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Plasmid#63897PurposeFor phage display of cDNA librariesDepositorInsertcDNA cloning region
UsePhage displayTagsFosExpressionBacterialMutationPromoterlacZAvailable sinceJuly 21, 2015AvailabilityAcademic Institutions and Nonprofits only -
pQTEV-TAGLN2
Plasmid#31569DepositorInserttransgelin 2 (TAGLN2 Human)
UseTagsHis and TEVExpressionBacterialMutationPromoterAvailable sinceMarch 22, 2013AvailabilityAcademic Institutions and Nonprofits only -
BBa_J72113-BBa_J72103
Plasmid#40785DepositorInsertAraC-pBad + coi + cin + repL + pacA
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceOct. 31, 2012AvailabilityAcademic Institutions and Nonprofits only -
pET3a-Ub-D77
Plasmid#18896DepositorInsertUbiquitin-D77 (UBC Human)
UseTagsExpressionBacterialMutationAspartate residue is added to the C-terminus of t…PromoterAvailable sinceSept. 10, 2008AvailabilityAcademic Institutions and Nonprofits only -
pBTK509
Plasmid#110610PurposeBTK assembled broad-host-range plasmid encoding GFP driven by PA2 promoterDepositorInsertGFP
UseTagsExpressionBacterialMutationPromoterAvailable sinceAug. 21, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMRE-Tn5-141
Plasmid#118525PurposeminiTn5 plasmid to deliver constitutively expressed fluorescent protein genes in bacteriaDepositorInsertmTurquoise2
UseMinitn5 delivery vectorTagsExpressionBacterialMutationPromoterAvailable sinceNov. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
pG6(5'Pro) (P#1477)
Plasmid#14659DepositorInsertUAS repeats
UseTagsExpressionBacterialMutationPromoterAvailable sinceJune 30, 2008AvailabilityAcademic Institutions and Nonprofits only -
pBXNH3CA
Plasmid#47069PurposeFX cloning E.coli expression vector with araBAD promoter, N-terminal 10x His tag, 3C protease cleavage site and C-terminal Avi-tagDepositorTypeEmpty backboneUseTags3C protease site (PreScission site), AVI-tag, and…ExpressionBacterialMutationPromoteraraBADAvailable sinceJan. 15, 2014AvailabilityAcademic Institutions and Nonprofits only -
PET-B-HypaSpCas9-NLS-6xHis
Plasmid#207386PurposeExpression of increased-fidelity (i.e. high-fidelity) SpCas9 variant in bacterial cellsDepositorInsertB-HypaSpCas9
UseCRISPRTags6xHisExpressionBacterialMutationN692A, M694A, Q695A, H698A, amino acids 1005-1013…PromoterT7Available sinceSept. 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
YARS_pNIC-CTB10H
Plasmid#153059PurposeExpression construct for producing Avi tagged Tyrosyl-tRNA synthetaseDepositorInsertYARS (YARS1 Human)
UseTagsTEV-Avi-His10ExpressionBacterialMutationPromoterT7Available sinceJune 29, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBLADE_ONLY_C
Plasmid#168050PurposeExpresses the synthetic gene BLADE FP6 under the constitutive J23101** promoter.DepositorInsertBlue light-inducible AraC dimers in Escherichia coli
UseTagsExpressionBacterialMutationPromoterJ23101**Available sinceMay 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
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MT2a
Plasmid#105693PurposeBacterial expression plasmids for production of recombinant metallothionein 2aDepositorInsertMetallothionein 2a (MT2A Human)
UseTagsChiting Binding Domanin-InteinExpressionBacterialMutationPromoterAvailable sinceApril 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
ETV5
Plasmid#73234PurposeBacterial expression for structure determination; may not be full ORFDepositorInsertETV5 (ETV5 Human)
UseTagsHis-6-TEVExpressionBacterialMutationpfam00178:Ets 368-450PromoterT7Available sinceApril 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
ETV4A-c072
Plasmid#53638Purposebacterial expression plasmidDepositorInsertETV4A
UseTagsHis-6-TEVExpressionBacterialMutationBacterial expression for structure determination;…PromoterT7Available sinceJune 3, 2014AvailabilityAcademic Institutions and Nonprofits only -
pNIC-ZB-SPRTNwt-FL
Plasmid#110217PurposeExpression in E. coli of full-length wild-type SPRTN protein, with His and ZB tags at N-terminus.DepositorInsertSPRTN (SPRTN Human)
UseTagsExpressionBacterialMutationP296L (see depositor comments below)PromoterAvailable sinceMay 23, 2018AvailabilityAcademic Institutions and Nonprofits only -
pBS chick islet1 [TJ#44]
Plasmid#16273DepositorInsertislet1 (ISL1 Chicken)
UseTagsExpressionBacterialMutationPromoterAvailable sinceFeb. 4, 2009AvailabilityAcademic Institutions and Nonprofits only -
pEW4: pET-32a Dot1L(2-386)
Plasmid#124099PurposeDot1L(2-386) expression plasmidDepositorInsertDot1L(2-386) (DOT1L Human)
UseTagsTrx-His-TEVExpressionBacterialMutationPromoterT7Available sinceApril 2, 2019AvailabilityAcademic Institutions and Nonprofits only -
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pZH517
Plasmid#102670PurposeTetracycline inducible expression of GFPmut2 using autoregulation; strong ribosome binding site; GFPmut2 ORF can be replaced with gene of interest by isothermal assemblyDepositorInsertGFPmut2
UseTagsExpressionBacterialMutationPromoterAvailable sinceDec. 5, 2017AvailabilityAcademic Institutions and Nonprofits only