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Plasmid#71226PurposeReb_1 with RebB(I67T, N84I)DepositorInsertReb locus
UseTagsnoneExpressionBacterialMutationRebB(I67T, N84I)PromoterAvailable sinceApril 7, 2016AvailabilityAcademic Institutions and Nonprofits only -
pGEX-4T HHARI RBR
Plasmid#31254DepositorInsertHuman Homologue of Ariadne (HHARI) (ARIH1 Human)
UseTagsGSTExpressionBacterialMutationRBR domain residues 177-395PromoterAvailable sinceAug. 17, 2011AvailabilityAcademic Institutions and Nonprofits only -
pSKA413
Plasmid#80381PurposeConstitutive CcaS and CcaR with gfpmut3 under the cpcG2Δ59 promoter.DepositorInsertsccaS (complement)
ccaR (complement)
gfpmut3
UseSynthetic Biology; OptogeneticsTagsFLAG tagExpressionBacterialMutationPromoterccaR and cpcG2Δ59Available sinceOct. 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
pTE104
Plasmid#59399PurposeMethyloBrick expression vector, coxB promoter; TcRDepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionBacterialMutationPromoterPcoxBAvailable sinceDec. 30, 2014AvailabilityAcademic Institutions and Nonprofits only -
6xHis(TEV)-dDrp1-StrepII
Plasmid#174421PurposeExpresses D. melanogaster Drp1 in bacteriaDepositorInsertDrp1 (Drp1 Fly)
UseTags6xHis(TEV) and StrepIIExpressionBacterialMutationNonePromoterT7Available sinceSept. 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
pKP968
Plasmid#167653PurposeExpress colicinB-His6, D17C, M261CDepositorInsertcba-his6 D17C, M261C
UseTagsExpressionBacterialMutationcba-his6 with D17C, M261C mutationsPromoterAvailable sinceJuly 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGBW-m4136458
Plasmid#149531PurposeBacterial expression plasmid for SARS-CoV-2 surface glycoprotein (Spike protein)DepositorInsertSARS-CoV-2 S (surface glycoprotein) (S Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
UseTags6xHIS;Linker_GGSG;SUMO tag;Linker_GGSG;Cleavable …ExpressionBacterialMutationwtPromoterPromoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…Available sinceMay 13, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pKM596
Plasmid#8837DepositorTypeEmpty backboneUseTagsE. coli MBPExpressionBacterialMutationPromoterAvailable sinceFeb. 14, 2006AvailabilityAcademic Institutions and Nonprofits only -
pMBP-LbCas12a D832A
Plasmid#113517PurposeExpresses His-MBP fused to LbCas12a with D832A mutation to inactivate RuvC nucleaseDepositorInsertLbCas12a
UseTagsMBPExpressionBacterialMutationD832APromoterT7Available sinceJuly 31, 2018AvailabilityAcademic Institutions and Nonprofits only -
pGal CycK (P#844)
Plasmid#14664DepositorInsertCycK (CCNK Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceJune 30, 2008AvailabilityAcademic Institutions and Nonprofits only -
pB2H2*
Plasmid#163834PurposeTwo-hybrid system that confers spectinomycin resistance in response to substrate phosphorylation. Constitutive expression of c-src kinase, CDC37, and TCPTP(R222M)DepositorInsertSpecR
UseSynthetic BiologyTagsExpressionBacterialMutationTCPTP R222MPromoterPro1;ProD;pLacZ-OptAvailable sinceJuly 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET21-pelB-LaG-11
Plasmid#172762PurposeBacterial expression of HIS-tagged anti-GFP nanobody LaG-11; HIS tag preceded by HRV 3C/PreScission cleavage site.DepositorInsertLaG-11
UseTags6xHIS and pelB leader for periplasmic secretionExpressionBacterialMutationPromoterT7Available sinceAug. 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pETM6-RgTALsyn-HpaBC
Plasmid#100949PurposeExpression of RgTALsyn, HpaB, and HpaC from inducible T7 promoterDepositorInsertsRgTALsyn
HpaB
HpaC
UseSynthetic BiologyTagsExpressionBacterialMutationSilent mutation to remove internal NdeI site on T…PromoterT7Available sinceSept. 15, 2017AvailabilityAcademic Institutions and Nonprofits only -
pDEST17-hOPTN
Plasmid#23053DepositorInsertoptineurin (OPTN Human)
UseTagsHisExpressionBacterialMutationN/APromoterAvailable sinceMay 3, 2010AvailabilityAcademic Institutions and Nonprofits only -
pEM09
Plasmid#135479PurposeSpizellomyces Hsp70 promoter driving expression of Hygromycin resistance fused to GFPDepositorInsertSpunHsp70pr
UseTagsGFP and hphExpressionBacterial and YeastMutationPromoterSpun Hsp70prAvailable sinceMay 19, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA6.MCV.cLT206.V5(CM2B4)
Plasmid#28189DepositorInsertMCPyV Large T antigen (206 wild-type strain)
UseTagsV5ExpressionBacterial and MammalianMutationPromoterAvailable sinceSept. 28, 2011AvailabilityAcademic Institutions and Nonprofits only -
pBSMrnaStrp
Plasmid#67508PurposeExpress the tRNAMet scaffold with the streptavidin aptamer tagDepositorInsertmodified tRNAMet with streptavidin aptamer
UseTagsExpressionBacterialMutationPromoterlppAvailable sinceSept. 15, 2015AvailabilityAcademic Institutions and Nonprofits only -
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pBbA8c-RFP
Plasmid#35272DepositorInsertRFP
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterBADAvailable sinceFeb. 16, 2012AvailabilityAcademic Institutions and Nonprofits only -
pB1H1-Zif268
Plasmid#12612DepositorInsertZif268 in pB1H1 (Egr1 Mouse)
UseTagsExpressionBacterialMutationPromoterAvailable sinceOct. 3, 2006AvailabilityAcademic Institutions and Nonprofits only