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pBSV2G_PflaB-msfGFPBb

Plasmid

#118231

Purpose

Gentamicin-resistant E.coli/B. burgdorferi shuttle vector; Encodes flagellin promoter driven msfGFP

Depositor

Christine Jacobs-Wagner

Article

Takacs et al Appl Environ Microbiol. 2018 Oct 12. pii

Insert

flagellin promoter driven msfGFP

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Nov. 20, 2018

Availability

Academic Institutions and Nonprofits only

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pMG059 MBP-CREB-His

Plasmid

#154076

Purpose

Expresses MBP-CREB-His (human CREB as a fusion protein with MBP and His- tags) in E.coli

Depositor

Jesse Zalatan

Article

Gavagan et al Cell Syst. 2020 Jun 24;10(6):515-525.e5.

Insert

CREB1 (CREB1 Human)

Use

Tags

His6 and MBP

Expression

Bacterial

Mutation

Promoter

Tac

Available since

Jan. 25, 2021

Availability

Academic Institutions and Nonprofits only

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pET28a-pro-sumo2

Plasmid

#25102

Depositor

Guy Salvesen

Article

Mikolajczyk et al J Biol Chem. 2007 Sep 7. 282(36):26217-2

Insert

pro-SUMO2 (SUMO2 Human)

Use

Tags

His

Expression

Bacterial

Mutation

Promoter

Available since

June 24, 2010

Availability

Academic Institutions and Nonprofits only
pET28-H12TEV-hEIF5B-1~1220(FL)

Plasmid

#208636

Purpose

for E. coli expression of human eIF5B (ENST00000289371)

Depositor

Quanfu Ma

Insert

human eIF5B (ENST00000289371) (EIF5B Human)

Use

Tags

6xhis

Expression

Bacterial

Mutation

Contain SNP rs1376443, protein sequence not chang…

Promoter

T7

Available since

May 20, 2024

Availability

Academic Institutions and Nonprofits only

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CAGGS-EGFP-NLS-3XFLAG-V5

Plasmid

#196090

Purpose

Expresses gfp in mammalian cells. Contains a c-terminal 3xFlag tag and a c-terminal V5 tag

Depositor

Mauro Calabrese

Article

Cherney et al RNA. 2023 Jul 19:rna.079569.122. doi: 10

Insert

GFP

Use

Tags

C-terminal 3xFLAG and C-terminal V5

Expression

Bacterial and Mammalian

Mutation

Promoter

CAGGs

Available since

Sept. 5, 2023

Availability

Academic Institutions and Nonprofits only

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pCLIP18

Plasmid

#125142

Purpose

General purpose cloning vector

Depositor

Chris Boyd, David Sherratt

Article

Boyd et al Gene. 1995 Feb 3;153(1):57-62.

Type

Empty backbone

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

June 7, 2019

Availability

Academic Institutions and Nonprofits only

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human p53-(1-320)

Plasmid

#24864

Depositor

Cheryl Arrowsmith

Article

Ayed et al Nat Struct Biol. 2001 Sep . 8(9):756-60.

Insert

p53 (TP53 Human)

Use

Tags

His

Expression

Bacterial

Mutation

Contains aa 1-320

Promoter

Available since

Aug. 23, 2010

Availability

Academic Institutions and Nonprofits only
pGEX-4T Syt1 C2AB(140) WT

Plasmid

#134695

Purpose

Encodes cytoplasmic domain of synaptotagmin-1 for bacterial expression and purification

Depositor

Edwin Chapman

Article

Bradberry et al J Biol Chem. 2019 Jul 12;294(28):10942-1

Insert

Synaptotagmin 1 (Syt1 Rat)

Use

Tags

GST

Expression

Bacterial

Mutation

Promoter

Available since

Nov. 8, 2021

Availability

Academic Institutions and Nonprofits only

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pp2.1-PT5-lacI-gusA-specR-pp2.2-IMBB

Plasmid

#30505

Depositor

Ichiro Matsumura

Article

Murin et al Appl Environ Microbiol. 2011 Oct 21.

