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Plasmid#39316DepositorInsertCas9
UseCRISPRTagsHis6 and MBPExpressionBacterialMutationHistidine 840 to Alanine (H840A)PromoterT7Available sinceAug. 28, 2012AvailabilityAcademic Institutions and Nonprofits only -
pMBP-SpyCatcher
Plasmid#79053PurposeExpresses maltose binding protein (MBP) SpyCatcher fusionDepositorInsertMBP-SpyCatcher
UseTags6xHis and Maltose binding proteinExpressionBacterialMutationDerived from pDEST14-SpyCatcher (Plasmid #35044)…PromoterT7Available sinceJune 15, 2016AvailabilityAcademic Institutions and Nonprofits only -
p36-p37-p38-p40-pET-Duet-1
Plasmid#175049PurposeOverexpression of human Rfc2,Rfc3,Rfc4,Rfc5 in E.coliDepositorUseTagsExpressionBacterialMutationPromoterT7 promoterAvailable sinceSept. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pH3U3-mcs
Plasmid#12609DepositorInsertMultiple cloning site in His3 Ura3 reporter plasmid
UseTagsExpressionBacterialMutationPromoterAvailable sinceSept. 26, 2006AvailabilityAcademic Institutions and Nonprofits only -
pCyPet-His
Plasmid#14030DepositorInsertCyPet
UseTags6xHisExpressionBacterialMutationPromoterAvailable sinceFeb. 14, 2007AvailabilityAcademic Institutions and Nonprofits only -
pET22b-6h-GST-TEVp
Plasmid#172887PurposeCodon optimized (E. coli) TEVp fused with GST and his-tagged for purificationDepositorInsertTEV protease
UseTagsGST solubility tag and HHHHHHExpressionBacterialMutationPromoterT7 promoterAvailable sinceJuly 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
DP6
Plasmid#140446PurposeUntargetted bacterial driftDepositorInsertdnaQ926 dam seqA emrR ugi CDA1 gIII
UseTagsExpressionBacterialMutationPromoterAvailable sinceApril 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
pSANG10-3F-BG4
Plasmid#55756PurposeExpresses BG4 scFv anti-G-quadruplex antibody in bacteriaDepositorInsertBG4
UseTags6xHis, pelB leader, and tri-FLAGExpressionBacterialMutationPromoterT7Available sinceAug. 11, 2014AvailabilityAcademic Institutions and Nonprofits only -
pH6HTC_YBX3-Halo
Plasmid#175308PurposeProduction of HaloTag fusion proteinDepositorInsertYBX3 (YBX3 Human)
UseCell free expressionTagsHaloTag-6xHisExpressionBacterialMutationPromoterAvailable sinceOct. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pRSET iGluSnFR3 v857
Plasmid#175186PurposeFluorescent reporter for glutamate, third generation, variant 857 soluble form to express in bacteria.DepositorInsertiGluSnFR3 v857
UseSynthetic BiologyTagsHis tag, T7 leader, Xpress epitopeExpressionBacterialMutationPromoterT7Available sinceSept. 28, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBbS5a-Opto-Cre-Vvd-2
Plasmid#160400PurposeUpdated light-inducible Cre recombinase split with Vivid photodimers for bacterial expression.DepositorInsertOpto-Cre-Vvd-2
UseCre/Lox and Synthetic BiologyTagsExpressionBacterialMutationPromoterlacUV5Available sinceOct. 20, 2020AvailabilityAcademic Institutions and Nonprofits only -
pEMG-AIS
Plasmid#225133PurposeFor genome editing in P. putida KT2440, arabinose is used for the induction of I-sceI gene. Km.DepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceOct. 10, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
pEEK2
Plasmid#83492PurposeExpression plasmid for conjugational transfer into cyanobacteriaDepositorTypeEmpty backboneUseSynthetic BiologyTagsStrep-tagExpressionBacterialMutationPromoterPtrc coreAvailable sinceNov. 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
pGEX-human p53-(1-393)
Plasmid#24860DepositorInsertp53 (TP53 Human)
UseTagsGSTExpressionBacterialMutationPromoterAvailable sinceAug. 5, 2010AvailabilityAcademic Institutions and Nonprofits only -
LentiGuide Puro-P2A-EGFP
Plasmid#137729PurposeFor simple determination of the multiplicity of infection (MOI) of a lentiviral CRISPR library by checking EGFP expression (still allowing for puro selection).DepositorTypeEmpty backboneUseCRISPR, Lentiviral, and Synthetic BiologyTagsEGFPExpressionBacterial and MammalianMutationPromoterAvailable sinceFeb. 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
3xFLAG-CUL4B-pCMV7.1
Plasmid#155023PurposeN-terminal 3xFLAG-tagged CUL4B in a pCMV7.1 vectorDepositorInsertCullin 4B (CUL4B Human)
UseTags3xFLAGExpressionBacterialMutationPromoterpCMV7.1Available sinceSept. 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMAL-p2X
Plasmid#75287PurposeExpresses proteins in the periplasm as fusions to maltose-binding proteinDepositorTypeEmpty backboneUseTagsmaltose-binding proteinExpressionBacterialMutationPromotertacAvailable sinceJuly 18, 2016AvailabilityIndustry, Academic Institutions, and Nonprofits -
pQCH
Plasmid#164160PurposeIncQ BHR plasmidDepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceFeb. 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
pRSET YBX-1
Plasmid#13035DepositorInsertYBX1 (YBX1 Human)
UseTags6xHisExpressionBacterialMutationPromoterAvailable sinceOct. 5, 2006AvailabilityAcademic Institutions and Nonprofits only -