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Plasmid#22837DepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceJan. 28, 2010AvailabilityAcademic Institutions and Nonprofits only
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pUS252b
Plasmid#191831PurposeExpresses fuGFPb constitutively in E.coli cells. Designed to act as modular template for cutting out or amplifying fuGFPb to place in other plasmid backbonesDepositorInsertfuGFPb
UseTagsExpressionBacterialMutationPromoterAvailable sinceJan. 17, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits -
pCC1-4k-BBclone2
Plasmid#137070PurposeE. coli plasmid that contains the expression optimized genes for BB0323, P13, DipA and Lmp1DepositorInsertsBB0323
P13
DipA
Lmp1
UseTagsExpressionBacterialMutationwild-type, full-length (signal-peptide-containing…PromoterAvailable sinceMay 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
pH
Plasmid#64178Purposeharbours marker gene HIS3DepositorTypeEmpty backboneUseTagsExpressionBacterial and YeastMutationPromoterAvailable sinceSept. 20, 2016AvailabilityAcademic Institutions and Nonprofits only -
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FGFR1_HUMAN_D0
Plasmid#79719PurposeThis plasmid encodes the kinase domain of FGFR1. Intended for co-expression with YopH Phosphatase that accompanies this set to enhance bacterial kinase expression.DepositorInsertFGFR1 (FGFR1 Human)
UseTagsHis10-TEVExpressionBacterialMutationPromoterT7Available sinceNov. 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
pReporter_5
Plasmid#60566PurposeA reporter plasmid containing the attB and attP sites flanking a green fluorescent protein reporter gene (gfp).DepositorInsertReporter_5
UseTagsExpressionBacterialMutationPromoterBBa_J23119Available sinceMarch 16, 2015AvailabilityAcademic Institutions and Nonprofits only -
pEB1-mClover3
Plasmid#103994PurposePlasmid encoding mClover3DepositorInsertmClover3
UseLow copyTagsExpressionBacterialMutationMutations relative to wild-type GFP (S30R, Y39N, …PromoterproCAvailable sinceDec. 14, 2017AvailabilityAcademic Institutions and Nonprofits only -
pET15b I-SceI (Ctermhis -MH)
Plasmid#195613PurposeExpresses the wild-type I-SceI homing endonuclease from the T7 promoterDepositorInsertI-SceI
UseTagsExpressionBacterialMutationNonePromoterT7Available sinceFeb. 1, 2023AvailabilityAcademic Institutions and Nonprofits only -
pDW363
Plasmid#8842DepositorTypeEmpty backboneUseTagsMBP and biotin acceptor peptideExpressionBacterialMutationPromoterAvailable sinceApril 19, 2006AvailabilityAcademic Institutions and Nonprofits only -
pBXC3GH
Plasmid#47070PurposeFX cloning E.coli expression vector with araBAD promoter and C-terminal 3C protease cleavage site, GFP and 10x His tagsDepositorTypeEmpty backboneUseTags3C protease site (PreScission site), GFP, and dec…ExpressionBacterialMutationPromoteraraBADAvailable sinceJan. 15, 2014AvailabilityAcademic Institutions and Nonprofits only -
pBLxR5
Plasmid#167504PurposeBroad-Host range expression vector, pBBR origin, LuxR activator, PLuxB promoter, mRFP, Gentamicin Resistance Marker.DepositorInsertLuxR-mRFP
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterPLuxBAvailable sinceOct. 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGGC_RT_mScarlet
Plasmid#136967PurposeGoldengate/Greengate CDS module, containing mScarletDepositorInsertmScarlet
UseTagsExpressionBacterialMutationPromoterAvailable sinceJan. 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
MSP2262
Plasmid#70703PurposeBacterial expression plasmid for Sa-dCas9 (D10A/H557A) & sgRNA targeted to site 1: T7-humanSadCas9-NLS-3xFLAG-T7-Sa-sgRNA(84) #1DepositorInsertsmammalian codon-optimized Staphylococcus aureus dCas9
SaCas9 sgRNA (+84)
UseCRISPRTagsNLS-3xFLAGExpressionBacterialMutationD10A & H557A in SaCas9PromoterT7Available sinceDec. 4, 2015AvailabilityAcademic Institutions and Nonprofits only -
pET51b(+)_SNAP-p24
Plasmid#130718PurposePlasmid encoding the bacterial expression of a SNAP-fusion of p24 HIV Capsid proteinDepositorInsertSNAP-Tag-p24
UseTagsHisx10 and StrepTagExpressionBacterialMutationDeleted first Met, Ala and Last Ser from the Enha…PromoterAvailable sinceAug. 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCJH021_SC101_pLAC_Cas12c2_Kan
Plasmid#183072PurposeWild-type Cas12c2 (Yan et al., 2019) in a plasmid containing a SC101 origin and lac promoter for in vivo interference assay.DepositorInsertCas12c2
UseTagsExpressionBacterialMutationPromoterAvailable sinceJune 2, 2022AvailabilityAcademic Institutions and Nonprofits only -
A0A075HNX4
Plasmid#177695Purposeexpresses a fatty alcohol oxidase in E. coliDepositorInsertLong chain alcohol oxidase
UseTags10xHisExpressionBacterialMutationPromoterT7Available sinceDec. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET28-OmpT
Plasmid#68862Purposeconstruct for expression of OmpT protease in the pET28a plasmid using the restriction sites NcoI and EcoRI.DepositorInsertOmpT (ompT )
UseTags6xHisExpressionBacterialMutationPromoterAvailable sinceOct. 9, 2015AvailabilityAcademic Institutions and Nonprofits only -
pAV10.KN
Plasmid#63209PurposepCACS backbone (Amp), cloNAT, contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for TU assembly using yGG. Integration into YKL162C-A (NotI).DepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionBacterial and YeastMutationPromoterAvailable sinceApril 28, 2017AvailabilityAcademic Institutions and Nonprofits only -
pET11a+CYP119
Plasmid#66131PurposeExpresses the wild type of CYP119DepositorInsertCYP119 (cyp119 )
UseTagsExpressionBacterialMutationPromoterT7 promoterAvailable sinceJune 29, 2015AvailabilityAcademic Institutions and Nonprofits only