-
Plasmid#22242DepositorInsertEpsin 2 (EPN2 Human)
UseTagsGFPExpressionBacterial and MammalianMutationPromoterAvailable sinceDec. 3, 2009AvailabilityAcademic Institutions and Nonprofits only -
pET23 P53DBD
Plasmid#174503Purposeexpress P53 DNA binding domain (94-312) in E.coli without tagDepositorInsertP53 DNA binding domain (corresponds to amino acid 94-312) (TP53 Human)
UseTagsnoneExpressionBacterialMutationPromoterT7Available sinceOct. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET15b-sfGO-Matryoshka-LE-LP
Plasmid#100018PurposeBacterial expression of fluorescent protein casette - cpsfGFP with nested LSSmOrange and LE N-term and LP C-term amino acid linkersDepositorInsertcpsfGFP-LSSmOrange
UseTags6x HIS tagExpressionBacterialMutationLeu and Glu N-term linker and Leu and Pro C-term …PromoterT7Available sinceOct. 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
pBAD-Ag43
Plasmid#107743PurposeExpresses Ag43 under the control of an L-arabinose transcriptional regulatorDepositorInsertAg43 (flu )
UseTagsExpressionBacterialMutationremoved all pstI cut sites with silent mutations,…PromoterpBADAvailable sinceMay 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
MiCy-pBAD
Plasmid#54565PurposeLocalization: Protein Expression Vector , Excitation: 472, Emission: 495DepositorTypeEmpty backboneUseTags6xHis and MiCyExpressionBacterialMutationPromoterAvailable sinceJuly 25, 2014AvailabilityAcademic Institutions and Nonprofits only -
pOPS0750
Plasmid#115511PurposeReporter plasmid suitable for experiments in environments where there is no antibiotic selection present. Constitutive promoter pNeo expressing gusA.DepositorInsertgusA pOGG083, T-pharma pOGG003 assembled in pOGG026
UseTagsExpressionBacterialMutationDomesticated for Golden Gate cloningPromoterConstitutive promoter pNeo pOGG001Available sinceJan. 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
pUC18T-mini-Tn7T-Gm-Pc-mNeonGreen
Plasmid#205022Purposemini-Tn7 vector for insertion of mNeonGreen (codon optimized for P. fluorescens) at bacterial attTn7 siteDepositorInsertmNeonGreen
UseTagsExpressionBacterialMutationmNeonGreen is codon optimized for expression in P…PromoterPcAvailable sinceSept. 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET28_mouse-Caspase-1_enzymatic-domain
Plasmid#183385PurposeOverexpression of catalytic domain of inflammatory caspase in bacteriaDepositorInsertCaspase-1 (AA130-404) (Casp1 Mouse)
UseTagsHis-TEVExpressionBacterialMutationPromoterT7Available sinceMay 5, 2022AvailabilityAcademic Institutions and Nonprofits only -
pmCellFree_KA1_ZIKV NS2A
Plasmid#145343PurposeCell-free/mammalian expression vector for Zika virus NS2A protein with N-term eGFPDepositorInsertZIKV NS2A
UseTags8xHis and eGFPExpressionBacterialMutationPromoterT7- CMV/CAGAvailable sinceFeb. 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMRE-Tn7-161
Plasmid#118573PurposeminiTn7 plasmid to deliver constitutively expressed fluorescent protein genes in bacteriaDepositorInsertmTurquoise2
UseMinitn7 delivery vectorTagsExpressionBacterialMutationPromoterAvailable sinceOct. 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
pKP864
Plasmid#167658PurposeExpress colicinB-His6DepositorInsertcba-his6
UseTagsExpressionBacterialMutationcba-his6PromoterAvailable sinceJuly 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
AAK1
Plasmid#73244PurposeBacterial expression for structure determination; may not be full ORFDepositorInsertAAK1 (AAK1 Human)
UseTagsTEV-His6-FlagExpressionBacterialMutationpfam00069:Pkinase 47-307PromoterT7Available sinceApril 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
pET15-EF4n_mFAP2b
Plasmid#159464PurposeEncodes for EF4n_mFAP2b with an N-terminal 6xHis tag, thrombin epitope, and TEV epitope. Expression is controlled by the T7 promoter with E. coli codon optimization.DepositorInsertEF4n_mFAP2b
UseTags6xHis-thrombin-TEVExpressionBacterialMutationPromoterAvailable sinceNov. 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
pHnCBS1D
Plasmid#52065Purposeexpresses nine genes from the H. neapolitanus carboxysome operon and CSOS1D; IPTG-inducibleDepositorInsertCbbL, CbbS, CSOS2, CSOS3, CSOS4A, CSOS4B, CSOS1C, CSOS1A, CSOS1B, CSOS1D
UseTagsExpressionBacterialMutationPromoterAvailable sinceApril 4, 2014AvailabilityAcademic Institutions and Nonprofits only -
pET_scFv_QLuc
Plasmid#183261PurposeExpress scFv_QLuc in bacteria cellsDepositorInsertscFv_QLuc
UseTagsHistagExpressionBacterialMutationPromoterT7Available sinceAug. 2, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET-3c-rhVTN-NC
Plasmid#30226DepositorInsertrhVitronectin with N and C terminus cleavages (VTN Human)
UseTagsExpressionBacterialMutationdeleted amino acids: 399-478, 20-62PromoterAvailable sinceSept. 26, 2011AvailabilityAcademic Institutions and Nonprofits only -
PAJ698
Plasmid#178947PurposeExpresses P2 tail fiber (gpH) in fusion with S16 tail fiber (gp37)DepositorInsertChimeric Tail Fiber Fusion P2-gpH and S16 gp37
UseUnspecifiedTagsExpressionBacterialMutationPromoterAvailable sinceNov. 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
pGT-Ah5
Plasmid#122572PurposeMAGIC donor plasmid (Himar transposon with catP/sfGFP payload)DepositorInsertcatP/sfGFP
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceMay 7, 2019AvailabilityAcademic Institutions and Nonprofits only -
-
pDOC-K-glmS-GFPfor
Plasmid#158059PurposeDonor plasmid for insertion of eGFP and the constitutive acpP promoter into the S. Typhimurium chromosome. GFP is in the forward orientation relative to the kanamycin resistance selectable markerDepositorInsertGreen Fluorescent Protein optimised for excitation with UV light
UseSynthetic BiologyTagsExpressionBacterialMutationPromoteracpPAvailable sinceSept. 15, 2020AvailabilityAcademic Institutions and Nonprofits only