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Addgene
Showing: 6881 - 6900 of 27115 results

  1. pJK626

    Plasmid
    #72461
    Purpose
    Produces Acetobacter aceti 1023 N5-carboxyaminoimidazole ribonucleotide mutase, Ser57>Cys/Tyr154>Phe mutant (AaPurE1-S57C,Y154F)
    Depositor
    T. J. Kappock
    Insert
    N5-carboxyaminoimidazole ribonucleotide mutase
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Mutation changes serine-57 to cysteine, tyrosine-…
    Promoter
    T7
    Availability
    Academic Institutions and Nonprofits only
    72461-plasmid-map-sequence-id-133899

    Enlarge

  2. pJK627

    Plasmid
    #72462
    Purpose
    Produces Acetobacter aceti 1023 N5-carboxyaminoimidazole ribonucleotide mutase, Ser57>Asp/Tyr154>Phe mutant (AaPurE1-S57D,Y154F)
    Depositor
    T. J. Kappock
    Insert
    N5-carboxyaminoimidazole ribonucleotide mutase
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Mutation changes serine-57 to aspartate, tyrosine…
    Promoter
    T7
    Availability
    Academic Institutions and Nonprofits only
    72462-plasmid-map-sequence-id-133901

    Enlarge

  3. pJK628

    Plasmid
    #72463
    Purpose
    Produces Acetobacter aceti 1023 N5-carboxyaminoimidazole ribonucleotide mutase, Ser57>Ala/Tyr154>Phe mutant (AaPurE1-S57A,Y154F)
    Depositor
    T. J. Kappock
    Insert
    N5-carboxyaminoimidazole ribonucleotide mutase
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Mutation changes serine-57 to alanine, tyrosine-1…
    Promoter
    T7
    Availability
    Academic Institutions and Nonprofits only
    72463-plasmid-map-sequence-id-133902

    Enlarge

  4. pJK629

    Plasmid
    #72464
    Purpose
    Produces Acetobacter aceti 1023 N5-carboxyaminoimidazole ribonucleotide mutase, Ser57>Thr/Tyr154>Phe mutant (AaPurE1-S57T,Y154F)
    Depositor
    T. J. Kappock
    Insert
    N5-carboxyaminoimidazole ribonucleotide mutase
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Mutation changes serine-57 to threonine, tyrosine…
    Promoter
    T7
    Availability
    Academic Institutions and Nonprofits only
    72464-plasmid-map-sequence-id-133903

    Enlarge

  5. pJK632

    Plasmid
    #72465
    Purpose
    Produces Acetobacter aceti 1023 N5-carboxyaminoimidazole ribonucleotide mutase, Ser57>Val/Tyr154>Phe mutant (AaPurE1-S57V,Y154F)
    Depositor
    T. J. Kappock
    Insert
    N5-carboxyaminoimidazole ribonucleotide mutase
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Mutation changes serine-57 to valine, tyrosine-15…
    Promoter
    T7
    Availability
    Academic Institutions and Nonprofits only
    72465-plasmid-map-sequence-id-133904

    Enlarge

  6. pJK636

    Plasmid
    #72466
    Purpose
    Produces Treponema denticola ATCC 35405 PurE with a TEV protease-cleavable N-terminal His6 tag (H6tTdPurE2)
    Depositor
    T. J. Kappock
    Insert
    phosphoribosylaminoimidazole carboxylase
    Use
    Tags
    His6 and TEV cleavage site
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Availability
    Academic Institutions and Nonprofits only
    72466-plasmid-map-sequence-id-133767

    Enlarge

  7. pJK640

    Plasmid
    #72467
    Purpose
    Encodes Escherichia coli evgA
    Depositor
    T. J. Kappock
    Insert
    response regulator in two-component regulatory system with EvgS (evgA Escherichia coli str. K-12 substr. MG1655)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Availability
    Academic Institutions and Nonprofits only
    72467-plasmid-map-sequence-id-134013

    Enlarge

  8. pJK641

    Plasmid
    #72468
    Purpose
    Encodes Escherichia coli evgA (Plac noncoding)
    Depositor
    T. J. Kappock
    Insert
    response regulator in two-component regulatory system with EvgS (evgA Escherichia coli str. K-12 substr. MG1655)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    lac
    Availability
    Academic Institutions and Nonprofits only
    72468-plasmid-map-sequence-id-134015

    Enlarge

  9. pJK646

    Plasmid
    #72469
    Purpose
    Encodes Escherichia coli evgA (Plac coding)
    Depositor
    T. J. Kappock
    Insert
    response regulator in two-component regulatory system with EvgS (evgA Escherichia coli str. K-12 substr. MG1655)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    none
    Availability
    Academic Institutions and Nonprofits only
    72469-plasmid-map-sequence-id-134016

