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Plasmid#122631PurposeBglFusion compatible integration vector for Streptococcus pneumoniaeDepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceAug. 16, 2019AvailabilityAcademic Institutions and Nonprofits only
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pTH1-SpSrtA
Plasmid#64987PurposeExpresses the wild type of Streptococcus pyogenes sortase A in E. coliDepositorInsertS. pyogenes sortase A
UseTagsHis6 tag, Mxe GyrA intein, and chitin-binding pro…ExpressionBacterialMutationdeleted amino acids 1-81PromoterT7 promoterAvailable sinceJune 5, 2015AvailabilityAcademic Institutions and Nonprofits only -
pYH71
Plasmid#217381PurposepTrc99A-mCherry-mcdB for bacterial expressionDepositorInsertmcdB
UseTagsmCherryExpressionBacterialMutationPromoterAvailable sinceApril 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28(+)-NudC
Plasmid#175022PurposePurification of NudC enzymeDepositorInsertnudC
UseTags6xHisExpressionBacterialMutationPromoterT7Available sinceAug. 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pQTEV-PRKRA
Plasmid#31571DepositorInsertprotein kinase, interferon-inducible double stranded RNA dependent activator (PRKRA Human)
UseTagsHis and TEVExpressionBacterialMutationPromoterAvailable sinceMarch 22, 2013AvailabilityAcademic Institutions and Nonprofits only -
pET29b-EPN01*-His
Plasmid#84509PurposeE.coli version of EPN01*DepositorInsertEPN01*-His_pET29b
UseTagsHis tagExpressionBacterialMutationPromoterAvailable sinceFeb. 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
pSL0374 (His-MBP-Cas8_Cas7_Cas6)
Plasmid#130639PurposeExpresses V. cholerae CAST Cas8, Cas7, and Cas6 from a T7 promoter, with N-terminal His10-MBP-TEVsite tag on Cas8 for purification.DepositorInsertVchCas8, VchCas7, VchCas6
UseCRISPR; TransposonTagsHis10-MBP-TEVsite on Cas8ExpressionBacterialMutationPromoterT7Available sinceSept. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBbS6c-RFP
Plasmid#35293DepositorInsertRFP
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterLlacO-1Available sinceFeb. 16, 2012AvailabilityAcademic Institutions and Nonprofits only -
SIRT5H158Y-pET15b
Plasmid#81032PurposeBacterial expression of Human catalytic mutant SIRT5 proteinDepositorInsertSirt5 (SIRT5 Human)
UseTagsHisExpressionBacterialMutationH158Y catalytic mutantPromoterT7Available sinceAug. 15, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCYC2
Plasmid#43970DepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionBacterial and YeastMutationPromoterCYC1Available sinceApril 9, 2013AvailabilityIndustry, Academic Institutions, and Nonprofits -
pJMP1354
Plasmid#119275Purposefor cloning new sgRNAsDepositorInsertdcas9
UseTagsExpressionBacterialMutationD10A and H840APromoterAvailable sinceJan. 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
KTK_354
Plasmid#180525PurposeKTK compatible Intergration/suicide plasmid. Contains a LacZ dropout cassette flanked by regions homolgous to the arsenic resistance cassete.DepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceApril 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDSG290
Plasmid#115609Purposesurface-displayed Nb3, used in composability experiments; low copyDepositorInsertregulated adhesin construct
UseTagsExpressionBacterialMutationPromoterAvailable sinceOct. 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
pJExpress414_GmPOPB
Plasmid#92234PurposeExpress wild type prolyl oligopeptidase from Galerina marginataDepositorInsertProlyl oligopeptidase B from Galerina marginata
UseTags6x-Histag, TEV cleavage siteExpressionBacterialMutationnonePromoterT7 promoterAvailable sinceAug. 28, 2017AvailabilityAcademic Institutions and Nonprofits only -
pKSJ155
Plasmid#27126DepositorInsertColicin Ia + Ia immunity protein
UseTagsHis6ExpressionBacterialMutationPromoterAvailable sinceJan. 20, 2011AvailabilityAcademic Institutions and Nonprofits only -
pGEX-PAK2 wt
Plasmid#31671DepositorInsertp21-activated protein kinase 2 (PAK2 Human)
UseTagsGSTExpressionBacterialMutationPromoterAvailable sinceApril 11, 2012AvailabilityAcademic Institutions and Nonprofits only -
pBAD-FOLD6
Plasmid#186547PurposeFOLD6 "hyperfolder" green fluorescent protein: bacterial expression plasmidDepositorInsertFOLD6
UseTags6xHisExpressionBacterialMutationmGreenLantern-C48S/V68L/C70V/K101E/T105Y/S147P/K1…PromoteraraBAvailable sinceNov. 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSCARS1
Plasmid#174868PurposeContains TEF1p-TEF1in-hGFP-CYCt cassette and wildtype ARS1DepositorInsertARS1
UseSynthetic BiologyTagsExpressionBacterial and YeastMutationPromoterAvailable sinceOct. 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
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pGEX4T1-GST-LC3B
Plasmid#216836PurposeFor expression in E. coli of GST-LC3B (Gly exposed) (Internal ref. SMC1463)DepositorInsertLC3B (Gly exposed) (MAP1LC3B Human)
UseTagsGSTExpressionBacterialMutationPromoterTAC promoterAvailable sinceApril 19, 2024AvailabilityAcademic Institutions and Nonprofits only