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pET28b-Ava3855

Plasmid

#45743

Depositor

Christopher T Walsh, Emily Balskus

Article

Balskus et al Science. 2010 Sep 24;329(5999):1653-6. d

Insert

Ava3855 (Ava_3855 Anabaena variabilis)

Use

Tags

His

Expression

Bacterial

Mutation

Promoter

T7

Available since

June 3, 2013

Availability

Academic Institutions and Nonprofits only
pDEST17-UbE2E3

Plasmid

#15789

Depositor

Wade Harper

Article

Jin et al Nature. 2007 Jun 28. 447(7148):1135-8.

Insert

Ubiquitin conjugating enzyme UbE2E3 (UBE2E3 Human)

Use

Tags

His

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 28, 2007

Availability

Academic Institutions and Nonprofits only

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pZS2-123

Plasmid

#26598

Depositor

Michael Elowitz

Article

Cox et al J Biol Eng. 2010 . 4():10.

Insert

Synthetic

Use

Tags

Expression

Bacterial

Mutation

N/A

Promoter

Available since

Feb. 23, 2011

Availability

Academic Institutions and Nonprofits only

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pMUT2-pCaf-mCherry

Plasmid

#192860

Purpose

pMUT2 based vector for sensing caffeine in E. coli Nissle 1917

Depositor

Morten Sommer

Article

Vaaben et al ACS Synth Biol. 2022 Dec 16;11(12):4184-

Insert

Nanobody-CadC fusion protein and cognate promoter inducible by caffeine

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Aug. 7, 2023

Availability

Academic Institutions and Nonprofits only

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HC89 pComb3xss anti-Homer1 nanobody

Plasmid

#134719

Purpose

Bacterial expression of anti-Homer1 (Mouse) recombinant llama nanobody for use as an immunolabel

Depositor

James Trimmer

Article

Dong et al Elife. 2019 Sep 30;8. pii: 48750. doi: 1

Insert

anti-Homer1 (Mouse) recombinant llama nanobody HC89 (Homer1 L. glama (llama))

Use

Tags

6xHis-HA

Expression

Bacterial

Mutation

Promoter

lac

Available since

Dec. 10, 2019

Availability

Academic Institutions and Nonprofits only

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pBAD-O-GECO1

Plasmid

#113938

Purpose

Bacterial expression of orange fluorescent Ca2+ sensor O-GECO1

Depositor

Robert Campbell, Thomas Hughes

Article

Molina et al Biophys J. 2019 May 21;116(10):1873-1886

Insert

O-GECO1

Use

Tags

6xHis

Expression

Bacterial

Mutation

Promoter

Available since

Dec. 3, 2018

Availability

Academic Institutions and Nonprofits only

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Rb-379-928-pGex2TK

Plasmid

#21749

Depositor

William Kaelin

Article

Kaelin et al Cell. 1992 Jul 24. 70(2):351-64.

Insert

Rb (RB1 Human)

Use

Tags

GST

Expression

Bacterial

Mutation

deletion mutant ORF contains aa 379-928 of wt Rb …

Promoter

Available since

Oct. 5, 2009

Availability

Academic Institutions and Nonprofits only

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p_SNIPR_target_WT

Plasmid

#139465

Purpose

T7 RNAP-driven expression of the cognate SNIPR trigger RNA that is complementary to the SNIPR RNA sensor

Depositor

Alexander Green

Article

Hong et al Cell. 2020 Feb 25. pii: S0092-8674(20)30

Insert

Target_WT

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 19, 2020

Availability

Academic Institutions and Nonprofits only

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pTH278-GST-TEV-SUP45

Plasmid

#29743

Depositor

Tobias von der Haar

Insert

SUP45 gene encoding translation release factor 1 from S. cerevisiae (SUP45 Budding Yeast)

Use

Tags

GST

Expression

Bacterial

Mutation

wild type

Promoter

Available since

Sept. 6, 2011

Availability

Academic Institutions and Nonprofits only

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pET28a-mH6-Cas12g1

Plasmid

#120879

Purpose

Expresses E. coli codon optimized Cas12g1 effector in the pET28a backbone.

