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Plasmid#16575DepositorInsertBAX knockout (BAX Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceApril 7, 2008AvailabilityAcademic Institutions and Nonprofits only -
pBBRBB-Ppuf843-1200-PR
Plasmid#50963PurposeExpresses proteorhodopsin using the Ppuf843-1200 promoter segment, for use in R. sphaeroidesDepositorInsertProteorhodopsin
UseSynthetic BiologyTags6xHisExpressionBacterialMutationPromoterPpuf843-1200Available sinceFeb. 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
pTriEx-mCherry-Zdk1-Rac1 Q61L
Plasmid#81059Purposemammalian expression of Zdk1 tagged Rac1 Q61LDepositorInsertsUseTagsmCherryExpressionBacterial, Insect, and Mamm…MutationPromoterAvailable sinceAug. 30, 2016AvailabilityAcademic Institutions and Nonprofits only -
pACYC-PLlambda-alkS(WT)
Plasmid#44382DepositorInsertAlkS(WT)
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterPLlambdaAvailable sinceJuly 15, 2013AvailabilityIndustry, Academic Institutions, and Nonprofits -
pET28_mouse-Caspase-11_enzymatic-domain
Plasmid#183382PurposeOverexpression of catalytic domain of inflammatory caspase in bacteriaDepositorInsertCaspase-11 (AA100-373) (Casp4 Mouse)
UseTagsHis-TEVExpressionBacterialMutationPromoterT7Available sinceJuly 18, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAB094a
Plasmid#79241PurposeDirected Evolution of Protein-Protein InteractionsDepositorInsertPBAD rpoZ-HA4; PC araC
UseTagsExpressionBacterialMutationPromoterPBAD; PCAvailable sinceApril 21, 2017AvailabilityAcademic Institutions and Nonprofits only -
pBAD-HisD-miRFP713-HO1
Plasmid#197255PurposeExpresses the protein of miRFP713 and HO1 in bacteriaDepositorInsertmiRFP713
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMAL658
Plasmid#167837PurposeEncodes OptoLAC1B circuitDepositorInsertlacI
UseSynthetic BiologyTagsssrA (LAA)ExpressionBacterialMutationPromoterAvailable sinceSept. 28, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMAL659
Plasmid#167838PurposeEncodes OptoLAC2B circuitDepositorInsertlacI
UseSynthetic BiologyTagsssrA (AAV)ExpressionBacterialMutationPromoterAvailable sinceSept. 28, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET-SUMO mCER-Prx2
Plasmid#158226PurposeGenetically encoded HomoFRET-based probe to monitor peroxiredoxin2 (Prx2) oligomerization (Recombinant bacterial expression)DepositorInsertmCER-Prx2
UseTagsExpressionBacterialMutationPromoterAvailable sinceMay 28, 2021AvailabilityAcademic Institutions and Nonprofits only -
pKL12-GCaMP6f-pHuji bb118
Plasmid#158981PurposeConstitutive expression (sigma 70 promoter) of GCaMP6f tethered to pHuji fluorophores in E. coli.DepositorInsertGCaMP6f-pHuji
UseTagsExpressionBacterialMutationcodon optimized for e. coli from Addgene#100833 a…PromoterBba_J23118 (sigma 70)Available sinceSept. 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
TOPO Cre-2A-GFP
Plasmid#68450PurposeTOPO construct expressing Cre-2A-GFP for generation of GMAP-compatible geneDepositorInsertCre-2A-GFP
UseGmapTagsExpressionBacterialMutationPromoterPlacAvailable sinceFeb. 22, 2016AvailabilityAcademic Institutions and Nonprofits only -
pPBO.ACT005
Plasmid#182715PurposeConstituitvely expressed CasRx crRNA cloning vector, truncated 3' terminator regionDepositorInsertCasRx crRNA cloning backbone
UseCRISPRTagsExpressionBacterialMutationCatalytically deactivated R295A, H300A, R849A, H8…PromoterpJ23119 (TTGACAGCTAGCTCAGTCCTAGGTATAATACTAGT)Available sinceAug. 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
pFGL821
Plasmid#58223PurposeFor fungal transformation via ATMT. Transformants to be selected by hygromicin resistance.DepositorInserthygromycin B phosphotransferase
UseTagsExpressionBacterialMutationPromoterAspergillus nidulans trpC promoterAvailable sinceMay 26, 2015AvailabilityAcademic Institutions and Nonprofits only -
5VBD (USP09X)
Plasmid#101260PurposeBacterial expression for structure determination; may not be full ORFDepositorInsertUSP09X (USP9X Human)
UseTags6xHis-TEVExpressionBacterialMutationPromoterT7Available sinceOct. 2, 2017AvailabilityIndustry, Academic Institutions, and Nonprofits -
pET28a-PSAT
Plasmid#74115PurposeExpression of PSATDepositorInsertpET28a-PSAT (PSAT1 Human)
UseTagsExpressionBacterial and MammalianMutationPromoterAvailable sinceMarch 22, 2019AvailabilityAcademic Institutions and Nonprofits only -
RP1B-PP1alpha
Plasmid#51768PurposeExpresses PP1 alpha residues 7-330DepositorInsertpp1 alpha
UseTagsHis, TEV protease site, and Thio-6ExpressionBacterialMutationContains PP1 alpha residues 7-330PromoterT7-lacAvailable sinceMarch 25, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCas9-beta-mmr
Plasmid#169241PurposeCas9, beta, and mutL mutant expression plasmid for introducing chromosomal point mutationsDepositorInsertscas9
beta
mutL-E36K
UseCRISPR and Synthetic BiologyTagsExpressionBacterialMutationChanged glutamic acid 36 to lysinePromoterAvailable sinceJune 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
Grp1-PH pGEX2TK
Plasmid#71379Purposebacterial expression of PH domain for phosphoinositide binding assayDepositorInsertGrp1-PH (Cyth3 Mouse)
UseTagsGSTExpressionBacterialMutationPleckstrin homology (PH) domain; aa 264-391PromotertacAvailable sinceFeb. 26, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAKgfp2
Plasmid#14077DepositorInsertlacIq repressor gene
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 12, 2007AvailabilityAcademic Institutions and Nonprofits only