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Plasmid#149494PurposeBroad-Host range expression vector, RK2 and pUC origin, CymR(AM) repressor, PCymRC promoter, mRFP, Hygromycin Resistance Marker.DepositorInsertCymR(AM)-mRFP
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterPCymRCAvailable sinceMarch 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAJM.721
Plasmid#108522PurposePSalTTC-YFP Reporter. Sal sensor for use in Marionette strain.DepositorInsertPSalTTC-YFP
UseTagsExpressionBacterialMutationPromoterAvailable sinceAug. 16, 2018AvailabilityAcademic Institutions and Nonprofits only -
pUC18-mini-Tn7T
Plasmid#64954Purposemini-Tn7 base vector with transcriptional terminatorDepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceJuly 6, 2015AvailabilityIndustry, Academic Institutions, and Nonprofits -
CoV-2-NSP3-c001
Plasmid#173083PurposeExpression of recombinant fusion protein in E. coliDepositorInsertrep (ORF1ab Severe acute respiratory syndrome coronavirus 2 (2019-nCoV) (SARS-CoV-2))
UseTagsmCeruleanExpressionBacterialMutationE1084-E1192, codon optimizedPromoterAvailable sinceDec. 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET19b+H2B(T116C)
Plasmid#157808PurposeExpression of human histone H2B with T116C mutation to facilitate maleimide chemistryDepositorInsertH2B(T116C)
UseTagsExpressionBacterialMutationT116CPromoterT7Available sinceAug. 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET23a_mod_HuD_FL
Plasmid#65751Purposeplasmid for bacterial overexpression of HuDDepositorInsertELAVL4 (ELAVL4 Human)
UseTags6X His and FLAG HAExpressionBacterialMutationPromoterT7Available sinceJune 4, 2015AvailabilityAcademic Institutions and Nonprofits only -
TSTA3A-c013
Plasmid#53663Purposebacterial expression plasmidDepositorInsertTSTA3A
UseTagsHis6-TEVExpressionBacterialMutationBacterial expression for structure determination;…PromoterAvailable sinceJune 3, 2014AvailabilityAcademic Institutions and Nonprofits only -
EcRho
Plasmid#113121PurposeExpresses E. coli RhoDepositorInsertRho
UseTagsMGHExpressionBacterialMutationPromoterT7Available sinceOct. 14, 2020AvailabilityAcademic Institutions and Nonprofits only -
pOPINTRX
Plasmid#41116DepositorTypeEmpty backboneUseTagsExpressionBacterial, Insect, and Mamm…MutationPromoterAvailable sinceMarch 27, 2013AvailabilityAcademic Institutions and Nonprofits only -
Reb_221 aka PAS628
Plasmid#71233PurposeReb_1 with RebA(G90A)DepositorInsertReb locus
UseTagsnoneExpressionBacterialMutationRebA(G53A, G90A)PromoterAvailable sinceApril 7, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAWP78-PVCpnf_pvc13-antiHistoneNb
Plasmid#198293PurposeProduces PVCs retargeted with A9 anti-histone nanobodyDepositorInsertPVCpnf_pvc13-antiHistoneNb
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 29, 2023AvailabilityAcademic Institutions and Nonprofits only -
pSEVA231-CRISPR
Plasmid#138712PurposePlasmid designed to clone a specific spacer into BsaI sites to generate a CRISPR ArrayDepositorTypeEmpty backboneUseCRISPR and Synthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceSept. 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCJR01
Plasmid#187888PurposeEscherichia coli plasmid with cloned regions of homology to Campylobacter jejuni chromosome flanking a NotI restriction site. pCJR01 functions as a suicide vector for integrating cloned NotI DNA.DepositorInsertDNA insert has homology to a rRNA region of the Campylobacter jejuni chromosome with an added central NotI restriction site
UseTagsExpressionBacterialMutationPromoternoneAvailable sinceAug. 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
csr-1_son-1_iRFP670
Plasmid#191749PurposeTest fluorescent properties in Neurospora crassa. Nuclear envelope fluorescent reporter.DepositorInsertNCU04288 (NCU04288 Neurospora crassa)
UseTagsNCU04502 promoter and iRFP670ExpressionBacterialMutationPromoterAvailable sinceDec. 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMCSG53_Nsp15
Plasmid#167265PurposeBacterial expression of Nsp15DepositorInsertNsp15 (residues 1-346) (ORF1ab SARS-CoV-2)
UseTagsHis6-TEV siteExpressionBacterialMutationnonePromoterAvailable sinceMay 5, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
pET Trx His6 LIC cloning vector (2Tc-T)
Plasmid#37239DepositorTypeEmpty backboneUseTagsHis6 and Thioredoxin (Trx)ExpressionBacterialMutationPromoterT7Available sinceAug. 7, 2012AvailabilityAcademic Institutions and Nonprofits only -
5KKP (PUS7)
Plasmid#101878PurposeBacterial expression for structure determination; may not be full ORFDepositorInsertPUS7 (PUS7 Human)
UseTags6xHis, TEVExpressionBacterialMutationPromoterT7Available sinceApril 30, 2018AvailabilityIndustry, Academic Institutions, and Nonprofits -
pETM33_CALM1_EF-hand
Plasmid#178468PurposeBacterial expression of human domain with His-tag and GST tagDepositorInsertCALM1_EF-hand (CALM1 Synthetic, Human)
UseTagsHis-GSTExpressionBacterialMutationPromoterT7/LacOAvailable sinceMarch 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTXB3-Flag PLK1
Plasmid#68271Purposebacterial expression of Plk1DepositorInsertPLK1 (PLK1 Human)
UseTagsFlag and Mxe intein/chitin binding domainExpressionBacterialMutationPromoterT7Available sinceAug. 11, 2015AvailabilityAcademic Institutions and Nonprofits only -
pET-51b(+) ChemoG5
Plasmid#193805PurposeProtein production of ChemoG5 in bacteriaDepositorInsertChemoG5
UseTags10xHisExpressionBacterialMutationEGFP:A206K, T225R; HT7: E143R, E147R, L271EPromoterT7Available sinceJune 9, 2023AvailabilityAcademic Institutions and Nonprofits only