-
-
pSR1037
Plasmid#208736PurposeCharacterization plasmid for PSarA1 (sfgfp expressed from PSarA1 on the S. aureus pC194 replicon)DepositorInsertPSarA1
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceDec. 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGEX6P1-A20 (OTU, aa 1-366)
Plasmid#61584PurposeExpresses human A20 (OTU domain) in E. coli.DepositorInsertA20 (TNFAIP3 Human)
UseTagsGST-3CExpressionBacterialMutationDeleted aa 367-790.PromotertacAvailable sinceMarch 12, 2015AvailabilityAcademic Institutions and Nonprofits only -
pHC01
Plasmid#106900PurposeRatiometric gas biosensor plasmid for 3-oxo-C12-HSL sensingDepositorInsertsLasR
Methyl Halide Transferase
Ethylene Forming Enzyme
Red Fluorescent Protein
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
Luciferase
Plasmid#213979PurposeExpresses Doxy Inducible LuciferaseDepositorInsertLuciferase
UseLentiviralTags6xHis affinity tagExpressionBacterial and MammalianMutationPromoterTet-responsive promoter PTight, consisting of sev…Available sinceApril 30, 2024AvailabilityAcademic Institutions and Nonprofits only -
ku70 insUP+pPGI+NAT+tNOS+ku70 insD (SBE217)
Plasmid#195049Purposeintegration cassette for ku70 deletion and expression of NAT gene under pPGI for characterizationDepositorInsertNAT
UseTagsExpressionBacterialMutationPromoterpPGIAvailable sinceNov. 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
pUS252L
Plasmid#191832PurposeExpresses fuBFP constitutively in E.coli cells. Designed to act as modular template for cutting out or amplifying fuBFP to place in other plasmid backbonesDepositorInsertfuBFP
UseTagsExpressionBacterialMutationPromoterAvailable sinceJan. 17, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits -
pCS-lpp0.2-K64A
Plasmid#201789Purposemutant of PadR; transcriptional regulator can respond to p-coumaric acidDepositorInsertPadR (padR Bacillus subtilis)
UseTagsExpressionBacterialMutationK64APromoterAvailable sinceJuly 12, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits -
pCS-lpp0.2-H38A
Plasmid#201788Purposemutant of PadR; transcriptional regulator can respond to p-coumaric acidDepositorInsertPadR (padR Bacillus subtilis)
UseTagsExpressionBacterialMutationH38APromoterAvailable sinceJuly 12, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits -
pZE-PpadC-yveF-yveG-egfp
Plasmid#201791Purposethe promoter can be recognized by PadRDepositorInsertPpadC-yveF-yveG
UseTagsExpressionBacterialMutationWTPromoterAvailable sinceJuly 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pZH514
Plasmid#102667PurposeConstitutive expression of GFPmut2; strong ribosome binding site; GFPmut2 ORF can be replaced with gene of interest by isothermal assemblyDepositorInsertGFPmut2
UseTagsExpressionBacterialMutationPromoterAvailable sinceDec. 11, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAJM.712
Plasmid#108513PurposePCymRC-YFP Reporter. Cuma sensor for use in Marionette strain.DepositorInsertPCymRC-YFP
UseTagsExpressionBacterialMutationPromoterAvailable sinceJune 30, 2018AvailabilityAcademic Institutions and Nonprofits only -
pRK1037
Plasmid#8833DepositorInsertTVMV protease, catalytic domain
UseTagsExpressionBacterialMutationPromoterAvailable sinceMay 30, 2006AvailabilityAcademic Institutions and Nonprofits only -
pYTK035
Plasmid#65142PurposeEncodes I-SceI (ORF) as a Type 3 part to be used in the Dueber YTK systemDepositorInsertI-SceI (ORF)
UseTagsExpressionBacterialMutationPromoterAvailable sinceJune 16, 2015AvailabilityAcademic Institutions and Nonprofits only -
pJK506
Plasmid#155382PurposedCas12a (D917A) + PA4-mVenus. (removed crRNAs)DepositorInsertsdCas12a (F. novicida)
PA4-mVenus
UseCRISPRTagsExpressionBacterialMutationD917A (nuclease-deactivating)PromoterAvailable sinceJan. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET21-pelB-LaG-41
Plasmid#172757PurposeBacterial expression of HIS-tagged anti-GFP nanobody LaG-41; HIS tag preceded by HRV 3C/PreScission cleavage site.DepositorInsertLaG-41
UseTags6xHIS and pelB leader for periplasmic secretionExpressionBacterialMutationPromoterT7Available sinceAug. 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
pFS_0271_pET30_pCat-tetR-Term-ptetA-sfGFP-rrnBterm_Fusicatenibacter_saccharivorans_ARRAY2_FaqI
Plasmid#116955PurposeExpresses FsRT-Cas1-Cas2 under pT7lac and sfGFP under ptetA promoter, encodes FsCRISPR array 2, compatible with SENECA acquisition readoutDepositorInsertFusicatenibacter saccharivorans RT-Cas1-Cas2
UseTagsExpressionBacterialMutationPromoterT7Available sinceNov. 2, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLOXp-GUS
Plasmid#154059PurposeLevel 0.5 Golden Gate vector, loxP-GUS-nosT-loxPDepositorInsertbeta-glucoronidase (E. coli)
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterN/AAvailable sinceSept. 29, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET-His6-Sumo-MBP-CfaN
Plasmid#126016PurposeBacterial expression of MBP-CfaN fusion protein (with N-terminal His5-Sumo)DepositorInsertHis5-Sumo-MBP-CfaN
UseTags5xHis-SUMOExpressionBacterialMutationPromoterAvailable sinceAug. 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCRISPR_eGFP_497
Plasmid#111824PurposeKnockout eGFPDepositorInsertgRNA targeting eGFP 477-497
UseCRISPRTagsmCherryExpressionBacterial and MammalianMutationPromoterU6Available sinceAug. 2, 2018AvailabilityAcademic Institutions and Nonprofits only
