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Showing: 7721 - 7740 of 28518 results

  2. pET28b-BE3∆UGI

    Plasmid
    #96943
    Purpose
    The plasmid encoding the His6-rAPOBEC1-XTEN-nCas9 protein (BE3∆UGI) was generated by site-directed mutagenesis using pET28b-BE1 (Addgene plasmid #73018).
    Depositor
    Daesik Kim
    Article
    Kim et al Nat Biotechnol. 2017 May;35(5):475-480.
    Insert
    BE3∆UGI
    Use
    Tags
    His6 tag
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    Sept. 20, 2017
    Availability
    Academic Institutions and Nonprofits only
    96943-plasmid-map-sequence-id-193667

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  4. PTPRE

    Plasmid
    #38950
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    PTPRE (PTPRE Human)
    Use
    Tags
    His6
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    May 7, 2013
    Availability
    Academic Institutions and Nonprofits only

  5. J-HARS-4

    Plasmid
    #166529
    Purpose
    scFv of a human scaffold targeting Histidyl-tRNA synthetase. Antigen coverage aa 1-509 of 509, full-length
    Depositor
    Susanne Gräslund
    Article
    Preger et al J Biol Chem. 2020 Oct 9;295(41):13981-13
    Insert
    single chain-Fv, scFv
    Use
    Recombinant antibody expression
    Tags
    3xFLAG, His6 and OmpA leader sequence
    Expression
    Bacterial
    Mutation
    Promoter
    LacZ
    Available since
    April 28, 2021
    Availability
    Academic Institutions and Nonprofits only
    166529-plasmid-map-sequence-id-331465

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  6. L-SARS-7

    Plasmid
    #166527
    Purpose
    scFv of a human scaffold targeting Seryl-tRNA synthetase. Antigen coverage aa 1-514 of 514, full-length
    Depositor
    Susanne Gräslund
    Article
    Preger et al J Biol Chem. 2020 Oct 9;295(41):13981-13
    Insert
    single chain-Fv, scFv
    Use
    Recombinant antibody expression
    Tags
    3xFLAG, His6 and OmpA leader sequence
    Expression
    Bacterial
    Mutation
    Promoter
    LacZ
    Available since
    April 1, 2021
    Availability
    Academic Institutions and Nonprofits only
    166527-plasmid-map-sequence-id-329199

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  7. pMS1: pHelper(AvCAST)_AvPSP1

    Plasmid
    #168133
    Purpose
    Inducible expression of AvCAST proteins with crRNA targeting AvPSP1.
    Depositor
    Feng Zhang
    Article
    Saito et al Cell. 2021 Apr 29;184(9):2441-2453.e18.
    Inserts
    AvCAST minimal CRISPR array (with spacer for AvPSP1)
    AvCAST Cascade proteins (Cas6, Cas8, Cas7 and Cas5)
    AvCAST Tns proteins (TnsA, TnsB, TnsC and TniQ)
    AvTnsD
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    June 2, 2021
    Availability
    Academic Institutions and Nonprofits only
    168133-plasmid-map-sequence-id-330610

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  15. PA-TagRFP-pBad

    Plasmid
    #62793
    Purpose
    Localization: Protein Expression Vector , Excitation:N/A, Emission:N/A
    Depositor
    Michael Davidson
    Type
    Empty backbone
    Use
    Tags
    6xHis and TagRFP
    Expression
    Bacterial
    Mutation
    none
    Promoter
    Available since
    March 12, 2015
    Availability
    Industry, Academic Institutions, and Nonprofits

  18. pHS1477

    Plasmid
    #205970
    Purpose
    MWEE01000013 Cas5-HNH operon (E. coli codon optimized) with His14-tagged Cse2 in pET45b(+)
    Depositor
    Feng Zhang
    Article
    Altae-Tran et al Science. 2023 Nov 24;382(6673):eadi1910.
    Insert
    Cas5-HNH all proteins, with His-tagged Cas11
    Use
    Tags
    14xHis on Cas11
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    Nov. 30, 2023
    Availability
    Academic Institutions and Nonprofits only
    205970-plasmid-map-sequence-id-416229

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  19. pHS1801

    Plasmid
    #205966
    Purpose
    JAGBLH010000220 Cas8-HNH full system (E. coli codon optimized) in pACYCDuet-1 with Lac promoters
    Depositor
    Feng Zhang
    Article
    Altae-Tran et al Science. 2023 Nov 24;382(6673):eadi1910.
    Insert
    Cas8-HNH proteins and CRISPR array
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Lac (proteins), pJ23119 (crRNA)
    Available since
    Nov. 30, 2023
    Availability
    Academic Institutions and Nonprofits only
    205966-plasmid-map-sequence-id-416251

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  20. pHS1232

    Plasmid
    #205969
    Purpose
    MWEE01000013 Cas5-HNH operon (E. coli codon optimized) + CRISPR array in pACYCDuet-1 Lac promoters
    Depositor
    Feng Zhang
    Article
    Altae-Tran et al Science. 2023 Nov 24;382(6673):eadi1910.
    Insert
    Cas5-HNH proteins and CRISPR array
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Lac (proteins), pJ23119 (crRNA)
    Available since
    Nov. 30, 2023
    Availability
    Academic Institutions and Nonprofits only
    205969-plasmid-map-sequence-id-416250

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Showing: 7721 - 7740 of 28518 results