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Plasmid#159469PurposeEncodes for EF1p_mFAP2b with an N-terminal 6xHis tag, thrombin epitope, and TEV epitope. Expression is controlled by the T7 promoter with E. coli codon optimization.DepositorInsertEF1p_mFAP2b
UseTags6xHis-thrombin-TEVExpressionBacterialMutationPromoterAvailable sinceDec. 22, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBT29-splitD
Plasmid#122599Purposeclone M13 phage by SapI Golden GateDepositorInsertM13 bacteriophage genes
UseTagsExpressionBacterialMutationPromoterAvailable sinceJuly 29, 2019AvailabilityAcademic Institutions and Nonprofits only -
RanBP2-RB3-4-pET23a
Plasmid#85769Purposebacterial expression of RanBP2 SUMO E3 ligase fragment (aa 2304-3062) with C-terminal His tagDepositorInsertRanBP2 fragment (RANBP2 Human)
UseTagsHisExpressionBacterialMutationfragment (amino acids 2304-3062)PromoterT7Available sinceJan. 4, 2017AvailabilityAcademic Institutions and Nonprofits only -
pSL0426 (CRISPR with spc3)
Plasmid#130642PurposeExpresses CRISPR RNA from a T7 promoter for VchCAST system. The CRISPR array encodes a guide RNA with spacer 3 from the native CRISPR.DepositorInsertCRISPR(spacer-3)
UseCRISPR; TransposonTagsExpressionBacterialMutationPromoterT7Available sinceAug. 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pQTEV-YWHAE
Plasmid#31562DepositorInserttyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, epsilon (YWHAE Human)
UseTagsHis and TEVExpressionBacterialMutationPromoterAvailable sinceMarch 22, 2013AvailabilityAcademic Institutions and Nonprofits only -
ASB9A-c009
Plasmid#53670Purposebacterial expression plasmidDepositorInsertASB9A
UseTagsHis6-TEVExpressionBacterialMutationBacterial expression for structure determination;…PromoterAvailable sinceJune 3, 2014AvailabilityAcademic Institutions and Nonprofits only -
pGEX4T1-GST-eIF4G1-His6
Plasmid#37231DepositorInsertTIF4631 (TIF4631 Budding Yeast)
UseTagsGST and His6ExpressionBacterialMutationPromotertacAvailable sinceSept. 4, 2012AvailabilityAcademic Institutions and Nonprofits only -
pHis-zfCSF3b
Plasmid#65613Purposeexpress soluble His-tagged zebrafish CSF3b/Gcsfb in E. coliDepositorInsertCsf3b (csf3b Zebrafish)
UseTags(His)6ExpressionBacterialMutationPromoterT7Available sinceAug. 24, 2015AvailabilityAcademic Institutions and Nonprofits only -
pBAD/HisB-PhoCl-MBP
Plasmid#87689PurposeExpress PhoCl-MBP in E.coliDepositorInsertPhoCl fused to Maltose-Binding Protein
UseTagsExpressionBacterialMutationPromoterAvailable sinceApril 26, 2017AvailabilityAcademic Institutions and Nonprofits only -
pES133
Plasmid#127552PurposeVariant of pKD13. Lambda Red recombineering PCR template plasmid carrying an F5-tetA-F5 cassette orthogonal to FRT-KanR-FRT in pKD13DepositorInsertF5-flanked tetracycline resistance cassette
UseTagsExpressionBacterialMutationMultiple SNPs in tetA (originally from pBR322) to…PromoterAvailable sinceJuly 30, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCAG-Neo-PuroDTK
Plasmid#84036PurposePiggyBac cassette contains Neomycin, puromycin and TK genesDepositorTypeEmpty backboneUseTagsExpressionBacterial and MammalianMutationPromoterCAG promoterAvailable sinceApril 14, 2017AvailabilityAcademic Institutions and Nonprofits only -
pET28a-MinC
Plasmid#133628PurposeExpression vector for the Escherichia coli MinC with an N-terminal fusion of a His-tag for purification.DepositorInsertHis-MinC (minC E. coli)
UseTagsHisExpressionBacterialMutationPromoterAvailable sinceFeb. 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
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pSW003
Plasmid#111249PurposeBroad host-range bacterial vectorDepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceJuly 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
pJB37
Plasmid#202602PurposeArabinose inducible expression vector expressing retron Rcat-Sen2DepositorInsertRcaT-Sen2 toxin
UseTagsExpressionBacterialMutationPromoterAvailable sinceJuly 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLMB509
Plasmid#40084DepositorInsertsgfpmut 3.1
tauR
tau promoter
UseTagsExpressionBacterialMutationPromoterAvailable sinceNov. 8, 2012AvailabilityAcademic Institutions and Nonprofits only -
pGEX Fer-SH2
Plasmid#46425DepositorInsertFer (FER Human)
UseTagsGST and PreScissionExpressionBacterialMutationcontains amino acids 450-558PromoterTacAvailable sinceJuly 10, 2013AvailabilityAcademic Institutions and Nonprofits only -
pMyCA-mCherry
Plasmid#84272PurposemCherry fluorescence protein expression using M. smegmatisDepositorInsertmCherry
UseTagsHis6ExpressionBacterialMutationPromoterAcetamidaseAvailable sinceSept. 29, 2017AvailabilityAcademic Institutions and Nonprofits only -
pFPV25
Plasmid#20667DepositorTypeEmpty backboneUseTagsGFP mut3ExpressionBacterialMutationPromoterAvailable sinceMarch 5, 2009AvailabilityAcademic Institutions and Nonprofits only -
pHybr_r2a>mG2a-srt-his
Plasmid#124803PurposeHDR template to knock-in mouse IgG2a Fc domain in rat IgG2a heavy chain locus. Use in combination with PX458-gR2A_ISO to convert rat IgG2a hybridoma to mouse IgG2a producing cell lines.DepositorInsertMurine IgG2a
UseCRISPR; Hdr templateTagsHistag (HHHHHH) and Sortag (LPETGG)ExpressionBacterial and MammalianMutationPromoterAvailable sinceSept. 9, 2019AvailabilityAcademic Institutions and Nonprofits only