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Showing: 7021 - 7040 of 28470 results

  4. pES134

    Plasmid
    #127551
    Purpose
    Variant of pKD13. Lambda Red recombineering PCR template plasmid carrying an F3-aadA-F3 cassette orthogonal to FRT-KanR-FRT in pKD13
    Depositor
    Emmanuele Severi, Gavin Thomas
    Article
    Wu et al Microbiology (Reading). 2024 Mar;170(3).
    Insert
    F3-flanked spectinomycin resistance cassette
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    July 29, 2019
    Availability
    Academic Institutions and Nonprofits only
    127551-plasmid-map-sequence-id-251734

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  5. RG-001

    Plasmid
    #133439
    Purpose
    Heat 3 domain of human elF4G in pET21 backbone.
    Depositor
    Gerhard Wagner
    Insert
    EIF4G1 (EIF4G1 Human)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Jan. 22, 2020
    Availability
    Academic Institutions and Nonprofits only
    133439-plasmid-map-sequence-id-269573

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  6. pTriEx-mVenus-PA-Rac1-T17N

    Plasmid
    #22017
    Purpose
    Expression of photoactivatable Rac1 (dominant-negative mutant)
    Depositor
    Klaus Hahn
    Article
    Wu et al Nature. 2009 Sep 3. 461(7260):104-8.
    Insert
    PA-Rac1-T17N (RAC1 Human, Avena sativa (oat))
    Use
    Tags
    6X His and mVenus
    Expression
    Bacterial, Insect, and Mamm…
    Mutation
    Rac1 starts at I4 and contains mutation T17N.
    Promoter
    CMV, p10
    Available since
    Sept. 29, 2009
    Availability
    Academic Institutions and Nonprofits only
    22017-plasmid-map-sequence-id-138512

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  8. pDule2-IBBN (G2)

    Plasmid
    #85501
    Purpose
    Plasmid for incorporating the non-canonical amino acid IBBN with the Mj IBBN (G2) synthetase and cognate amber suppressing tRNA in Ecoli.
    Depositor
    Ryan Mehl
    Article
    Peeler et al J Am Chem Soc. 2010 Oct 6;132(39):13575-
    Insert
    IBBN (G2) synthetase and cognate amber suppressing tRNA derived from M. jannaschii Tyrosine synthetase/tRNA system
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Y32G L65E F108W Q109M D158S R162K
    Promoter
    lpp (constitutive)
    Available since
    March 2, 2017
    Availability
    Academic Institutions and Nonprofits only
    85501-plasmid-map-sequence-id-169858

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  9. PCRT7 tetR pLtetO MBP-MEK1 XX Amp

    Plasmid
    #53225
    Purpose
    human MEK1 with MBP and HIS tags, with Ser218 and Ser222 changed to amber codons, for use in phosphoprotein synthesis
    Depositor
    Jesse Rinehart
    Insert
    MEK1 (MAP2K1 Human)
    Use
    Phosphoprotein synthesis
    Tags
    HIS and MBP
    Expression
    Bacterial
    Mutation
    Codon optimized for E.coli, both codons for Ser21…
    Promoter
    pLtetO
    Available since
    May 7, 2014
    Availability
    Academic Institutions and Nonprofits only
    53225-plasmid-map-sequence-id-82901

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  10. pGBK CUP1 EGFP

    Plasmid
    #170210
    Purpose
    Yeast two-hybrid vector using a copper inducible CUP1 promoter, expressing EGFP
    Depositor
    Bruce Hamilton
    Article
    Hernandez et al bioRxiv
    Insert
    enhanced green fluorescent protein
    Use
    Tags
    GAL4 DNA Binding Domain and Myc
    Expression
    Bacterial and Yeast
    Mutation
    Promoter
    CUP1
    Available since
    July 6, 2021
    Availability
    Academic Institutions and Nonprofits only
    170210-plasmid-map-sequence-id-335104

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  15. PTPN5

    Plasmid
    #39166
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    PTPN5 (PTPN5 Human)
    Use
    Tags
    His Tev
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    March 18, 2013
    Availability
    Academic Institutions and Nonprofits only

  20. MDH2

    Plasmid
    #38792
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    MDH2 (MDH2 Human)
    Use
    Tags
    His Tev
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    March 18, 2013
    Availability
    Academic Institutions and Nonprofits only
Showing: 7021 - 7040 of 28470 results