Showing: 6921 - 6940 of 27115 results
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Plasmid#72538PurposeProduces Acetobacter aceti 1023 formylglycinamidine ribonucleotide synthetase large subunit with N-terminal His6-tag (H6AaPurL)DepositorInsertformylglycinamidine ribonucleotide synthetase, large subunit
UseTagsHis6ExpressionBacterialMutationPromoterT7AvailabilityAcademic Institutions and Nonprofits only -
pJK624
Plasmid#72539PurposeProduces Acetobacter aceti 1023 OrfY; formylglycinamidine ribonucleotide synthetase glutaminase subunit; large subunit with C-terminal His6 tag (AaOrfY, AaPurQ, AaPurLH6)DepositorInsertshypothetical protein in purSQL operon
formylglycinamidine ribonucleotide synthetase, glutaminase subunit
formylglycinamidine ribonucleotide synthetase, large subunit
UseTagsHis6ExpressionBacterialMutationsynthetic genePromoterT7 and n/aAvailabilityAcademic Institutions and Nonprofits only -
pJK623
Plasmid#72540PurposeProduces Acetobacter aceti 1023 purSyQL associated hypothetical protein; formylglycinamidine ribonucleotide synthetase glutaminase subunit; large subunit (AaOrfY, AaPurQ, AaPurL)DepositorInsertshypothetical protein in purSQL operon
formylglycinamidine ribonucleotide synthetase, glutaminase subunit
formylglycinamidine ribonucleotide synthetase, large subunit
UseTagsExpressionBacterialMutationsynthetic genePromoterT7 and n/aAvailabilityAcademic Institutions and Nonprofits only -
pJK625
Plasmid#72541PurposeProduces Acetobacter aceti 1023 formylglycinamidine ribonucleotide synthetase small subunit with N-terminal His6 tag (H6AaPurS); glutaminase subunit (AaPurQ); large subunit (AaPurL)DepositorInsertshypothetical protein in purSQL operon
formylglycinamidine ribonucleotide synthetase, glutaminase subunit
formylglycinamidine ribonucleotide synthetase, large subunit
UseTagsHis6ExpressionBacterialMutationsynthetic genePromoterT7 and n/aAvailabilityAcademic Institutions and Nonprofits only -
pTRC-APEX2
Plasmid#72558PurposeExpression of cytosolic APEX2 in bacteriaDepositorInsertAPEX2
UseTagsHis6ExpressionBacterialMutationA134P relative to APEXPromoterAvailabilityAcademic Institutions and Nonprofits only -
pEN_TT 3XFLAG-wtGATA6-3XAU1
Plasmid#72612PurposeGateway entry vector for an inducible N-terminally 3XFLAG-tagged and C-terminally AU1-tagged human wtGATA6DepositorInsertGATA binding protein 6 (GATA6 Human)
UseEntry vector for gateway cloningTags3XAU1 and 3XFLAGExpressionBacterialMutationPromoterTRE tight promoterAvailabilityAcademic Institutions and Nonprofits only -
pEN_TT 3XFLAG-S37A-GATA6-3XAU1
Plasmid#72613PurposeGateway entry vector for an inducible N-terminally 3XFLAG-tagged and C-terminally AU1-tagged S37A mutant human GATA6DepositorInsertGATA binding protein 6 (GATA6 Human)
UseEntry vector for gateway cloningTags3XAU1 and 3XFLAGExpressionBacterialMutationChanged serine37 to alaninePromoterTRE tight promoterAvailabilityAcademic Institutions and Nonprofits only -
pJB051 (mEos2-ZapA)
Plasmid#72650PurposeInducible expression of mEos2-ZapA in bacteriaDepositorInsertmEos2-ZapA
UseTagsmEos2ExpressionBacterialMutationPromoterT5-lacAvailabilityAcademic Institutions and Nonprofits only -
pJB045 (ZapB-mEos2)
Plasmid#72651PurposeInducible expression of ZapB-mEos2 in bacteriaDepositorInsertZapB-mEos2
UseTagsmEos2ExpressionBacterialMutationPromoterT5-lacAvailabilityAcademic Institutions and Nonprofits only -
