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Plasmid#159403PurposeInducible superfolder GFP (sfGFP) expression, controlled under T7 bacteriophage promoterDepositorInsertpr.T5-LacO-6xHis-sfGFP
UseTags6xHisExpressionBacterialMutationPromoterT5 promoterAvailable sinceMarch 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
SLK_HUMAN_D0
Plasmid#79733PurposeThis plasmid encodes the kinase domain of SLK. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.DepositorInsertSLK (SLK Human)
UseTagsHis10-TEVExpressionBacterialMutationPromoterT7Available sinceNov. 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
SOCS2A-c016
Plasmid#53689Purposebacterial expression plasmidDepositorInsertSOCS2A
UseTagsHis6-TEVExpressionBacterialMutationBacterial expression for structure determination;…PromoterAvailable sinceJune 3, 2014AvailabilityAcademic Institutions and Nonprofits only -
pOT2
Plasmid#14460DepositorInsertGfp-UV
UseTagsExpressionBacterialMutationPromoterAvailable sinceApril 23, 2007AvailabilityAcademic Institutions and Nonprofits only -
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pUC19-T7-Top-T
Plasmid#87309PurposeExpresses the "Top" transcript of the Split-Broccoli system under the control of the T7 promoter.DepositorInsertSplit-Broccoli system "Top" transcript
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterT7Available sinceMay 23, 2017AvailabilityAcademic Institutions and Nonprofits only -
pETM6-FAS
Plasmid#49810PurposeEncodes CnfatB2, fabA, fabH, fabG, fabI in pseudo-operon configurationDepositorInsertsfatty acyl-ACP thioesterase
hydroxydecanoyl-ACP dehydratase
b-ketoacyl-ACP synthase III
3-ketoacyl-ACP reductase
enoyl-(acyl carrier protein) reductase
UseTagsExpressionBacterialMutationCodon-optmized. See supplemental information of …PromoterT7Available sinceFeb. 14, 2014AvailabilityAcademic Institutions and Nonprofits only -
LbCas12a-gRNA_BbsI_CAM
Plasmid#183222PurposeLbCas12a gRNA containing BbsI restriction recognition sites in spacer sequence for Golden Gate AssemblyDepositorInsertLbCas12a guide RNA containing BbsI sites in spacer sequence
UseCRISPRTagsExpressionBacterialMutationPromoterAvailable sinceJune 2, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET28a(TIGIT)
Plasmid#177902PurposeBacterial expression of his10-tagged human TIGIT Intracellular DomainDepositorInsertTIGIT (aa 163-244 only) (TIGIT Human)
UseTags10xHis-Thrombin cut site-T7 tagExpressionBacterialMutationPromoterT7Available sinceOct. 18, 2023AvailabilityAcademic Institutions and Nonprofits only -
pFP.R269
Plasmid#138181PurposeExpresses the split mCherry reporter interrupted by the NrdJ-1 intein (in cis)DepositorInsertsUseSynthetic BiologyTags6xHisExpressionBacterialMutationPromoterP(araBAD) and iPCAvailable sinceMay 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
pUCBB-pT7-eGFP
Plasmid#32552DepositorInserteGFP (eGFP )
UseSynthetic BiologyTagsHisExpressionBacterialMutationPromoterpT7Available sinceOct. 18, 2011AvailabilityAcademic Institutions and Nonprofits only -
pcNB2
Plasmid#89242PurposeResurfaced Cell-penetrating 5F7 nanobodyDepositorInsertpcNB2 (5F7)
UseTagsExpressionBacterialMutationPromoterT7Available sinceOct. 30, 2017AvailabilityAcademic Institutions and Nonprofits only -
pGMCtKq28-TSC10M1-sspB
Plasmid#49526PurposeKanamycin resistant; contains qtag28, expresses sspB under a promoter containing PtetO, and constutively expresses single-chain TetR10 (tsc10); integrates into the mycobacteriophage att-Tweety siteDepositorInsertTSC10M1-sspB
UseTagsExpressionBacterialMutationPromoterAvailable sinceJan. 17, 2014AvailabilityAcademic Institutions and Nonprofits only -
pET-S9
Plasmid#132948PurposeExpresses N-terminal 6xHis-tagged S9 ribosomal protein in E.coliDepositorInsert30S ribosomal subunit protein S9 (rpsI E.coli)
UseTagsN-terminal 6xHis-tagExpressionBacterialMutationE52CPromoterT7Available sinceMay 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTE103
Plasmid#59398PurposeMethyloBrick expression vector, fumC promoter; TcRDepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionBacterialMutationPromoterPfumCAvailable sinceDec. 30, 2014AvailabilityAcademic Institutions and Nonprofits only -
pGEX-4T1-USP11
Plasmid#78849PurposeTo purify GST-tagged recombinant USP11 proteinDepositorInsertUSP11 (USP11 Human)
UseTagsGSTExpressionBacterialMutationPromotertacAvailable sinceJune 15, 2016AvailabilityAcademic Institutions and Nonprofits only -
pBAD/HisB-PhoCl
Plasmid#87688PurposeExpress PhoCl in E. coliDepositorInsertPhoCl
UseTags6xHisExpressionBacterialMutationPromoterAvailable sinceApril 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
pNBU2_erm_P1T_DP-GH023 - NanoLuc
Plasmid#117727PurposeIntegration vector at an attB site in Bacteroides; contains the P1T_DP aTC-inducible promoter with the RBS GH023 upstream of the NanoLuc reporterDepositorInsertNanoLuc
UseLuciferaseTagsExpressionBacterialMutationPromoterAvailable sinceDec. 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET24c(+)-EFTu
Plasmid#31172DepositorInsertE. coli elongation factor Tu (tufB E. coli)
UseTagsHisExpressionBacterialMutationPromoterAvailable sinceSept. 30, 2011AvailabilityAcademic Institutions and Nonprofits only -
pMAL294
Plasmid#167834PurposeEncodes OptoLAC1 circuitDepositorInsertlacI
UseSynthetic BiologyTagsssrA (LAA)ExpressionBacterialMutationPromoterAvailable sinceAug. 18, 2021AvailabilityAcademic Institutions and Nonprofits only