30,263 results

1. pBL090J

   Plasmid

   #195045

   Purpose

   cCadC-CCB3.5 under the control of PBAD

   Depositor

   Tina Wang

   Article

   Lee et al ACS Synth Biol. 2022 Oct 21;11(10):3198-

   Insert

   cCadC-CCB3.5
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Feb. 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pUC-GFP-AT

   Plasmid

   #133306

   Purpose

   "Burdensome" GFP expressing plasmid carrying axe/txe toxin-antitoxin for plasmid stabilisation

   Depositor

   Chris Barnes

   Article

   Fedorec et al iScience. 2019 Apr 26;14:323-334. doi: 1

   Insert

   axe/txe toxin-antitoxin cassette
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Nov. 25, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pNCST-AvicFP1

   Plasmid

   #129504

   Purpose

   Expresses AvicFP1 constitutively in E. coli (most strains)

   Depositor

   Nathan Shaner

   Article

   Lambert et al PLoS Biol. 2020 Nov 2;18(11):e3000936. d

   Insert

   AvicFP1
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   synthetic constitutive (stationary phase) promote…

   Available since

   Feb. 12, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pNCST-AvicFP4

   Plasmid

   #129507

   Purpose

   Expresses AvicFP4 constitutively in E. coli (most strains)

   Depositor

   Nathan Shaner

   Article

   Lambert et al PLoS Biol. 2020 Nov 2;18(11):e3000936. d

   Insert

   AvicFP4
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   One of two variants of AvicFP4 tested with essent…

   Promoter

   synthetic constitutive (stationary phase) promote…

   Available since

   Feb. 19, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pGEX-DAXX-ΔD/E-His

   Plasmid

   #179618

   Purpose

   Express GST-DAXX-ΔDE-His in bacteria

   Depositor

   Xiaolu Yang

   Article

   Huang et al Nature. 2021 Aug 18. pii: 10.1038/s41586

   Insert

   death domain associated protein (DAXX Human)
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   deleted amino acids 424-495

   Promoter

   Available since

   Feb. 18, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pNCST-AvicFP2

   Plasmid

   #129505

   Purpose

   Expresses AvicFP2 constitutively in E. coli (most strains)

   Depositor

   Nathan Shaner

   Article

   Lambert et al PLoS Biol. 2020 Nov 2;18(11):e3000936. d

   Insert

   AvicFP2
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   synthetic constitutive (stationary phase) promote…

   Available since

   Feb. 19, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pABE8e-protein

   Plasmid

   #161788

   Purpose

   Rhamnose-inducible expression of 8xHIS-ABE8e in E. coli

   Depositor

   David Liu

   Article

   Huang et al Nat Protoc. 2021 Feb;16(2):1089-1128. do

   Insert

   ABE8e base editor
   

   Use

   Tags

   8xHIS and BPNLS

   Expression

   Bacterial

   Mutation

   Promoter

   pRhaBAD

   Available since

   June 22, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pJUMP49-2A(sfGFP)

   Plasmid

   #126964

   Purpose

   Level 2 vector. OriV 9 (pBBR322/ROP; medium copy number); sfGFP cloning reporter.

   Depositor

   Chris French

   Article

   Valenzuela-Ortega et al Synth Biol (Oxf). 2021 Feb 2;6(1):ysab00

   Type

   Empty backbone

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   None

   Promoter

   Available since

   July 31, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pJUMP29-1A(sfGFP)

   Plasmid

   #126960

   Purpose

   Level 1 vector. OriV 9 (pBBR322/ROP; medium copy number); sfGFP cloning reporter.

   Depositor

   Chris French

   Article

   Valenzuela-Ortega et al Synth Biol (Oxf). 2021 Feb 2;6(1):ysab00

   Type

   Empty backbone

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   None

   Promoter

   Available since

   June 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pCPP6534

    Plasmid

    #128735

    Purpose

    Gateway destination pUC18R6KT-Tn7T-PavrPto-GW-Cya-Km vector for integration into DC3000D36E genome glmS-linked attTn7 site any effector gene, expressed from avrPto promoter and with C-terminal HA tag

    Depositor

    Alan Collmer

    Article

    Wei et al Cell Rep. 2018 May 8;23(6):1630-1638. do

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pEasyG2_hph

    Plasmid

    #184915

    Purpose

    Template to generate via PCR two gRNAs for expression in S. cerevisiae. The PCR product from pEasyG2_hph recombines in vivo with a PCR product from pEasyG2_mic.