    Enlarge

  10. pJK652

    Plasmid
    #72470
    Purpose
    Produces Escherichia coli acetyl-CoA:oxalate CoA-transferase (yfdE) with N-terminal His6 tag, His233>Gly mutant (H6EcYfdE-H233G)
    Depositor
    T. J. Kappock
    Insert
    acetyl-CoA:oxalate CoA-transferase (yfdE Escherichia coli str. K-12 substr. MG1655)
    Use
    Tags
    His6
    Expression
    Bacterial
    Mutation
    Mutated histidine-233 to glycine
    Promoter
    T7
    Availability
    Academic Institutions and Nonprofits only
    72470-plasmid-map-sequence-id-134018

    Enlarge

  11. pJK217

    Plasmid
    #72471
    Purpose
    Produces Acetobacter aceti 1023 citrate synthase with N-terminal His6 tag (H6AaAarA)
    Depositor
    T. J. Kappock
    Insert
    citrate synthase
    Use
    Tags
    His6
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Availability
    Academic Institutions and Nonprofits only
    72471-plasmid-map-sequence-id-135051

    Enlarge

  12. pJK317

    Plasmid
    #72472
    Purpose
    puc118 modified to contain ribosome binding site
    Depositor
    T. J. Kappock
    Type
    Empty backbone
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    lac
    Availability
    Academic Institutions and Nonprofits only
    72472-plasmid-map-sequence-id-140449

    Enlarge

  13. pJK635

    Plasmid
    #72473
    Purpose
    pET28a modified to contain TEV protease fusion tag instead of T7 tag
    Depositor
    T. J. Kappock
    Type
    Empty backbone
    Use
    Tags
    His6 and TEV protease cleavage site
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Availability
    Academic Institutions and Nonprofits only
    72473-plasmid-map-sequence-id-132656

    Enlarge

  14. pJK383

    Plasmid
    #72474
    Purpose
    Produces Thermoplasma acidophilum citrate synthase with Asp317>Gly mutant (TpCS-D317G)
    Depositor
    T. J. Kappock
    Insert
    citrate synthase
    Use
    Tags
    Expression
    Bacterial
    Mutation
    changes tryptophan-17 to phenylalanine, tryptopha…
    Promoter
    recA
    Availability
    Academic Institutions and Nonprofits only
    72474-plasmid-map-sequence-id-140430

    Enlarge

  15. pJK436

    Plasmid
    #72477
    Purpose
    Produces Thermoplasma acidophilum citrate synthase
    Depositor
    T. J. Kappock
    Insert
    citrate synthase
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Availability
    Academic Institutions and Nonprofits only
    72477-plasmid-map-sequence-id-134619

    Enlarge

  16. pJK439

    Plasmid
    #72478
    Purpose
    Produces Thermoplasma acidophilum citrate synthase pseudo-dimer
    Depositor
    T. J. Kappock
    Insert
    citrate synthase (artificial dimer)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    inserted NSTCLVPRGSPV sequence between C- and N-t…
    Promoter
    T7
    Availability
    Academic Institutions and Nonprofits only
    72478-plasmid-map-sequence-id-134617

    Enlarge

  17. pJK522

    Plasmid
    #72510
    Purpose
    Encodes Acetobacter aceti 1023 formylglycinamidine ribonucleotide synthetase small subunit with N-terminal His6 tag (H6AaPurS); hypothetical protein with N-terminal His6 tag (H6AaOrfY), synthetic
    Depositor
    T. J. Kappock
    Inserts
    formylglycinamidine ribonucleotide synthetase, small subunit
    hypothetical protein in purSQL operon
    Use
    Tags
    His6
    Expression
    Bacterial
    Mutation
    synthetic gene
    Promoter
    T7 and n/a
    Availability
    Academic Institutions and Nonprofits only
    72510-plasmid-map-sequence-id-132535

    Enlarge

  18. pJK530

    Plasmid
    #72511
    Purpose
    Produces Acetobacter aceti 1023 hypothetical protein in purSyQL cluster with C-terminal His6 tag (AaOrfYH6)
    Depositor
    T. J. Kappock
    Insert
    hypothetical protein in purSQL operon
    Use
    Tags
    His6
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Availability
    Academic Institutions and Nonprofits only
    72511-plasmid-map-sequence-id-132537

    Enlarge

  19. pJK531

    Plasmid
    #72512
    Purpose
    Produces Acetobacter aceti 1023 hypothetical protein in purSyQL cluster (AaOrfY)
    Depositor
    T. J. Kappock
    Insert
    hypothetical protein in purSQL operon
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Availability
    Academic Institutions and Nonprofits only
    72512-plasmid-map-sequence-id-132538

    Enlarge

  20. pJK532

    Plasmid
    #72513
    Purpose
    Produces Acetobacter aceti 1023 formylglycinamidine ribonucleotide synthetase glutaminase subunit with N-terminal His6 tag (H6AaPurQ)
    Depositor
    T. J. Kappock
    Insert
    formylglycinamidine ribonucleotide synthetase, glutaminase subunit
    Use
    Tags
    His6
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Availability
    Academic Institutions and Nonprofits only
    72513-plasmid-map-sequence-id-132539

    Enlarge

Showing: 6881 - 6900 of 27115 results