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pGEX-E6AP Hect
Plasmid#165102Purposebacterial expression of a GST fusion of Human E6AP Hect domainDepositorInsertE6AP Hect (UBE3A Human)
UseTagsGST from plasmid.ExpressionBacterialMutationE6AP Hect domain Amino acids 495-738 (5'QLNP…PromoterAvailable sinceMarch 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
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pMJ915v2 + sfGFP
Plasmid#88918PurposeFor expression of modified SpyCas9 in e.coli.DepositorInsertsCas9
T7
UseCRISPRTagsExpressionBacterialMutationPromoterAvailable sinceMay 2, 2017AvailabilityAcademic Institutions and Nonprofits only -
p3.5VPIII.EGFP.IGR2.1
Plasmid#40865DepositorInsertMouse vasopressin gene (Avp Mouse)
UseTagsExpressionBacterialMutationPromoterAVPAvailable sinceNov. 2, 2012AvailabilityAcademic Institutions and Nonprofits only -
pShCAST(Cas12k-TniQ-TniQ)-sgRNA_entry (CJT12)
Plasmid#181788PurposeExpresses 3-component ShCAST containing a Cas12k-TniQ-TniQ fusion. New sgRNA spacer sequences can be added using Golden Gate assembly (via SapI sites).DepositorInsertShTnsB, ShTnsC, ShCas12k-ShTniQ-ShTniQ
UseTagsExpressionBacterialMutationnAniI = K227M, F80K, L232KPromoterLac and J23119Available sinceFeb. 18, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBbS7k-RFP
Plasmid#35321DepositorInsertRFP
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterT7Available sinceFeb. 17, 2012AvailabilityAcademic Institutions and Nonprofits only -
pGBdest-GST-PI3Kc1
Plasmid#187992PurposePlasmid for the protein expression of GST-3C-ATG14/Vps34/Vps15/BECN1 in a bacterial and insect expression system. Internal_Reference: SMC1326DepositorUseTagsGSTExpressionBacterial and InsectMutationPromoterAvailable sinceFeb. 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
HOXA4 (murine) HA-tag pET
Plasmid#8489DepositorInsertHOXA4 (Hoxa4 Mouse)
UseTagsHA and T7ExpressionBacterialMutationThr-2 changed to Ala-2 to form better ribosomal b…PromoterAvailable sinceMay 30, 2006AvailabilityAcademic Institutions and Nonprofits only -
pJM230 (pUC18T-miniTn7T-gm-rhaSR-PrhaBAD-stRBS-lacZ)
Plasmid#110560PurposeminiTn7 delivery plasmid with rhaSR-PrhaBAD inducible promoter and StRBS-lacZ insertDepositorInsertstRBS-lacZ
UseTagsExpressionBacterialMutationPromoterrhaSR-PrhaBADAvailable sinceMay 23, 2018AvailabilityAcademic Institutions and Nonprofits only -
Sender plasmid
Plasmid#127677PurposeEncodes the synthase LuxI for the quorum-sensing molecule C6-HSL.DepositorInsertLuxI
UseSynthetic BiologyTagsLVAExpressionBacterialMutationPromoterpT7Available sinceSept. 10, 2019AvailabilityAcademic Institutions and Nonprofits only -
"AND-gate" receiver plasmid
Plasmid#127676PurposeEncodes the transcriptional activator LuxR and GFP. LuxR has an pLlacO-1 promoter and the GFP has a pLux promoter.DepositorInsertLuxR, GFP
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterpLlacO-1 for LuxR, pLux for GFPAvailable sinceJuly 31, 2019AvailabilityAcademic Institutions and Nonprofits only -
Z-UBQLN2-7
Plasmid#166563PurposescFv of a human scaffold targeting Ubiquilin-2. Antigen coverage aa 1-624 of 624 aa, full-lengthDepositorInsertsingle chain-Fv, scFv
UseRecombinant antibody expressionTags3xFLAG, His6 and OmpA leader sequenceExpressionBacterialMutationPromoterLacZAvailable sinceMarch 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
pExp-RBD-CHis
Plasmid#195002PurposeProduction of SARS-CoV2 receptor binding domain in E. coli with C-terminal Avi-tagDepositorInsertRBD (S SARS-CoV-2)
UseTagsHis8ExpressionBacterialMutationPromoterT7lacAvailable sinceJan. 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET21c(+)-EFG1mt
Plasmid#31076DepositorInsertMature form human mitochondrial elongation factor G isoform 1 (GFM1 Human)
UseTagsHisExpressionBacterialMutationIncludes the coding sequence for the mature form.…PromoterAvailable sinceSept. 29, 2011AvailabilityAcademic Institutions and Nonprofits only -
pNB23
Plasmid#103058Purposemedium copy plasmid (pBR322 backbone) expressing the unstable GFP variant GFP-OVA under control of the hydrogen peroxide-inducible promoter katGpDepositorInsertGFP-OVA
UseTagsExpressionBacterialMutationPromoterhydrogen peroxide-inducible promoter katGpAvailable sinceNov. 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
HHM-iATPSnFR1.1
Plasmid#102547Purposeexpresses ATP sensor in bacteriaDepositorInsertiATPSnFR1.1
UseTagsExpressionBacterialMutationPromoterAvailable sinceJan. 10, 2019AvailabilityAcademic Institutions and Nonprofits only -
pHJL2
Plasmid#199377PurposeEncodes dual cistronic cytosolic GCaMP6f and mScarlet-I optimized for C. elegans expressionDepositorInsertGCaMP6f::SL2::mScarlet-I
UseTagsExpressionBacterialMutationno mutationsPromoterAvailable sinceApril 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET24a MDH_STRAW
Plasmid#204240PurposeBacterial expression of S. avermitilis malate dehydrogenase; Use in CURES (integration of research into undergraduate teaching labs).InsertMDH_STRAW (mdh Streptomyces avermitilis)
UseTags6X HisExpressionBacterialMutationPromoterLacAvailable sinceNov. 6, 2023AvailabilityAcademic Institutions and Nonprofits only
