-
Plasmid#180459PurposeE. coli expression of soluble MHC proteinDepositorInsertHLA-A*02:01 (CD8 mut)
UseTagsBSP41ExpressionBacterialMutationD227K, T228A, replacement of transmembrane domain…PromoterAvailable sinceFeb. 24, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAE03
Plasmid#61044PurposeTo construct C-terminal GFP fusions, single crossover integration vectorDepositorTypeEmpty backboneUseSynthetic BiologyTagsGFP+ExpressionBacterialMutationPromoterAvailable sinceFeb. 6, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCS-PluxIlux
Plasmid#47641Purposewt luxI promoter in pCS26DepositorInsertPluxI
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterPluxIAvailable sinceOct. 1, 2013AvailabilityAcademic Institutions and Nonprofits only -
pAJM.718
Plasmid#108519PurposePBetI-YFP Reporter. Cho sensor for use in Marionette strain.DepositorInsertPBetI-YFP
UseTagsExpressionBacterialMutationPromoterAvailable sinceAug. 16, 2018AvailabilityAcademic Institutions and Nonprofits only -
pTXB1-hMeCP2
Plasmid#48091PurposeBacterial expression of Mxe intein/chitin binding domain tagged MeCP2-WTDepositorInsertMethyl-CpG Binding Protein 2 (Rett Syndrome) (MECP2 Human)
UseTagsExpressionBacterialMutationMxe intein/chitin binding domain taggedPromoterAvailable sinceApril 17, 2014AvailabilityAcademic Institutions and Nonprofits only -
TwinStrep-SUMO-NLS-BhCas12b_v4
Plasmid#158615PurposeFor protein expression of BhCas12b_v4DepositorInsertNLS-BhCas12b_v4
UseTags6xHIS-TwinStrep-SUMOExpressionBacterialMutationPromoterAvailable sinceAug. 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
ALIX(P801G)-Strep
Plasmid#180024PurposeCodon-optimized bacterial expression plasmid. Expresses ALIX(1-868, P801G)-Strep. P801G mutation facilitates expression in E. coli.DepositorInsertALIX(1-868, P801G)-Strep (PDCD6IP Human)
UseTagsStrep tag (WSHPQFEK)ExpressionBacterialMutationP801GPromoterAvailable sinceJune 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
gZiPro
Plasmid#198441PurposeActivity/inhibition assays, 19F-NMRDepositorInsertglycine linked Zika virus NS2B-NS3
UseTagsHis6-TEVExpressionBacterialMutationR95*A,PromoterT7 promoterAvailable sinceMarch 29, 2023AvailabilityAcademic Institutions and Nonprofits only -
dCas9-CRY2Clust
Plasmid#200969Purposeinduction of phase-separated condensate by CRY2ClustDepositorInsertCRY2Clust
UseLentiviralTagsExpressionBacterial and MammalianMutationPromoterAvailable sinceMay 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET12a-AL-09 FL
Plasmid#47081DepositorInsertAL-09 (IGKV3-20 Human)
UseTagsompTExpressionBacterialMutationPromoterT7Available sinceAug. 1, 2013AvailabilityAcademic Institutions and Nonprofits only -
pFloat.T7_v1
Plasmid#133070PurposeEntry vector for cloning genes under a T7 promoter for expression in bacteria. Version 1.0.DepositorTypeEmpty backboneUseEntry vectorTags3C-His6 and His6-3CExpressionBacterialMutationPromoterT7Available sinceMay 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
pNIC-PelB-NH10T-AVITAG-GG
Plasmid#215793PurposeCloning of target gene with N terminal PelB SEC pathway signal peptide His10 and AVITAG and TEV cleavageDepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceApril 25, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
pNIC-HIS6-GST-TEV-AVITAG-GG
Plasmid#215809PurposeCloning of target gene with N terminal His6 GST tag and TEV cleavage non cleavable N-terminal avitagDepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceApril 25, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
pRSET-B-frSkyran-S
Plasmid#182209PurposeBacterial expression of frSkylan-SDepositorInsertfrSkylan-S
UseTags6xHisExpressionBacterialMutationPromoterT7Available sinceMay 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
pExp-MsyB
Plasmid#112570PurposeUsed for expression of protein in E. coli as TEV cleavable N-terminual 8His and MsyB (acidic protein MsyB from E. coli) fusions with optional C-terminal StrepII-tagDepositorTypeEmpty backboneUseTags8His and Strep tagExpressionBacterialMutationPromoterAvailable sinceFeb. 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBbS15k-RFP
Plasmid#126194PurposeConstitutive expression vector for Escherichia coli. BglBrick compatible.DepositorInsertRFP
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterJ23100Available sinceJune 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBAD mPlum
Plasmid#91773PurposeBacterial expression of a fluorescent protein, monomeric Plum (mPlum)DepositorInsertmonomeric Plum
UseTagsno tagExpressionBacterialMutationPromoteraraBADAvailable sinceJune 30, 2017AvailabilityAcademic Institutions and Nonprofits only -
pPsrA-R1
Plasmid#74688PurposePsrA-R1 gateDepositorInsertPTac-RBSR1-PsrA-PPsrA-YFP
UseTagsExpressionBacterialMutationPromoterAvailable sinceApril 29, 2016AvailabilityAcademic Institutions and Nonprofits only -
pDSG288
Plasmid#115607Purposesurface-displayed Ag3, used in composability experiments; low copyDepositorInsertregulated adhesin construct
UseTagsExpressionBacterialMutationPromoterAvailable sinceOct. 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
pGEX-4T1-Cdc42 G15A
Plasmid#69356Purposeexpression of nucleotide free Cdc42 in bacterial cellsDepositorInsertCdc42 (CDC42 Human)
UseTagsGSTExpressionBacterialMutationG15APromotertacAvailable sinceSept. 30, 2015AvailabilityAcademic Institutions and Nonprofits only