Insert

beta-glucuronidase (gusA Escherichia coli)

Use

Tags

His

Expression

Bacterial

Mutation

Promoter

Available since

Nov. 17, 2011

Availability

Academic Institutions and Nonprofits only

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pSEVA221MBPc-AID-T7RNAP

Plasmid

#167975

Purpose

Expresses fusion protein MBPc-AID-T7RNAP in E. coli

Depositor

Luis Ángel Fernández

Article

Alvarez et al Nat Commun. 2020 Dec 22;11(1):6436. doi:

Insert

MBPc-AID-T7RNAP

Use

Tags

MBPc and T7RNAP

Expression

Bacterial

Mutation

Promoter

TetR-Ptet

Available since

June 17, 2021

Availability

Academic Institutions and Nonprofits only

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pCold TDP43 M337V

Plasmid

#27453

Depositor

Aaron Gitler

Article

Johnson et al J Biol Chem. 2009 Jul 24. 284(30):20329-

Insert

TDP-43 (TARDBP Human)

Use

Tags

Factor Xa and His6

Expression

Bacterial

Mutation

Methionine 337 mutated to Valine (M337V)

Promoter

Available since

Feb. 10, 2011

Availability

Academic Institutions and Nonprofits only
Reb_79 aka PAS620

Plasmid

#71225

Purpose

Reb_1 with RebA(Q91R)

Depositor

Pamela Silver

Article

Polka et al ACS Synth Biol. 2016 Feb 3.

Insert

Reb locus

Use

Tags

none

Expression

Bacterial

Mutation

RebA( G53A, Q91R)

Promoter

Available since

April 7, 2016

Availability

Academic Institutions and Nonprofits only
pET-Duet-1-NONO(53-312)

Plasmid

#135442

Purpose

Expresses NONO 53-312 in the second site; for co-expression with His-tagged protein

Depositor

Charles Bond

Article

Lee et al Nucleic Acids Res. 2015 Apr 20;43(7):382

Insert

Non-POU domain-containing octamer-binding protein (NONO Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7

Available since

Feb. 11, 2020

Availability

Academic Institutions and Nonprofits only

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pRSET-WHaloCaMP1b-EGFP

Plasmid

#205305

Purpose

Expression of WHaloCaMP1b-EGFP fusion in E. coli

Depositor

Eric Schreiter

Article

Farrants et al bioRxiv. 2023 Jul 19:2023.07.18.549527.

Insert

WHaloCaMP1b-EGFP

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Aug. 18, 2023

Availability

Academic Institutions and Nonprofits only

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pJMP1358

Plasmid

#119277

Purpose

for cloning new sgRNAs

Depositor

Carol Gross, Jason Peters, Oren Rosenberg

Article

Peters et al Nat Microbiol. 2019 Jan 7. pii: 10.1038/

Insert

dcas9

Use

Tags

Expression

Bacterial

Mutation

D10A and H840A

Promoter

Available since

Jan. 14, 2019

Availability

Academic Institutions and Nonprofits only

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pOPINK-OTUD5 (OTU, aa 171-358)

Plasmid

#61415

Purpose

Expresses human OTUD5 (OTU domain) in E. coli.

Depositor

David Komander

Article

Mevissen et al Cell. 2013 Jul 3;154(1):169-84. doi: 10.

Insert

OTUD5 (OTUD5 Human)

Use

Tags

His6-GST-3C

Expression

Bacterial

Mutation

Deleted aa 1-170 and aa 359-571.

Promoter

T7

Available since

March 12, 2015

Availability

Academic Institutions and Nonprofits only

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p_SNIPR_target_mut_36

Plasmid

#139466

Purpose

T7 RNAP-driven expression of a non-cognate SNIPR trigger RNA with a mutation at position 36

Depositor

Alexander Green

Article

Hong et al Cell. 2020 Feb 25. pii: S0092-8674(20)30

Insert

Target_mut36

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 23, 2020

Availability

Academic Institutions and Nonprofits only

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BAT8 (3RJW)

Plasmid

#28125

Purpose

Bacterial expression for structure determination; may not be full ORF

Depositor

Cheryl Arrowsmith

Insert

BAT8 (EHMT2 Human)

Use

Tags

His-tag with integrated thrombin site

Expression

Bacterial

Mutation

contains amino acid residues 913-1193.

Promoter

Available since

Feb. 10, 2012

Availability

Industry, Academic Institutions, and Nonprofits
As2

Plasmid

#123160

Purpose

Arsenic sensor used in the sensor cell array. pSB4A3 carrying J109-30arsR-t-ParsR-ABS84-30hrpR-30hrpS-t-PhrpLE-30gfp-t

Depositor

Baojun Wang

Article

Wan et al Nat Chem Biol. 2019 May;15(5):540-548. d

Insert

J109-30arsR-t-ParsR-ABS84-30hrpR-30hrpS-t-PhrpLE-30gfp-t

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

J109, ParsR-ABS84, PhrpLE

Available since

May 9, 2019

Availability

Academic Institutions and Nonprofits only

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pJK217

Plasmid

#72471

Purpose

Produces Acetobacter aceti 1023 citrate synthase with N-terminal His6 tag (H6AaAarA)

Depositor

T. J. Kappock

Insert

citrate synthase

Use

Tags

His6

Expression

Bacterial

Mutation

Promoter

T7

Available since

Feb. 23, 2016

Availability

Academic Institutions and Nonprofits only

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