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Plasmid#131645PurposeBacterial expression of ETS gene ETV4DepositorInsertETV4 (ETV4 Human)
UseTags6xHisExpressionBacterialMutationPromoterAvailable sinceOct. 3, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET22B_PcrA_2Bchimera
Plasmid#102998PurposeExpress a DNA helicase, a chimeric construct of Bacilllus stearothermophilus PcrA and 2B domain of Staphylococcus aureus PcrADepositorInsertPcrA_2Bchimera
UseTagsExpressionBacterialMutationBacilllus stearothermophilus PcrA in which the 2B…PromoterT7Available sinceMay 24, 2018AvailabilityAcademic Institutions and Nonprofits only -
FLAG-Matr3 delZnF1
Plasmid#32883DepositorInsertMATR3 Matrin3 (MATR3 Human)
UseTagsFLAGExpressionBacterial and MammalianMutationdeleted amino acids 283-322PromoterCMVAvailable sinceJan. 6, 2012AvailabilityAcademic Institutions and Nonprofits only -
FLAG-Matr3 delZnF2
Plasmid#32884DepositorInsertMATR3 Matrin3 (MATR3 Human)
UseTagsFLAGExpressionBacterial and MammalianMutationdeleted amino acids 793-833PromoterCMVAvailable sinceJan. 6, 2012AvailabilityAcademic Institutions and Nonprofits only -
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pET-51b(+)_NADP-Snifit
Plasmid#113913PurposePlasmid encoding the bacterial expression of the FRET semi-synthetic biosensor measuring the ratio NADPH/NADP+DepositorInsertNADP-Snifit
UseTagsExpressionBacterialMutationPromoterT7Available sinceSept. 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
pJSC270 - Bacterial expression plasmid for SpCas9 eSpCas9(1.1)-HF1 variant
Plasmid#101217PurposeBacterial expression plasmid for SpCas9 eSpCas9(1.1)-HF1 variantDepositorInsertSpCas9 variant K848A/K1003A/R1060A/N497A/R661A/Q695A/Q926A
UseTags10x His, MBP, and TEV siteExpressionBacterialMutationK848A, K1003A, R1060A, N497A, R661A, Q695A and Q9…PromoterT7Available sinceOct. 25, 2017AvailabilityAcademic Institutions and Nonprofits only -
pEMI91
Plasmid#74888PurposeShuffled 9I27 polyprotein with shuffled codons to allow facile sequencing and unique RE for the 9 cassettes for versatile cloning. See Depositor Comments for more informationDepositorInsertTitin I27 x 9 (TTN Human)
UseTagsHis-tag and Strep-tag IIExpressionBacterialMutationPromoterT7 promoterAvailable sinceJune 13, 2016AvailabilityAcademic Institutions and Nonprofits only -
pET28-At_PRORP2-His
Plasmid#67870Purposebacterial expression of PRORP2 (At2g16650), full coding sequence + C-term. His tagDepositorInsertPRORP2 (PRORP2 Mustard Weed)
UseTagsHisExpressionBacterialMutationPromoterT7Available sinceAug. 14, 2015AvailabilityAcademic Institutions and Nonprofits only -
pET28-MHL: nsp16_SARS-CoV-2
Plasmid#167850PurposeBacterial expression of the SARS-CoV-2 protein nsp16DepositorInsertnsp16
UseTags6xHis-TEVExpressionBacterialMutationPromoterT7Available sinceApril 16, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
pUCP20T-mPlum
Plasmid#78474PurposeBroad range bacterial fluorescence labelingDepositorInsertmPlum
UseTagsExpressionBacterialMutationPromoterAvailable sinceJune 14, 2016AvailabilityAcademic Institutions and Nonprofits only -
p40phox-PX (1-144)
Plasmid#119123PurposeBacterial expression of human phox homology (PX) domain, p40phox-PX (1-144)DepositorInsertp40phox-PX (1-144)
UseTagsGSTExpressionBacterialMutationPromotertacAvailable sinceOct. 13, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET-PLS-KP6_rpoA-U
Plasmid#190967PurposeTest the U-to-C editing activity of KP6 on AtrpoA editing site, in E. coliDepositorInsertPLS-KP6
UseTagsHisExpressionBacterialMutationPromoterT7Available sinceOct. 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDrDERusk
Plasmid#213703PurposeVector for red-light-induced bacterial gene expressionDepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceMay 30, 2024AvailabilityAcademic Institutions and Nonprofits only -
pTSAR3.5t
Plasmid#210257PurposeFluorescent reporter for Type III secretion system activity in Shigella flexneriDepositorInsertseBFP2
superfolder GFP
UseTagsssraExpressionBacterialMutationPromoteripaH7.8p and rpsMAvailable sinceFeb. 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
pTSAR3.4t
Plasmid#210256PurposeFluorescent reporter for Type III secretion system activity in Shigella flexneriDepositorInsertsmCherry
superfolder GFP
UseTagsssraExpressionBacterialMutationPromoteripaH7.8p and rpsMAvailable sinceFeb. 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGEX-6P-1_WT RAD23A
Plasmid#201456PurposeExpresses human RAD23A with an N-terminal GST tag in E. coliDepositorInsertUV excision repair protein RAD23 homolog A (RAD23A Human)
UseTagsGSTExpressionBacterialMutationCoding sequence has been optimised for expression…Promotertac promoterAvailable sinceNov. 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCA528 SPASTIN , residues 108-200
Plasmid#180628PurposeBacterial expression for SPASTIN MIT, residues 108-200. Has N-term HIS-SUMO tag. Used for FP experiments. Internal ID: WISP21-10.DepositorInsertSPASTIN
UseTagsHIS-SUMOExpressionBacterialMutationResidues 108-200PromoterT7Available sinceJuly 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGEX2T VPS4B, residues 1-86
Plasmid#180639PurposeBacterial expression for VPS4B MIT domain, residues 1-86. Has N-terminal GST tag. Internal ID: WISP04-155.DepositorInsertVPS4B (VPS4B Human)
UseTagsGSTExpressionBacterialMutationResidues 1-86PromoterAvailable sinceJuly 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pFP.R272
Plasmid#138221PurposeExpresses the split mCherry reporter interrupted by the SspGyrB artificially mini-intein (in cis)DepositorInsertsUseSynthetic BiologyTags6xHisExpressionBacterialMutationPromoterP(araBAD) and iPCAvailable sinceMarch 25, 2020AvailabilityAcademic Institutions and Nonprofits only