Depositor

Arbor Biotechnologies

Article

Yan et al Science. 2019 Jan 4;363(6422):88-91. doi

Insert

Cas12g1

Use

CRISPR

Tags

mH6

Expression

Bacterial

Mutation

Promoter

lac

Available since

Feb. 14, 2019

Availability

Academic Institutions and Nonprofits only

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pSF-p15A-YFP

Plasmid

#186563

Purpose

p15A ori, promotorless, AmpR. Kringle YFP (Yellow Fluorescence Protein) expression

Depositor

Teresa de Diego Puente

Article

Lozano Terol et al Front Microbiol. 2021 Jun 21;12:682001.

Insert

YFP

Use

Tags

None

Expression

Bacterial and Mammalian

Mutation

None

Promoter

Promotorless

Available since

Nov. 29, 2022

Availability

Academic Institutions and Nonprofits only

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pUC19-T7-Bottom-T

Plasmid

#87310

Purpose

Expresses the "Bottom" transcript of the Split-Broccoli system under the control of the T7 promoter.

Depositor

Donald Burke

Article

Alam et al ACS Synth Biol. 2017 May 26. doi: 10.102

Insert

Split-Broccoli system "Bottom" transcript

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

T7

Available since

July 27, 2018

Availability

Academic Institutions and Nonprofits only

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pQLinkHD_EBFP2-LSSmOrange

Plasmid

#118856

Purpose

fluorescent protein expression with an N-terminal His-tag

Depositor

Marc Birtwistle

Article

Holzapfel et al ACS Comb Sci. 2018 Nov 12;20(11):653-659

Insert

EBFP2-LSSmOrange

Use

Tags

His-tag

Expression

Bacterial

Mutation

EBFP1.2-I128V/V150I/ D155V/V224R and for LSSmOra…

Promoter

Available since

Dec. 12, 2018

Availability

Academic Institutions and Nonprofits only

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XRCC1-c003

Plasmid

#173090

Purpose

Expression of recombinant fusion protein in E. coli

Depositor

Lari Lehtiö

Article

Sowa et al Cell Rep Methods. 2021 Dec 20;1(8):10012

Insert

XRCC1 (XRCC1 Human)

Use

Tags

mCerulean

Expression

Bacterial

Mutation

E315-P406

Promoter

Available since

Dec. 9, 2021

Availability

Academic Institutions and Nonprofits only

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pET19b_4CL + STS

Plasmid

#139790

Purpose

expression of biosynthetic enzymes 4CL and STS

Depositor

Roman Jerala

Article

Fink et al AMB Express. 2020 May 24;10(1):97. doi:

Inserts

4CL
STS

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Aug. 19, 2020

Availability

Academic Institutions and Nonprofits only

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pYC1876

Plasmid

#158722

Purpose

Helper plasmid pYC1640 for C-terminal tagging with degradation tag ssrA

Depositor

Yi-Cheng Sun

Article

Yan et al mBio. 2020 Jan 28;11(1). pii: mBio.02364

Insert

ssrA

Use

CRISPR

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Jan. 15, 2021

Availability

Academic Institutions and Nonprofits only

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pJAK2.D

Plasmid

#29465

Depositor

Zakaria Hmama

Article

Sun et al Plasmid. 2009 Nov . 62(3):158-65.

Type

Empty backbone

Use

Mycobacteria expression

Tags

6xHis

Expression

Bacterial

Mutation

Promoter

Acetamide promoter

Available since

March 31, 2011

Availability

Academic Institutions and Nonprofits only

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pBA2257

Plasmid

#38151

Depositor

David Waugh

Article

Austin et al Protein Expr Purif. 2012 Mar;82(1):116-2

Insert

MBP-proMeCPA fusion protein

Use

Tags

maltose binding protein

Expression

Bacterial

Mutation

Promoter

tac

Available since

Aug. 21, 2012

Availability

Academic Institutions and Nonprofits only

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pKJ1267 (aka pAC-alphaGal4)

Plasmid

#21869

Depositor

Keith Joung

Article

Maeder et al Nat Protoc. 2009. 4(10):1471-501.

Type

Empty backbone

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Nov. 3, 2009

Availability

Academic Institutions and Nonprofits only

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pH6HTC_IGF2BP1-Halo

Plasmid

#175300

Purpose

Production of HaloTag fusion protein

Depositor

Claudia Kutter

Article

Atanasoai et al bioRxiv 2021.11.08.467743

Insert

IGF2BP1 (IGF2BP1 Human)

Use

Cell free expression

Tags

HaloTag-6xHis

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 7, 2021

Availability

Academic Institutions and Nonprofits only

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