pXY029 (mEos2-MTSBs)
Plasmid#72652PurposeInducible expression of mEos2-MTSBs in bacteriaDepositorInsertmEos2-MTSBs
UseTagsmEos2ExpressionBacterialMutationPromoterT5-lacAvailabilityAcademic Institutions and Nonprofits only -
pJB128 (MatP-mEos2)
Plasmid#72653PurposeInducible expression of MatP-mEos2 in bacteriaDepositorInsertMatP-mEos2
UseTagsmEos2ExpressionBacterialMutationPromoterT5-lacAvailabilityAcademic Institutions and Nonprofits only -
pLicC-MBP-APETx2
Plasmid#72668PurposePeriplasmic expression of APETx2 in E.coliDepositorInsertAPETx2
UseTagsHis tag, MBP, MalE signal sequence for periplasmi…ExpressionBacterialMutationPromoterT7AvailabilityAcademic Institutions and Nonprofits only -
pKPY115
Plasmid#72669PurposeEncodes Thr251Gly-EcPheRS Under Arabinose-Inducible (PBAD) ControlDepositorInsertThr251Gly-EcPheRS
UseTagsExpressionBacterialMutationThr251Gly in Alpha SubunitPromoterPBADAvailabilityAcademic Institutions and Nonprofits only -
pCas1+2
Plasmid#72676PurposeExpress Cas1 and Cas2 for the CRISPR adaptation process in Escherichia coli. Has streptomycin resistance.DepositorInsertCas1, Cas2
UseTagsExpressionBacterialMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pORTMAGE-2
Plasmid#72677PurposeExpresses Lambda Red recombinases and a dominant negative MutL allele all controlled by temperature sensitve cI857 repressor for high precision and efficiency MAGE experiments. Ap resistance marker.DepositorInsertmutL E32K
UseTagsnoneExpressionBacterialMutationE32K mutation conferring dominant mutator phenoty…PromoterpL promoterAvailabilityAcademic Institutions and Nonprofits only -
pORTMAGE-3
Plasmid#72678PurposeExpresses Lambda Red recombinases and a dominant negative MutL allele all controlled by temperature sensitve cI857 repressor for high precision and efficiency MAGE experiments. Kan resistance marker.DepositorInsertmutL E32K
UseTagsnoneExpressionBacterialMutationE32K mutation conferring dominant mutator phenoty…PromoterAvailabilityAcademic Institutions and Nonprofits only -
pORTMAGE-4
Plasmid#72679PurposeExpresses Lambda Red recombinases and a dominant negative MutL allele all controlled by temperature sensitve cI857 repressor for high precision and efficiency MAGE experiments. Cam resistance marker.DepositorInsertmutL E32K
UseTagsnoneExpressionBacterialMutationE32K mutation conferring dominant mutator phenoty…PromoterAvailabilityAcademic Institutions and Nonprofits only -
pORTMAGE-1
Plasmid#72680PurposeSame as pORTMAGE-2, but with a non-optimized RBS before mutant MutL, thus lower level of expression of the enzyme.DepositorInsertmutL E32K
UseTagsnoneExpressionBacterialMutationlower strength RBS before mutL E32K allele than i…PromoterAvailabilityAcademic Institutions and Nonprofits only -
PatoG-OG241
Plasmid#72839PurposeInduced by acetoacetate to express GFP using atoSC two-component system in E. coli. Contains the native atoDAEB promoter (Pato) from E. coli DH5A. OG241 backbone.DepositorInsertPatoG
UseTagsExpressionBacterialMutationPromoterPatoGAvailabilityAcademic Institutions and Nonprofits only -
PatoK-OG241
Plasmid#72840PurposeNOT induced by acetoacetate to express GFP. Contains the native atoDAEB promoter (Pato) from E. coli DH5A, but out-of-frame with GFP gene. OG241 backbone.DepositorInsertPatoK
UseTagsExpressionBacterialMutationPromoterPatoKAvailabilityAcademic Institutions and Nonprofits only
Showing: 6921 - 6940 of 27115 results