp DnaE-N
UseTagsGB1ExpressionBacterialMutationPromoterAvailable sinceSept. 21, 2007AvailabilityAcademic Institutions and Nonprofits only -
pCAM12
Plasmid#86471PurposeExpresses p12 and p9-mCitrineDepositorInsertp12, p9-mCitrine
UseSynthetic BiologyTagsmCitrineExpressionBacterialMutationPromoterpBADAvailable sinceApril 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pYTK069
Plasmid#65176PurposeEncodes ConR3 as a Type 5 part to be used in the Dueber YTK systemDepositorInsertConR3
UseTagsExpressionBacterialMutationPromoterAvailable sinceJune 16, 2015AvailabilityAcademic Institutions and Nonprofits only -
pSumo-His6-SUMO-AtTPR1(1-209)
Plasmid#177858PurposeBacterial Expression of TPR1DepositorInsertTPR1 (TPR1 Mustard Weed)
UseTags6xHis-SUMOExpressionBacterialMutationPromoterAvailable sinceDec. 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pOGG011
Plasmid#113985PurposeVector construction module. pBBR1 medium-copy number origin of replication for construction of Golden Gate cloning vectorsDepositorInsertpBBR1
UseTagsExpressionBacterialMutationDomesticated for Golden Gate cloningPromoterAvailable sinceJan. 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET-Duet1-6xHis-TEV-LC3BGlydelta5C
Plasmid#190237PurposePlasmid for the expression and purification of 6xHis-TEV-LC3BGlydelta5C. Internal reference: SMC893.DepositorInsertMAP1LC3B (MAP1LC3B Human)
UseTags6xHis-TEVExpressionBacterialMutationPromoterAvailable sinceSept. 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
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pEB1-mEYFP
Plasmid#103985PurposePlasmid encoding mEYFPDepositorInsertmEYFP
UseLow copyTagsExpressionBacterialMutationMutations relative to wild-type GFP (S65G, V68L, …PromoterproCAvailable sinceDec. 13, 2017AvailabilityAcademic Institutions and Nonprofits only