    Depositor

    Jeferson Gross

    Article

    Jacobus et al ACS Synth Biol. 2022 Nov 18;11(11):3886-

    Insert

    gRNA scaffold
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pEasyG2_mic

    Plasmid

    #184916

    Purpose

    Template to generate via PCR two gRNAs for expression in S. cerevisiae. The PCR product from pEasyG2_mic recombines in vivo with a PCR product from pEasyG2_zeo/nat/hph.

    Depositor

    Jeferson Gross

    Article

    Jacobus et al ACS Synth Biol. 2022 Nov 18;11(11):3886-

    Insert

    gRNA scaffold
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pJUMP49-2E(sfGFP)

    Plasmid

    #126995

    Purpose

    Level 2 vector. OriV 9 (pBBR322/ROP; medium copy number); sfGFP cloning reporter.

    Depositor

    Chris French

    Article

    Valenzuela-Ortega et al Synth Biol (Oxf). 2021 Feb 2;6(1):ysab00

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    None

    Promoter

    Available since

    June 28, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. RpHLuorin2

    Plasmid

    #180227

    Purpose

    for bacterial expression of recombinant ratiometric pHluorin2

    Depositor

    Geert van den Bogaart

    Article

    Linders et al ACS Chem Biol. 2022 Jan 21;17(1):240-251

    Insert

    Ratiometric pHLuorin2
    

    Use

    Tags

    His-tag followed by thrombin cleavage site

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    March 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pGEX-DAXX-D/E-His

    Plasmid

    #179619

    Purpose

    Express DAXX D/E region in bacteria

    Depositor

    Xiaolu Yang

    Article

    Huang et al Nature. 2021 Aug 18. pii: 10.1038/s41586

    Insert

    death domain associated protein (DAXX Human)
    

    Use

    Tags

    GST, His, and TEV

    Expression

    Bacterial

    Mutation

    amino acids 414-505 of DAXX

    Promoter

    Available since

    Feb. 18, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pJUMP29-1D(sfGFP)

    Plasmid

    #126963

    Purpose

    Level 1 vector. OriV 9 (pBBR322/ROP; medium copy number); sfGFP cloning reporter.

    Depositor

    Chris French

    Article

    Valenzuela-Ortega et al Synth Biol (Oxf). 2021 Feb 2;6(1):ysab00

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    None

    Promoter

    Available since

    June 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pJUMP29-1C(sfGFP)

    Plasmid

    #126962

    Purpose

    Level 1 vector. OriV 9 (pBBR322/ROP; medium copy number); sfGFP cloning reporter.

    Depositor

    Chris French

    Article

    Valenzuela-Ortega et al Synth Biol (Oxf). 2021 Feb 2;6(1):ysab00

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    None

    Promoter

    Available since

    June 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pJUMP29-1B(sfGFP)

    Plasmid

    #126961

    Purpose

    Level 1 vector. OriV 9 (pBBR322/ROP; medium copy number); sfGFP cloning reporter.

    Depositor

    Chris French

    Article

    Valenzuela-Ortega et al Synth Biol (Oxf). 2021 Feb 2;6(1):ysab00

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    None

    Promoter

    Available since

    June 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pET His6 Thioredoxin TEV LIC cloning vector (2T-T)

    Plasmid

    #29712

    Depositor

    Scott Gradia

    Type

    Empty backbone

    Use

    Tags

    His6-Thioredoxin-TEV

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    June 9, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pJUMP29-1E(sfGFP)

    Plasmid

    #126982

    Purpose

    Level 1 vector. OriV 9 (pBBR322/ROP; medium copy number); sfGFP cloning reporter.

    Depositor

    Chris French

    Article

    Valenzuela-Ortega et al Synth Biol (Oxf). 2021 Feb 2;6(1):ysab00

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    None

    Promoter

    Available since

    June 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge