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Plasmid#111618PurposeFluorescent biosensor for gauging the activity of the PQS quorum sensing system in Pseudomonas aeruginosaDepositorInsertPpqsA-gfp(ASV)
UseTagsExpressionBacterialMutationPromoterAvailable sinceJune 25, 2018AvailabilityAcademic Institutions and Nonprofits only -
pEXP5-NT/pLux eGFP
Plasmid#193624PurposeInducible expression of eGFP by the transcription factor complex LuxR-3OC6 homoserine lactone (HSL).DepositorInserteGFP
UseTags6xHisExpressionBacterialMutationPromoterpLuxAvailable sinceJuly 18, 2023AvailabilityAcademic Institutions and Nonprofits only -
pEXP5-NT/pT7 LuxR
Plasmid#193625PurposeConstitutive T7 RNAP-mediated expression of LuxR transcription factor for Lux quorum sensing system. LuxR sequence is codon optimized for E. coli.DepositorInsertLuxR
UseTags6xHisExpressionBacterialMutationPromoterT7 RNAP promoterAvailable sinceJuly 18, 2023AvailabilityAcademic Institutions and Nonprofits only -
pNCST-mAvicFP1
Plasmid#129509PurposeExpresses mAvicFP1 constitutively in E. coli (most strains)DepositorInsertmAvicFP1
UseTagsExpressionBacterialMutationPromotersynthetic constitutive (stationary phase) promote…Available sinceFeb. 19, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGEX-2TK cyclin T1 (1-300) (P#432)
Plasmid#14608DepositorInsertcyclin T1 (1-300) (CCNT1 Human)
UseTagsGSTExpressionBacterialMutationamino acids 1-300PromoterAvailable sinceJune 30, 2008AvailabilityAcademic Institutions and Nonprofits only -
pSNAP-CLC-SNAP
Plasmid#38012DepositorInsertClathrin, light polypeptide (LCa) (Clta Mouse)
UseTagsSNAP26mExpressionBacterial and MammalianMutationPromoterCMVAvailable sinceSept. 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
pJMP1223
Plasmid#119261Purposerfp "test" strainDepositorInsertdcas9
UseTagsExpressionBacterialMutationD10A and H840APromoterAvailable sinceJan. 7, 2019AvailabilityAcademic Institutions and Nonprofits only -
pJMP1221
Plasmid#119260Purposerfp "test" strainDepositorInsertsgRNA NT1/RR1 (rfp)
UseTagsExpressionBacterialMutationD10A and H840APromoterAvailable sinceJan. 7, 2019AvailabilityAcademic Institutions and Nonprofits only -
pUS252b
Plasmid#191831PurposeExpresses fuGFPb constitutively in E.coli cells. Designed to act as modular template for cutting out or amplifying fuGFPb to place in other plasmid backbonesDepositorInsertfuGFPb
UseTagsExpressionBacterialMutationPromoterAvailable sinceJan. 17, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits -
CaP2AIII_pK18
Plasmid#153421PurposeSource of murine TCR-alpha constant region (TRAC*02) gene followed by P2ADepositorInsertmurine TCR-alpha constant region (TRAC*02)-P2A
UseTagsExpressionBacterialMutationPromoterAvailable sinceAug. 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBMT-4
Plasmid#22837DepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceJan. 28, 2010AvailabilityAcademic Institutions and Nonprofits only -
pCC1-4k-BBclone2
Plasmid#137070PurposeE. coli plasmid that contains the expression optimized genes for BB0323, P13, DipA and Lmp1DepositorInsertsBB0323
P13
DipA
Lmp1
UseTagsExpressionBacterialMutationwild-type, full-length (signal-peptide-containing…PromoterAvailable sinceMay 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
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pH
Plasmid#64178Purposeharbours marker gene HIS3DepositorTypeEmpty backboneUseTagsExpressionBacterial and YeastMutationPromoterAvailable sinceSept. 20, 2016AvailabilityAcademic Institutions and Nonprofits only -
pET15b I-SceI (Ctermhis -MH)
Plasmid#195613PurposeExpresses the wild-type I-SceI homing endonuclease from the T7 promoterDepositorInsertI-SceI
UseTagsExpressionBacterialMutationNonePromoterT7Available sinceFeb. 1, 2023AvailabilityAcademic Institutions and Nonprofits only -
pReporter_5
Plasmid#60566PurposeA reporter plasmid containing the attB and attP sites flanking a green fluorescent protein reporter gene (gfp).DepositorInsertReporter_5
UseTagsExpressionBacterialMutationPromoterBBa_J23119Available sinceMarch 16, 2015AvailabilityAcademic Institutions and Nonprofits only -
FGFR1_HUMAN_D0
Plasmid#79719PurposeThis plasmid encodes the kinase domain of FGFR1. Intended for co-expression with YopH Phosphatase that accompanies this set to enhance bacterial kinase expression.DepositorInsertFGFR1 (FGFR1 Human)
UseTagsHis10-TEVExpressionBacterialMutationPromoterT7Available sinceNov. 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
pBLxR5
Plasmid#167504PurposeBroad-Host range expression vector, pBBR origin, LuxR activator, PLuxB promoter, mRFP, Gentamicin Resistance Marker.DepositorInsertLuxR-mRFP
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterPLuxBAvailable sinceOct. 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
pRDA_791
Plasmid#216079PurposeMinimal plasmid; contains only ori and ampR, destination vectorDepositorHas ServiceCloning Grade DNATypeEmpty backboneUseDestinationTagsExpressionBacterialMutationPromoterAvailable sinceMay 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pOPINVHH_cys_his
Plasmid#210403PurposeFor expression of VHH with C-terminal cysteine and his tagDepositorTypeEmpty backboneUseTagscys-his6ExpressionBacterialMutationPromoterlacAvailable sinceMarch 26, 2024AvailabilityAcademic Institutions and Nonprofits only